Characterization of a bacteriocin produced by Streptococcus thermophilus 81

Иванова, И.; Miteva, Vanya; Stefanova, Ts; Pantev, A.; Budakov, Ivo; Danova, Svetla; Moncheva, Penka; Nikolova, Ivelina; Dousset, Xavier; Boyaval, Patrick

doi:10.1016/s0168-1605(98)00067-1

Cited by 125 publications

(98 citation statements)

References 17 publications

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“…The procedure of Ivanova et al (1998) was used. The cells of a 200 ml 18 h culture were collected and washed in 10 mM Tris-HCl, pH 8.2 and resuspended in 5 ml 20 mM Tris-HCl, pH 8.2.…”

Section: Plasmid Isolationmentioning

confidence: 99%

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Detection, partial purification and characterization of bacteriocin produced by Lactobacillus brevis FPTLB3 isolated from freshwater fish

2011

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Lactobacillus brevis FPTLB3 was isolated from freshwater fish, capable of producing bacteriocin that had broad spectrum of inhibition (3200 AU/ml) against Escherichia coli MTCC 1563, Enterococcus faecalis MTCC 2729, Lactobacillus casei MTCC 1423, Lactobacillus sakei ATCC 15521 and Staphylococcus aureus ATCC 25923. The antimicrobial activity of crude supernatant fluid was stable after heating at 121°C for 60 min and declined thereafter. Stability of antimicrobial activity was observed at pH range of 2.0 to 8.0.

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“…The procedure of Ivanova et al (1998) was used. The cells of a 200 ml 18 h culture were collected and washed in 10 mM Tris-HCl, pH 8.2 and resuspended in 5 ml 20 mM Tris-HCl, pH 8.2.…”

Section: Plasmid Isolationmentioning

confidence: 99%

“…In most cases the genes encoding bacteriocins are harbored on plasmids (Huot et al 1996). However, for a number of bacteriocins the genes have been located on the genome (Ivanova et al 1998;Todorov et al 1999). Since no plasmid was isolated from the strain Lb.…”

Section: Plasmid Isolationmentioning

confidence: 99%

Detection, partial purification and characterization of bacteriocin produced by Lactobacillus brevis FPTLB3 isolated from freshwater fish

2011

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“…Several strains of S. thermophilus have been shown to naturally produce bacteriocins that are small, ribosomally produced peptides with narrow and/or broad spectrum antimicrobial activity (Villani et al, 1995;Ward & Somkuti, 1995;Marciset et al, 1997;Aktypis et al, 1998;Ivanova et al, 1998;Mathot et al, 2003;Gilbreth & Somkuti, 2005;Fontaine & Hols, 2008). Genome sequencing of Streptococcus thermophilus strains LMD-9, CNRZ1066 and LMG18311 revealed a cluster of genes encoding potential bacteriocin-like peptides (blp) based on genetic similarities to the class II bacteriocin locus described for S. pneumoniae (deSaizieu et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Effect of a BlpC-Based Quorum-Sensing Induction Peptide on Bacteriocin Production in Streptococcus thermophilus

Somkuti¹,

Renye²

2014

JFR

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Bacteriocin synthesis in some Streptococcus thermophilus strains is under the control of a complex blp locus but bacteriocin is produced only when a quorum-sensing regulatory mechanism is activated by the protein product of the blpC component. To demonstrate the regulatory effect of BlpC in S. thermophilus ST110 (NRRL-B59671), which naturally produces bacteriocin, the effect of the 30mer quorum-sensing induction peptide (QSIP) embedded in BlpC was tested in a knockout mutant in which the blpC gene was eliminated and was devoid of antimicrobial activity. Between concentrations of 30 and 250 ng/ml, the addition of synthetic QSIP to cultures at several points of the growth curve resulted in the accumulation of up to 3,200 units/ml of bacteriocin after 8 h of growth at 37 ºC. Addition of QSIP to the culture in late log phase (OD 660 ≥ 1.0) when the medium pH is already 4.8 or lower, failed to trigger bacteriocin production. We used synthetic QSIP to survey its impact on 35 strains of S. thermophilus that do not display bacteriocin activity by agar diffusion assays. The addition of QSIP (250 ng/ml) to S. thermophilus cultures in the early or mid-log phase induced bacteriocin production in two strains that could be re-classified as bacteriocin producers. The results demonstrated the involvement of a quorum-sensing regulatory mechanism in bacteriocin synthesis in S. thermophilus ST110 and also demonstrated the utility of the 30 mer QSIP in discovering bacteriocins with potentially novel antimicrobial spectra by enhancing bacteriocin production in other strains of S. thermophilus that ordinarily display a bacteriocin-negative phenotype.

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“…However, inhibitory effects of nisin (Cutter and Siragusa, 1995), bacteriocin produced by Lactobacillus paracasei subsp. paracasei (Caridi, 2002), bacteriocin ST151BR produced by Lactobacillus pentosus ST151BR (Todorov and Dicks, 2004), thermophylin produced by Streptococcus thermophillus (Ivanova et al, 1998) and some enterocins (Gálvez et al, 1989;Simonetta et al, 1997;Jennes et al, 1999) on Gram-negative bacteria through their synergetic effects with other antimicrobials has gained increased interest (Helander et al, 1997). Treatment of the neutralized supernatants with catalase did not change their activities, indicating that the inhibition recorded was not acid and hydrogen peroxide.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial activities of the bacteriocin-like substances produced by lactic acid bacteria isolated from Moroccan dromedary milk

Khay¹,

Idaomar²,

Castro³

et al. 2011

Afr. J. Biotechnol.

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A total of 450 different colonies, isolated from 25 samples of dromedary milk collected from Laâyoune region of Morocco, were tested for antimicrobial compounds production. Out of these, 30 were determined to be lactic acid bacteria (LAB) and able to inhibit the growth of the indicator strain Listeria innocua CECT 4030. Seven isolates were selected by the large and clear zones of inhibition when tested by the agar well diffusion assay. They were classified by phenotypic and biochemical analysis as two Enterococcus durans (E204 and E214), two Lactococcus lactis (R75 and R76), one Enterococcus faecium R111, one Lactococcus cremoris R112 and one Enterococcus avium R122. Their antimicrobial compounds were detected in cell-free culture supernatant fluids under conditions that eliminate acid and hydrogen peroxide inhibition. The antimicrobial activity was altered after treatment with trypsin, -chymotrypsin, pepsin or papain which confirms the proteinaceous nature of the inhibition. It was heat stable even at autoclaving temperature (121°C for 15 min) and also active over a wide pH range (2 to 10). This fact suggests that bacteriocin-like produced by the seven LAB strains may find application as biopreservatives in food products.

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Characterization of a bacteriocin produced by Streptococcus thermophilus 81

Cited by 125 publications

References 17 publications

Detection, partial purification and characterization of bacteriocin produced by Lactobacillus brevis FPTLB3 isolated from freshwater fish

Detection, partial purification and characterization of bacteriocin produced by Lactobacillus brevis FPTLB3 isolated from freshwater fish

Effect of a BlpC-Based Quorum-Sensing Induction Peptide on Bacteriocin Production in Streptococcus thermophilus

Antimicrobial activities of the bacteriocin-like substances produced by lactic acid bacteria isolated from Moroccan dromedary milk

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