Characterization of a chiral tripeptide stationary phase for the liquid chromatographic separation of chiral dipeptides

Howard, William A.; Hsu, Tau Being; Rogers, L. B.; Nelson, David A.

doi:10.1021/ac00280a010

Cited by 10 publications

(4 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has long been recognized that synthesis of CSPs by solid phase synthesis can be problematic. 5 Nevertheless, this approach allows rapid proof of principle. A CSP with further improved performance could probably be prepared by first synthesizing and purifying the chiral selector, then immobilizing it on silica.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Selection of an Optimized Adsorbent for Preparative Chromatographic Enantioseparation by Microscale Screening of a Second-Generation Chiral Stationary Phase Library

1999

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 99%

“…The resulting material was packed into a 4.6 × 250 mm HPLC column and evaluated chromatographically. It has long been recognized that synthesis of CSPs by solid phase synthesis can be problematic . Nevertheless, this approach allows rapid proof of principle.…”

Section: Resultsmentioning

confidence: 99%

Selection of an Optimized Adsorbent for Preparative Chromatographic Enantioseparation by Microscale Screening of a Second-Generation Chiral Stationary Phase Library

1999

View full text Add to dashboard Cite

“…Synthetic peptides have been used in both normal-and reversed-phase chromatography. For example,dipeptides have been separated in the RPC mode on an L-Val-L-Ala-L-Pro bonded phase with water or an aqueous buffer as the mobile phase [ 107,108]. The dipeptides elute in the order of increasing hydrophobicity of their hydrocarbon side-chains and the capacity factors increase with increasing pH.…”

Section: Chiral Separationsmentioning

confidence: 99%

Stationary phases for peptide analysis by high performance liquid chromatography: a review

Štulı́k¹,

Pacáková²,

Suchánková³

et al. 1997

Analytica Chimica Acta

View full text Add to dashboard Cite

A survey is given of modern stationary phases employed in high performance liquid chromatography (HPLC) analysis of peptides. The physico-chemical properties of peptides and their consequences for the selection and optimization of the separation system are briefly discussed, followed by a summary of the approaches to the selection and characterization of stationary phases. The properties and applicability of various stationary phases are then critically reviewed, including aspects such as size-exclusion, ion-exchange, reversed-phase, hydrophobic-interaction, affinity and chiral systems, as well as some specialized separation techniques. Emphasis is placed on the most recent literature. 0 1997 Elsevier Science B.V.

show abstract

“…Nevertheless, research on peptide and peptidomimetic chiral selectors has continued and significant progress has been made in recent years. Early work regarding peptide chiral selectors such as those from Grushka and Scott [7], Rogers et al [8,9], and Birkinshaw and Taylor [10] has been reviewed [11]. In this article, the use of combinatorial libraries to screen a large number of peptides and peptidomimetics for potential enantioselectivity, chiral diamide phases, and oligoproline-based chiral stationary phases are discussed.…”

Section: Introductionmentioning

confidence: 99%

Peptide and peptidomimetic chiral selectors in liquid chromatography

Li¹

2005

J of Separation Science

View full text Add to dashboard Cite

The application of peptide and peptidomimetic chiral selectors in LC is reviewed in this article. In particular, methods for finding these types of selectors using combinatorial library approaches are discussed, as well as recent advances in the use of peptides and peptidomimetics as general chiral selectors for LC. In terms of the library approaches, batch screening and reciprocal screening are discussed. As for general chiral selectors, one example involves the application of chiral diamide phases. Another example shows the versatility of oligoproline chiral stationary phases, which exhibit resolution for a number of racemic analytes, comparable to other well-established chiral stationary phases.

show abstract

Characterization of a chiral tripeptide stationary phase for the liquid chromatographic separation of chiral dipeptides

Cited by 10 publications

References 16 publications

Selection of an Optimized Adsorbent for Preparative Chromatographic Enantioseparation by Microscale Screening of a Second-Generation Chiral Stationary Phase Library

Selection of an Optimized Adsorbent for Preparative Chromatographic Enantioseparation by Microscale Screening of a Second-Generation Chiral Stationary Phase Library

Stationary phases for peptide analysis by high performance liquid chromatography: a review

Peptide and peptidomimetic chiral selectors in liquid chromatography

Contact Info

Product

Resources

About