Characterization of a Bacillus velezensis with Antibacterial Activity and Its Inhibitory Effect on Gray Mold Germ

Abstract

The present study provides a comprehensive overview of the Bacillus velezensis strain Htq6, and its potential applications in plant disease control. Htq6 is an endophytic bacterium derived from walnut, which was found to possess a strong inhibitory effect on a wide range of plant pathogenic microorganisms and was identified as a good plant disease control agent. The entire genome of the Bacillus velezensis Htq6 was sequenced, and a comparative genomic analysis was conducted with various Bacillus species in order to better understand the mechanism of the strain’s biological control. At the same time, a new classification result was presented. Additionally, transcriptome analysis was performed to explore the response mechanism of tomato gray mold fungus after treatment with the fermentation liquid of Bacillus velezensis Htq6. The study analyzed the distribution of various secondary metabolite gene clusters in the Bacillus model strains and employed RNA-Seq technology to obtain transcriptome expression profiles. Furthermore, the cell wall, cell membrane, and antioxidant-related genes of Botrytis cinerea were analyzed, providing insight into the antibacterial mechanism of biocontrol bacteria and the stress response mechanism of Botrytis cinerea. The results of the research are promising, and could potentially lead to the development of an effective biocontrol agent for the prevention and control of various plant diseases.

Optimization of Antioxidant Activity of Compounds Generated during Ginseng Extract Fermentation Supplemented with Lactobacillus

“…Li et al [ 30 ] found that LAB can produce β-glucosidase during the metabolic process, and the magnitude of its activity is the main factor determining the conversion of saponins into rare saponins. β-Glucosidase can also utilize substrates to generate other active substances, thereby enhancing the antioxidant activity of the fermentation broth [ 31 ].…”

“…Li et al [30] found that LAB can produce β-glucosidase during the metabolic process, and the magnitude of its activity is the main factor determining the conversion of saponins Li et al [30] found that LAB can produce β-glucosidase during the metabolic process, and the magnitude of its activity is the main factor determining the conversion of saponins into rare saponins. β-Glucosidase can also utilize substrates to generate other active substances, thereby enhancing the antioxidant activity of the fermentation broth [31].…”

“…The liquid chromatography-mass spectrometry ion source operating parameters were as follows: Electrospray ion source (ESI) was used, the scanning mode was positive and negative ion switching, the multiple reaction monitoring mode was used for detection, the atomized gas flow was 3.0 L/min, the drying gas flow was 10.0 L/min, the heating gas flow was 10 L/min, the connection temperature was 300 • C, the desolvation line was at 250 • C, the heating block temperature was 400 • C, and the collision-induced dissociation pressure was 270 kPa. The following three factors were varied with the aim of increasing the antioxidant activity of the fermentation products: fermentation time (15,30,45,60, and 75 h), fermentation temperature (26,28,30,32, and 34 • C), and inoculum size (1.5, 2.0, 2.5, 3.0, and 3.5% v/v). The selection of time, temperature, and inoculum size as the main factors for process optimization was based on the method proposed by Tao et al [75].…”

Draft genome sequence of Bacillus velezensis strains AOA1 and AKS2, the potential plant growth-promoting rhizobacteria

“…Many species exhibit a wide range of physiological traits, which enable their survival in a diverse natural environment ( 1 ). Bacillus velezensis was formerly classified as strains of Bacillus amyloliquefaciens but reclassified based on genomic analysis ( 2 , 3 ). Comparative genomics and DNA-DNA relatedness calculations had, however, showed that B. velezensis was taxonomically distinct from B. amyloliquefaciens ( 4 ).…”

Preparation of Azoxystrobin-Zinc Metal–Organic Framework/Biomass Charcoal Composite Materials and Application in the Prevention and Control of Gray Mold in Tomato

“…The prevalence of vegetable fungal diseases is on the rise, and gray mold of tomato caused by Botrytis cinerea is a serious threat to crop yield and quality [2,3]. Botrytis cinerea causes yield loss of more than 20% and economic loss of tens of billions of yuan each year [4]. It poses a significant threat to the productivity of tomato crops by infiltrating multiple plant components, including stems, leaves, and fruits, thereby compromising food safety [5].…”