Characterization of a bioactive fiber scaffold with entrapped HUVECs in coaxial electrospun core-shell fiber

Ang, Hui Ying; Irvine, Scott Alexander; Avrahami, Ron; Sarig, Udi; Bronshtein, Tomer; Zussman, Eyal; Boey, Freddy Yin Chiang; Machluf, Marcelle; Venkatraman, Subbu

doi:10.4161/biom.28238

Cited by 17 publications

(13 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…FDA is a substrate for cell permeable esterase that evaluates the enzymatic activity and cell membrane integrity 28 . NIH 3T3 fibroblast cell proliferation and attachment on the hydrogel samples were observed by FDA staining.…”

Section: Resultsmentioning

confidence: 99%

Accelerated Healing of Diabetic Wounds Treated with L-Glutamic acid Loaded Hydrogels Through Enhanced Collagen Deposition and Angiogenesis: An In Vivo Study

Ponrasu

Balaji

Chakraborty

et al. 2017

Sci Rep

View full text Add to dashboard Cite

We have developed L-glutamic acid (LG) loaded chitosan (CS) hydrogels to treat diabetic wounds. Although literature reports wound healing effects of poly(glutamic acid)-based materials, there are no studies on the potential of L-glutamic acid in treating diabetic wounds. As LG is a direct precursor for proline synthesis, which is crucial for collagen synthesis, we have prepared CS + LG hydrogels to accelerate diabetic wound healing. Physiochemical properties of the CS + LG hydrogels showed good swelling, thermal stability, smooth surface morphology, and controlled biodegradation. The addition of LG to CS hydrogels did not alter their biocompatibility significantly. CS + LG hydrogel treatment showed rapid wound contraction compared to control and chitosan hydrogel. Period of epithelialization is significantly reduced in CS + LG hydrogel treated wounds (16 days) compared to CS hydrogel (20 days), and control (26 days). Collagen synthesis and crosslinking are also significantly improved in CS + LG hydrogel treated diabetic rats. Histopathology and immunohistochemistry results revealed that the CS + LG hydrogel dressing accelerated vascularization and macrophage recruitment to enhance diabetic wound healing. These results demonstrate that incorporation of LG can improve collagen deposition, and vascularization, and aid in faster tissue regeneration. Therefore, CS + LG hydrogels could be an effective wound dressing used to treat diabetic wounds.

show abstract

Section: Resultsmentioning

confidence: 99%

Accelerated Healing of Diabetic Wounds Treated with L-Glutamic acid Loaded Hydrogels Through Enhanced Collagen Deposition and Angiogenesis: An In Vivo Study

Ponrasu

Balaji

Chakraborty

et al. 2017

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Furthermore, there is a recognized potential for cells to be incorporated into the fabrication process, as a “bottoms-up” approach with the advantage of manufacturing a construct with an even distribution of viable cells. Techniques such as electrospinning have previously been developed to deliver cells directly into a construct (Ang et al 2014 ), however this technique suffers from a lack of deposition control due to the randomness in electrospun fiber grounding. FFF offers the potential of precision fabrication using cell bearing material under computer control.…”

Section: Introductionmentioning

confidence: 99%

Printing cell-laden gelatin constructs by free-form fabrication and enzymatic protein crosslinking

Irvine

Agrawal

Lee

et al. 2015

Biomed Microdevices

Self Cite

115

102

View full text Add to dashboard Cite

Considerable interest has arisen in precision fabrication of cell bearing scaffolds and structures by free form fabrication. Gelatin is an ideal material for creating cell entrapping constructs, yet its application in free form fabrication remains challenging. We demonstrate the use of gelatin, crosslinked with microbial transglutaminase (mTgase), as a material to print cell bearing hydrogels for both 2-dimensional (2-D) precision patterns and 3-dimensional (3-D) constructs. The precision patterning was attained with 3 % gelatin and 2 % high molecular weight poly (ethylene oxide) (PEO) whereas 3-D constructs were obtained using a 5 % gelatin solution. These hydrogels, referred to as “bioinks” supported entrapped cell growth, allowing cell spreading and proliferation for both HEK293 cells and Human Umbilical Vein Endothelial Cells (HUVECs). These bioinks were shown to be dispensable by robotic precision, forming patterns and constructs that were insoluble and of suitable stiffness to endure post gelation handling. The two bioinks were further characterized for fabrication parameters and mechanical properties.Electronic supplementary materialThe online version of this article (doi:10.1007/s10544-014-9915-8) contains supplementary material, which is available to authorized users.

show abstract

“…encapsulated platelet‐derived growth factor‐BB (PDGF‐BB) in PCL fibers via coaxial electrospinning, and tested the cell proliferation to ensure the bioactivity. Also Ang et al . entrapped endothelial cells (HUVECs) of the human umbilical vein in electrospun poly(ethylene oxide) (PEO)/PCL C/S nanofibers.…”

Section: C/s Nanofibersmentioning

confidence: 99%

Recent advances in core/shell bicomponent fibers and nanofibers: A review

Naeimirad

Ali

Kotek

et al. 2018

J of Applied Polymer Sci

140

103

View full text Add to dashboard Cite

There has been a steady progress in developing synthetic fibers in the past few years. Bicomponent fibers and nanofibers in a core/shell (C/S) configuration, including two dissimilar materials have presented unusual potential for use in many novel applications. These fibers can be produced using a variety of materials via different techniques i.e., coaxial melt spinning and electrospinning. In this review, we discuss the recent advances in C/S fibers and nanofibers' production. The first part has been assigned to the bicomponent fibers manufacturing technology, while production and applications of C/S nanofibers have been described in the second part.

show abstract

Characterization of a bioactive fiber scaffold with entrapped HUVECs in coaxial electrospun core-shell fiber

Cited by 17 publications

References 33 publications

Accelerated Healing of Diabetic Wounds Treated with L-Glutamic acid Loaded Hydrogels Through Enhanced Collagen Deposition and Angiogenesis: An In Vivo Study

Accelerated Healing of Diabetic Wounds Treated with L-Glutamic acid Loaded Hydrogels Through Enhanced Collagen Deposition and Angiogenesis: An In Vivo Study

Printing cell-laden gelatin constructs by free-form fabrication and enzymatic protein crosslinking

Recent advances in core/shell bicomponent fibers and nanofibers: A review

Contact Info

Product

Resources

About