Characterization of a chloroplast DNA sequence from Chlorella ellipsoidea that promotes autonomous replication in yeast

Yamada, Takashi; Shimaji, Miyuki; Fukuda, Yuji

doi:10.1007/bf00015230

Cited by 8 publications

(1 citation statement)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3, positions 2,543-2,766) as a probe showed the presence of some related sequences to the a-elements on several other parts of cpDNA and even on the nuclear DNA from Chlorella (Yamada and Shimaji, unpublished reuslt). Recently, we revealed the presence of such a repetitive sequence with SmaI sites (like a-element) in a Chlorella cpDNA fragment that shows ARS activity (26). Schneider et al reported in the flanking regions of the chloropolast rRNA unit of Chlamydomonas reinhardii numerous tandem and iverted repeats (6).…”

Section: Open Reading Framesmentioning

confidence: 93%

Peculiar feature of the organization of rRNA genes of theChlorellachloroplast DNA

Yamada

Shimaji

1986

Nucl Acids Res

Self Cite

View full text Add to dashboard Cite

The organization of a cloned rRNA gene cluster from Chlorella ellipsoidea chloroplast DNA (cpDNA) has been analyzed. Southern hybridization experiments with labelled chloroplast rRNAs as probes revealed an extraordinarily large size of the 16S-23S rRNA spacer region, ca. 4.8 kbp, almost twice as large as those of most higher plants. The nucleotide sequence determined on this region has shown that: (1) The tRNAIle gene locating in this region is similar to those of higher plant chloroplasts, blue-green algae and E. coli but does not contain any introns in contrast to higher plant chloroplasts.

show abstract

Section: Open Reading Framesmentioning

confidence: 93%

Peculiar feature of the organization of rRNA genes of theChlorellachloroplast DNA

Yamada

Shimaji

1986

Nucl Acids Res

Self Cite

View full text Add to dashboard Cite

show abstract

Electron microscopic localization of replication origins in Oenothera chloroplast DNA

Chiu

Sears

1992

Molec. Gen. Genet.

View full text Add to dashboard Cite

The origins of chloroplast DNA (cpDNA) replication were mapped in two plastome types of Oenothera in order to determine whether variation in the origin of cpDNA replication could account for the different transmission abilities associated with these plastomes. Two pairs of displacement loop (D-loop) initiation sites were observed on closed circular cpDNA molecules by electron microscopy. Each pair of D-loops was mapped to the inverted repeats of the Oenothera cpDNA by the analysis of restriction fragments. The starting points of the two adjacent D-loops are approximately 4 kb apart, bracketing the 16S rRNA gene. Although there are small DNA length variations near one of the D-loop initiation sites, no apparent differences in the number and the location of replication origins were observed between plastomes with the highest (type I) and lowest (type IV) transmission efficiencies.

show abstract

Repetitive sequence-mediated rearragements in Chlorella ellipsoidea chloroplast DNA: completion of nucleotide sequence of the large inverted repeat

Yamada

1991

Curr Genet

View full text Add to dashboard Cite

A 3454 base pair (bp) sequence of the large inverted repeat (IR) of chloroplast DNA (cpDNA) from the unicellular green alga Chlorella ellipsoidea has been determined. The sequence includes: (1) the boundaries between the IR and the large single copy (LSC) and the small single copy (SSC) regions, (2) the gene for psbA and (3) an approximately 1.0 kbp region between psbA and the rRNA genes which contains a variety of short dispersed repeats. The total size of the Chlorella IR was determined to be 15243 bp. The junction between the IR and the small single copy region is located close to the putative promoter of the rRNA operon (906 bp upstream of the -35 sequence on each IR). The junction between the IR and the large single copy region is also just upstream of the putative psbA promoter, 218 bp upstream from the ATG initiation codon. A few sets of unique sequences were found repeatedly around both junctions. Some of the sequences flanking the IR-LSC junction suggest a unidirectional and serial expansion of the IR within the genome. The psbA gene is located close to the LSC-side junction and codes for a protein of 352 amino acid residues. A highly conserved C-terminal Gly is absent Unlike the psbA of Chlamydomonas species, which contains 2-4 large introns, the gene of Chlorella has no introns.(ABSTRACT TRUNCATED AT 250 WORDS)

show abstract

Characterization of a chloroplast DNA sequence from Chlorella ellipsoidea that promotes autonomous replication in yeast

Cited by 8 publications

References 21 publications

Peculiar feature of the organization of rRNA genes of theChlorellachloroplast DNA

Peculiar feature of the organization of rRNA genes of theChlorellachloroplast DNA

Electron microscopic localization of replication origins in Oenothera chloroplast DNA

Repetitive sequence-mediated rearragements in Chlorella ellipsoidea chloroplast DNA: completion of nucleotide sequence of the large inverted repeat

Contact Info

Product

Resources

About