Characterization of a Chiral-AGP capillary column coupled to a micro sample-enrichment system with UV and electrospray mass spectrometric detection

Hermansson, Jörgen; Hermansson, Inger; Nordin, Jan

doi:10.1016/0021-9673(93)80508-6

Cited by 14 publications

(15 citation statements)

References 21 publications

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“…Chiral separations can be achieved by the use of chiral stationary phases [265][266][267][268][269][270], a chiral selector that is adsorbed on the packing material [265,271,272], or by using chiral mobile phase additives [265,[273][274][275]. For instance, W~nnman et al [265] reported on the separation of chiral ethanol and propanol derivatives on a Pirkle phase.…”

Section: Chiral Separationsmentioning

confidence: 99%

Microcolumn liquid chromatography: instrumentation, detection and applications

Vissers¹,

Claessens

Cramers

1997

Journal of Chromatography A

208

101

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This review discusses many different aspects of microcolumn liquid chromatography (LC) and reflects the areas of microcolumn LC research interest over the past decades. A brief theoretical discussion on a number of major issues, such as column characterisation, chromatographic dilution effects and extracolumn band broadening in microcolumn LC is given. Recent progress in column technology and the demands and developments of instrumentation and accessories for microcolumn LC are also reviewed. Besides that, the developments in a large number of established and also more recent detection techniques for microcolumn LC are also discussed. The potential of hyphenation of microcolumn LC with other techniques, more particularly of multidimensional chromatography and microcolumn LC coupled to mass spectrometry is reviewed. Finally, the perspective of microcolumn LC separation methods is stressed by a number of relevant applications.

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Section: Chiral Separationsmentioning

confidence: 99%

Microcolumn liquid chromatography: instrumentation, detection and applications

Vissers¹,

Claessens

Cramers

1997

Journal of Chromatography A

208

101

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show abstract

“…Significant advances in the field of ionization techniques (especially atmospheric-pressure ionization methods) have resulted in a dramatic increase in the use of hyphenated LC-MS techniques in chemical and biomedical research, 13 and a number of reports have demonstrated the utility of LC-MS for the analysis of chiral pharmaceuticals. 14,15 Various chiral selectors have been applied in standardsize or miniaturized LC-MS for enantiomer separation; 15,16 these include proteins such as a-acid glycoprotein, [17][18][19] conalbumin, 20 human serum albumin, 21 and avidin. 22,23 As protein-based CSPs are typically operated in the reversed-phase mode (i.e., in aqueous buffers that may contain organic additives), transformation of chromatographic conditions from UV-to MS-detection can readily be achieved.…”

mentioning

confidence: 99%

Enantiomer separation of amino acids in immunoaffinity micro LC‐MS

et al. 2006

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Chiral immunoaffinity microbore columns were directly interfaced with MS detection, and the effect of column length and temperature on the enantiomer separation of a number of underivatized aromatic and aliphatic amino acids was investigated utilizing an antibody chiral stationary phase that had been prepared by immobilizing a monoclonal anti-D-amino acid antibody onto silica. The stronger affinity of the antibody towards aromatic and bulky amino acids allowed separation of such analytes in a 0.75 x 150 mm column, while an increase in column length enabled separation of more weakly bound compounds. The strength of interaction between chiral selector and analytes could be modulated conveniently by lowering the temperature. For the first time, simultaneous enantiomer separation of mixtures of amino acids was achieved on antibody-based chiral stationary phases using extracted ion chromatograms.

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“…A number of chiral LC/UV-Vis assays require inorganic buffers (e. g., phosphate buffer) and/or high aqueous mobile phases, which can potentially lead to significant ion-suppression during the LC-MS experiments. In order to address the former issue and overcome the biological matrix interference prior to MS ionization, elaborate multidimensional chromatography techniques and online desalting schemes have been proposed [121]. Sufficient online sample clean-up is particularly important when performing trace analysis; however, it may be timeconsuming and limits high-throughput analysis.…”

Section: Chiral Supramolecular-assisted Lc-msmentioning

confidence: 99%

“…The easiest chiral-LC methods to adapt to LC-MS formats are polar-organic mode separations [121], where the most useful additives are NH 4 TFA and NH 4 NO 3 . Lower detection limits have been found for ESI-MS using polar-organic phases because of the lower ionization efficiency of RP mode owing to the presence of water.…”

Section: Several Factors Should Be Considered When Developing An Immomentioning

confidence: 99%

Supramolecular systems‐based extraction‐separation techniques coupled to mass spectrometry

Merino

Rubio

Pérez‐Bendito³

2005

J of Separation Science

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The combination of supramolecular chemistry and MS has not only been fruitful in the field of gas-phase fundamental studies of host-guest complexes and supramolecular assemblies. Mass spectrometric analysis has also benefited from the ability of supramolecular systems to behave as pseudophases in which solutes partition from the bulk solvent phase. Supramolecular systems-based extraction and concentration schemes and separation techniques have been widely used in different fields of analytical chemistry and are ideally suited for coupling with MS. This review describes the present status of the application of supramolecular chemistry in mass spectrometric analysis and includes topics such as the use of coacervative liquid-liquid extraction and hemimicelle/admicelle-based SPE of organic compounds prior to chromatography and electrophoresis. It also discusses the recent advances in enantioselective analysis using CD in electrophoresis- and chromatography-MS. The potential and analytical challenges of these approaches in environmental and bioanalytical chemistry, where one can expect significant developments in the future, are outlined.

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Characterization of a Chiral-AGP capillary column coupled to a micro sample-enrichment system with UV and electrospray mass spectrometric detection

Cited by 14 publications

References 21 publications

Microcolumn liquid chromatography: instrumentation, detection and applications

Microcolumn liquid chromatography: instrumentation, detection and applications

Enantiomer separation of amino acids in immunoaffinity micro LC‐MS

Supramolecular systems‐based extraction‐separation techniques coupled to mass spectrometry

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