Characterization of a chitosan sample extracted from Brazilian shrimps and its application to obtain insoluble complexes with a commercial whey protein isolate

Studies on the interactions between bovine β-lactoglobulin and chitosan at the solid–liquid interface

“…Considering that the working conditions were chosen to disfavor the formation of weakly attached protein aggregates, the behavior observed in Fig. 5 is direct proof of the strong interactions between ␤-LG and chitosan, which agrees well with that seen in solution studies [21,22].…”

“…4 and 5, indicate that the adsorption of ␤-LG on chitosan occurs mostly in an irreversible way and that no phase transitions to reversibly adsorbed states seem to occur either after a critical time or coverage. This behavior is in agreement with results in solution [21,22] and illustrates the significant amounts of energy involved in ␤-LG-chitosan interacting forces. Table 3 Surface roughness (Rms) a and average height (Hav) a obtained from the 5 m × 5 m (Fig.…”

“…In our recent research we have performed calorimetric, spectroscopic, and rheological solution studies on the interactions between bovine WPI and chitosans (commercial or extracted from Brazilian schrimps) with different molar mass (M) and degree of deacetylation (DD) [21,22]. Those reports unveiled the important role of solution pH, DD, and WPI-chitosan molar ratio on the strength of the resulting interactions.…”

Calorimetric techniques applied to the thermodynamic study of interactions between proteins and polysaccharides

“…Similarly, the interactions between Chitosan and WPI by electrostatic bonds were dependent not only on the pH but also the ionic strength, and molar ratio (BASTOS et al, 2010).…”

Unique characteristics of self-assembly of bovine serum albumin and fucoidan, an anionic sulfated polysaccharide, under various aqueous environments

“…The latter phenomena give rise to the formation of complex coacervates, co-precipitates (interpolymeric complexes), gels, and soluble complexes (Turgeon, Beaulieu, Schmitt, & Sanchez, 2003). Until now, a large number of reviews have been published on the behaviors of solutions with protein/polysaccharide interactions (de Kruif, Weinbreck, & de Vries, 2004;Schmitt & Turgeon, 2011;Turgeon, Schmitt, & Sanchez, 2007;Veis, 2011) and there have been numerous original papers published on the formation of biopolymer complexes as a function of various parameters (Bastos et al, 2010;Bengoechea, Jones, Guerrero, & McClements, 2011;Elmer, Karaca, Low, & Nickerson, 2011;Fioramonti, Perez, Aríngoli, Rubiolo, & Santiago, 2014;Giancone, Torrieri, Masi, & Michon, 2009;Laneuville, Paquin, & Turgeon, 2000;Lee & Hong, 2009;Souza, Rojas, Melo, Gaspar, & Lins, 2013;Weinbreck, de Vries, Schrooyen, & de Kruif, 2003;Weinbreck, Nieuwenhuijse, Robijn, & de Kruif, 2003;Weinbreck, Nieuwenhuijse, Robijn, & de Kruif, 2004;Weinbreck, Tromp, & de Kruif, 2004). According to precedent studies, the driving force for these non-covalent interactions are the electrostatic interactions between oppositely charged biopolymers, which can be affected by both extrinsic factors (protein/polysaccharide ratio, pH, ionic strength, and temperature) and intrinsic factors (molecular weight, molecular structure, net charge and flexibility of chains).…”