Characterization of a birch (Betula pendula Roth.) embryogenic gene, BP8

Puupponen‐Pimiä, Riitta; Saloheimo, Markku; Vasara, Tuija; Ra, Runar; Gaugecz, Janka; Kurten, Ulrika; Knowles, Jonathan; Keränen, Sirkka; Kauppinen, Veli

doi:10.1007/bf00029018

Cited by 23 publications

(17 citation statements)

References 16 publications

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“…An exception is the 6th best hit, a late embryogenesis abundant (LEA) protein BP8, from birch (Puupponen-Pimiä et al, 1993). This is a group 3 LEA protein (Pfam LEA_4 family Finn et al, 2008), which is distinct from several other LEA classes.…”

Section: Results Of the Coiled Coil/fourier Transform/composition-basmentioning

confidence: 99%

A novel coiled-coil repeat variant in a class of bacterial cytoskeletal proteins

Walshaw

Gillespie

Kelemen

2010

Journal of Structural Biology

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a b s t r a c tIn recent years, a number of bacterial coiled-coil proteins have been characterised which have roles in cell growth and morphology. Several have been shown to have a cytoskeletal function and some have been proposed to have an IF-like character in particular. We recently demonstrated in Streptomyces coelicolor a cytoskeletal role of Scy, a large protein implicated in filamentous growth, whose sequence is dominated by an unusual coiled-coil repeat. We present a detailed analysis of this 51-residue repeat and conclude that it is likely to form a parallel dimeric non-canonical coiled coil based on hendecads but with regions of local underwinding reflecting highly periodic modifications in the sequence. We also demonstrate that traditional sequence similarity searching is insufficient to identify all but the close orthologues of such repeat-dominated proteins, but that by an analysis of repeat periodicity and composition, remote homologues can be found. One clear candidate, despite a great size discrepancy and unremarkable sequence identity, is the known filament-former FilP in the same species. Both proteins appear distinct from the archetypal bacterial IF-like protein; they therefore may constitute a new class of bacterial filamentous protein. The similar sequence characteristics of both suggest their likely oligomer state and a possible mechanism for higher-order assembly into filaments. Another remote homologue in Actinomyces was highlighted by this method. Further, a known coiled-coil protein, DivIVA, appears to share some of these sequence characteristics.

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Section: Results Of the Coiled Coil/fourier Transform/composition-basmentioning

confidence: 99%

A novel coiled-coil repeat variant in a class of bacterial cytoskeletal proteins

Walshaw

Gillespie

Kelemen

2010

Journal of Structural Biology

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“…This is supported by our other recent studies of ozone-stressed and droughtstressed birch clones (E. Pa$ a$ kko$ nen et al, unpublished) indicating high phenotypic plasticity of leaves and good ability for structural acclimation to reduce water loss, appearing for example as thickened cell walls and smaller leaf size. Extremely low expression level of the BP8 gene during the desiccation phase in the embryogenesis in birch cell cultures has also been reported (Puupponen-Pimia$ et al, 1993).Differential ozone sensitivity of birch clones has previously been reported in several studies by Pa$ a$ kko$ nen et al (1993, 1996, 1997 a). As in the earlier experiments, ozone sensitivity of clone 5 in the present study was indicated by ozone-induced growth reductions, a considerable proportion of visibly injured and yellowed leaves and high induction of genes for PAL and PR-10, occurring the lower stomatal conductance and probable lower ozone uptake by WW ozone plants than by the lessozone-sensitive clone 2.…”

mentioning

confidence: 65%

Section: mentioning

confidence: 85%

“…A single ozone pulse (150 nl l −" ) resulted in rapid and transient increase in Pal transcript (Tuomainen et al, 1996) in birch clones the same as those used in the current experiment. Ozone induction of PAL has also been reported in pine (Pinus sylvestris) (Rosemann, Heller & Sandermann 1991) and parsley (Eckey-Kaltenbach et al, 1994).Late embryogenesis abundant (LEA) proteins are synthesized during seed maturation and during drought and low-temperature stress in seeds and vegetative tissues (Bray, 1993 ;Puupponen-Pimia$ et al, 1993 ;Marttila, Tenhola & Mikkonen, 1996). LEA proteins have high water-binding capacity and protect cells during desiccation stress by stabilizing cellular structures, sequestering ions and replacing water molecules (Bray, 1993).…”

mentioning

confidence: 97%

“…LEA proteins have high water-binding capacity and protect cells during desiccation stress by stabilizing cellular structures, sequestering ions and replacing water molecules (Bray, 1993). The birch gene BP8, isolated from embryogenic tissue culture by Puupponen-Pimia$ et al (1993), encodes a protein belonging to the LEA group of proteins and thus is a potential marker for drought stress in birch leaves.Additional studies are needed to establish the relationship of biochemical defence systems to ozone sensitivity. In this paper we aim to relate the Ozone-and drought-induced stress proteins in birch 297 responses of specific stress-related proteins with growth rates, appearance of visible leaf injuries and stomatal conductance in an ozone-sensitive and in a tolerant (less sensitive) birch clone exposed to ozone and drought.…”

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confidence: 99%

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Induction of genes for the stress proteins PR‐10 and PAL in relation to growth, visible injuries and stomatal conductance in birch (Betula pendula) clones exposed to ozone and/or drought

et al. 1998

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Well watered (WW) or drought-stressed (DS) saplings of ozone-sensitive and ozone-tolerant (less sensitive) birch (Betula pendula Roth) clones were exposed for 43 d to 0 nl l −" or 100 nl l −" ozone. Relative growth rates of leaves, stem, and roots, leaf discolouration, stomatal conductance and induction of genes encoding stress-related proteins PR-10, PAL and a LEA-group protein BP8 were determined. In general, both ozone and drought stress, singly and in combination, increased transcript levels of PR-10 in both clones. This was related to lower induction of PAL (except in older leaves of the tolerant clone), and increased proportions of visibly injured and yellowed leaves in ozone-exposed plants. The clones differed in their stomatal conductance and growth responses. In the less sensitive clone 2, ozone did not affect growth rates, but high stomatal conductance was observed in WW ozoneexposed plants. The more sensitive clone 5 showed, on the contrary, reduced growth rates and low stomatal conductance in WW ozone plants. Interestingly, clone 2 was sensitive to drought stress alone, whereas clone 5 was highly sensitive to ozone and drought stress experienced together. The results show that appearance of visible injuries (necrotic flecks) and enhanced yellowing of leaves coincided with the induction of genes for stress proteins PR-10 and PAL. The short-term growth responses, however, seemed to be separate processes. Additionally, stomatal conductance was related to leaf injuries and growth rates in a complicated manner, emphasizing the complex nature of ozone sensitivity\tolerance mechanisms in birch.Key words : Betula pendula Roth (birch), clones, ozone, drought, stress proteins. Forest trees grow in a dynamic environment and are continuously exposed to several abiotic and biotic stress factors (Lefohn, 1992). Atmospheric ozone and drought are potential stress factors for forest trees in the USA (e.g. McLaughlin & Downing, 1996) and Europe including Scandinavia (e.g. Pearson & Mansfield, 1993 ;Karlsson et al., 1995 ; * To whom correspondence should be addressed. E-mail : Elina.Paakkonen!messi.uku.fi Pa$ a$ kko$ nen, 1996 ; Pa$ a$ kko$ nen, Holopainen & Ka$ renlampi, 1997 a). Physiological, structural, and biochemical responses of birch to ozone have recently been studied in several open-field and chamber experiments. Typical ozone responses in ozonesensitive birch clones were impaired shoot growth, accelerated leaf senescence, visible leaf injuries, ultrastructural chloroplast injuries, increased stomatal density and lower stomatal conductance (Pa$ a$ kko$ nen et al., 1993(Pa$ a$ kko$ nen et al., a,b, 1996 a ; Pa$ a$ kko$ nen, 1996). Lower net photosynthesis and decreased Rubisco and chlorophyll contents have 296 E. PaW aW kkoW nen and others also been observed during open-field fumigations . In the chamber exposures, on the other hand, increased activities of superoxide dismutase, peroxidase and glutathione reductase, induction of PAL (phenylalanine ammonia-lyase), accumulation of free pu...

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slowmo is required for Drosophila germline proliferation

Reeve

Çarhan

Dee

et al. 2007

Genesis

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Null mutations in the Drosophila gene, slowmo (slmo), result in reduced mobility and lethality in first-instar larvae. Slowmo encodes a mitochondrial protein of unknown function, as do the two other homologs found in Drosophila. Here, we have studied a hypomorphic P-element allele of slmo demonstrating its effects on germline divisions in both testes and ovaries. Using in situ studies, enhancer-trap activity, and promoter fusions, we have shown that slmo expression in testes is found in the somatic cyst cells (SCC). The hypomorphic allele for Slmo revealed apoptotic loss of germline cells in the larval germline, culminating in a complete absence of the germline in adult flies. In females, a similar degeneration of the germarium is observed, while reporter gene expression is found in both germline and somatic cells. Using a null mutation in female germline clones, we find slmo is dispensable from the germline cells. Our results suggest that Slowmo is not required in germline cells directly, but is required in SCCs responsible for maintaining germline survival in both sexes.

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Characterization of a birch (Betula pendula Roth.) embryogenic gene, BP8

Cited by 23 publications

References 16 publications

A novel coiled-coil repeat variant in a class of bacterial cytoskeletal proteins

A novel coiled-coil repeat variant in a class of bacterial cytoskeletal proteins

Induction of genes for the stress proteins PR‐10 and PAL in relation to growth, visible injuries and stomatal conductance in birch (Betula pendula) clones exposed to ozone and/or drought

slowmo is required for Drosophila germline proliferation

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