Characterization of a Cold Atmospheric Pressure Helium Plasma Jet for Biomedical Experiments

Dieffenbach, Payson; Lauria, Michael; Shashurin, Alexey; Garner, Allen L.

doi:10.1109/plasma.2017.8496197

Cited by 1 publication

(1 citation statement)

References 4 publications

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“…Moreover, OH radicals can chemically react with the N terminus of the gap junction, destroying its structure [14]. On the other hand, CAP-generated ROS, such as H 2 O 2 and OH radicals, can also damage the phospholipid bilayer structure of cell membrane, resulting in lipid peroxidation [43,44], decrease in membrane potential [45], and fragmentation of the membrane structure [46]. In summary, CAP treatment can undoubtedly change intercellular junctions.…”

Section: Potential Mechanisms Of Cap Generated Rons Affecting Interce...mentioning

confidence: 99%

Uncertainty Quantification and Sensitivity Analysis for the Electrical Impedance Spectroscopy of Changes to Intercellular Junctions Induced by Cold Atmospheric Plasma

et al. 2022

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The influence of pertinent parameters of a Cole-Cole model in the impedimetric assessment of cell-monolayers was investigated with respect to the significance of their individual contribution. The analysis enables conclusions on characteristics, such as intercellular junctions. Especially cold atmospheric plasma (CAP) has been proven to influence intercellular junctions which may become a key factor in CAP-related biological effects. Therefore, the response of rat liver epithelial cells (WB-F344) and their malignant counterpart (WB-ras) was studied by electrical impedance spectroscopy (EIS). Cell monolayers before and after CAP treatment were analyzed. An uncertainty quantification (UQ) of Cole parameters revealed the frequency cut-off point between low and high frequency resistances. A sensitivity analysis (SA) showed that the Cole parameters, R0 and α were the most sensitive, while Rinf and τ were the least sensitive. The temporal development of major Cole parameters indicates that CAP induced reversible changes in intercellular junctions, but not significant changes in membrane permeability. Sustained changes of τ suggested that long-lived ROS, such as H2O2, might play an important role. The proposed analysis confirms that an inherent advantage of EIS is the real time observation for CAP-induced changes on intercellular junctions, with a label-free and in situ method manner.

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Section: Potential Mechanisms Of Cap Generated Rons Affecting Interce...mentioning

confidence: 99%

Uncertainty Quantification and Sensitivity Analysis for the Electrical Impedance Spectroscopy of Changes to Intercellular Junctions Induced by Cold Atmospheric Plasma

et al. 2022

View full text Add to dashboard Cite

show abstract

Characterization of a Cold Atmospheric Pressure Helium Plasma Jet for Biomedical Experiments

Cited by 1 publication

References 4 publications

Uncertainty Quantification and Sensitivity Analysis for the Electrical Impedance Spectroscopy of Changes to Intercellular Junctions Induced by Cold Atmospheric Plasma

Uncertainty Quantification and Sensitivity Analysis for the Electrical Impedance Spectroscopy of Changes to Intercellular Junctions Induced by Cold Atmospheric Plasma

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