Characterization of a cellulase-free, neutral xylanase from CBS 288.54 and its biobleaching effect on wheat straw pulp

Li, X T; Jiang, Zhengqiang; Li, L T; Yang, Shaoqing; Feng, Weiyue; Fan, Jinxia; Kusakabe, Isao

doi:10.1016/j.biortech.2004.11.006

Cited by 88 publications

(57 citation statements)

References 27 publications

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“…Further, recovery of the purified enzyme from B. subtilis ASH following one step protocol was better than that obtained by using multistep protocol (1,5,16,24). However, grouping of two or more techniques resulted in higher purification fold (1,5,16,24,35) or recovery (7).…”

Section: Purification Of Xylanasementioning

confidence: 96%

“…Low molecular weight xylanases are preferred for commercial application in paper and pulp industry as they penetrate throughout the pulp fibres more effectively to hydrolyze xylan making the fiber structure more permeable to extraction of lignin by chemicals (21). (29) and Thermomyces lanuginosus (24). However, some xylanases were found to have lower K m ranging from 0.025-1.7 mg/ml (8,16,23,38).…”

Section: Molecular Weight Determinationmentioning

confidence: 97%

“…Other research groups reported purification of xylanase by employing a combination of two or more different methods including salt fractionation, ion-exchange, gel filtration and hydrophobic interaction chromatography (1,5,7,16,24,29,35,36). The overall purification in the present study using one step was higher than that reported from Bacillus amyloliquefaciens (7) and Bacillus circulans (36) using a multistep sequence of purification.…”

Section: Purification Of Xylanasementioning

confidence: 99%

“…However, grouping of two or more techniques resulted in higher purification fold (1,5,16,24,35) or recovery (7). …”

Section: Purification Of Xylanasementioning

confidence: 99%

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One-step purification and characterization of cellulase-free xylanase produced by alkalophilic Bacillus subtilis ash

Sanghi¹,

Garg²,

Gupta³

et al. 2010

Braz. J. Microbiol.

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The present study describes the one-step purification and characterization of an extracellular cellulase-free xylanase from a newly isolated alkalophilic and moderately thermophilic strain of Bacillus subtilis ASH.Xylanase was purified to homogeneity by 10.5-fold with ~43% recovery using ion-exchange chromatography through CM-Sephadex C-50. The purified enzyme revealed a single band on SDS-PAGE gel with a molecular mass of 23 kDa. It showed an optimum pH at 7.0 and was stable over the pH range 6.0-9.0. The optimum temperature for enzyme activity was 55 °C. The purified xylanase did not lose any activity up to 45 ºC, however, it retained 80% and 51% of its activity after pre-incubation at 55 ºC and 60 ºC, respectively. The enzyme obeyed Michaelis-Menton kinetics towards birch wood xylan with apparent K m 3.33 mg/ml and V max 100 IU/ml. The enzyme was strongly inhibited by Hg 2+ and Cu 2+ while enhanced by Co 2+ and Mn 2+. The purified enzyme could be stored at 4 ºC for six weeks without any loss of catalytic activity. The faster and economical purification of the cellulase-free xylanase from B. subtilis ASH by onestep procedure together with its appreciable stability at high temperature and alkaline pH makes it potentially effective for industrial applications.

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Section: Purification Of Xylanasementioning

confidence: 96%

Section: Molecular Weight Determinationmentioning

confidence: 97%

Section: Purification Of Xylanasementioning

confidence: 99%

“…However, grouping of two or more techniques resulted in higher purification fold (1,5,16,24,35) or recovery (7). …”

Section: Purification Of Xylanasementioning

confidence: 99%

See 2 more Smart Citations

One-step purification and characterization of cellulase-free xylanase produced by alkalophilic Bacillus subtilis ash

Sanghi¹,

Garg²,

Gupta³

et al. 2010

Braz. J. Microbiol.

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“…Viscosity reduction is not desirable because this property is related to the degree of cellulose polymerization and to paper strength (Paice et al 1988). Current studies on xylanase biobleaching have mainly focused on reduction of the consumption of bleaching chemicals (Garg et al 2011;Manimaran et al 2009;Khandeparkar and Bhosle 2007;Li et al 2005); very little attention has been given to the effects of xylanase on the yield and viscosity of pulp (Kantelinen et al 1993;Xu et al 2013). In the present study, a fermentation of S. griseorubens LH-3 was conducted, the crude xylanase extract was prepared, and the enzymatic properties of the crude xylanase were studied.…”

Section: Introductionmentioning

confidence: 99%

Biobleaching Effects of Crude Xylanase from Streptomyces griseorubens LH-3 on Eucalyptus Kraft Pulp

Cheng¹,

Chen²,

Huang³

et al. 2013

BioResources

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In this work, a xylanase-producing strain, Streptomyces griseorubens LH-3, was cultured, and the crude xylanase was prepared. Analysis of its enzymatic properties revealed that the crude xylanase possessed good thermal stability at temperatures below 60 o C, exhibited a wide pH range from 4.0 to 9.0, and was cellulase-free. This crude enzyme was used to treat eucalyptus kraft pulp, and the release of chromophores was the highest at the dosage of 20 IU g −1 dry pulp. Compared with the untreated group, biobleaching of eucalyptus kraft pulp with this enzyme increased the brightness of the pulp by 12.9% and reduced the Kappa number by 27.4%. Biobleaching of eucalyptus kraft pulp with this enzyme obtained the same final pulp brightness compared with that of the control; however, hydrogen peroxide consumption was reduced by 17% and the yield and viscosity of the pulp was increased by 1.47% and 1.53%, respectively. This crude xylanase has promising potential for industrial applications.

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Endo-β-1,4-xylanase: An Overview of Recent Developments

Rodrigues

2016

Biofuel and Biorefinery Technologies

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Characterization of a cellulase-free, neutral xylanase from CBS 288.54 and its biobleaching effect on wheat straw pulp

Cited by 88 publications

References 27 publications

One-step purification and characterization of cellulase-free xylanase produced by alkalophilic Bacillus subtilis ash

One-step purification and characterization of cellulase-free xylanase produced by alkalophilic Bacillus subtilis ash

Biobleaching Effects of Crude Xylanase from Streptomyces griseorubens LH-3 on Eucalyptus Kraft Pulp

Endo-β-1,4-xylanase: An Overview of Recent Developments

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