Characterization of a 5HS-7DS.7DL wheat-barley translocation line and physical mapping of the 7D chromosome using SSR markers

Abstract

A spontaneous wheat-barley translocation line was previously detected in the progenies of the Mv9kr1 × 'Igri' wheat-barley hybrid and the translocation was identified as 5HS-7DS.7DL. Multicolor genomic in situ hybridization (mcGISH) with D and H genomic DNA probes and three-color fluorescence in situ hybridization (FISH) with repetitive DNA probes (Afa-family, pSc119.2, and pTa71) were performed to characterize the rearranged chromosome. The effect of 5HS and the deleted 7DS fragment on the morphological traits (plant height, fertility, yield, and spike characteristics) of wheat was assessed. Despite the non-compensating nature of the translocation, the plants showed good viability. The aim of the study was to physically localize SSR markers to the telomeric and subtelomeric regions of the 7DS chromosome arm. Of the 45 microsatellite markers analyzed, ten (Xbarc0184, Xwmc0506, Xgdm0130, Xgwm0735, Xgwm1258, Xgwm1123, Xgwm1250, Xgwm1055, Xgwm1220, and Xgwm0635) failed to amplify any 7DS-specific fragments, signaling the elimination of a short chromosome segment in the telomeric region. The breakpoint of the 5HS-7DS.7DL translocation appeared to be more distal than that of reported deletion lines, which provides a new physical landmark for future deletion mapping studies.

Wheat–barley hybridization: the last 40 years

“…Sepsi et al (2006) produced wheat/barley translocations as the result of induced homoeologous chromosome pairing in a 4H(4D) wheat-barley substitution line by crossing with the line CO4-1, which carries the Ph suppressor gene from Aegilops speltoides. Kruppa et al (2013) reported the development of a 4HL.5DL Robertsonian translocation line after crossing the 4H(4D) wheatbarley substitution line with the CSph1b mutant. The rearrangement was confirmed with sequential GISH, FISH and SSR markers.…”

“…This translocation line carries a 4BS wheat chromosome arm and a 7HL chromosome arm from the Ukrainian six-rowed winter barley. Another spontaneous wheat/ barley translocation line was identified as 5HS-7DS.7DL in the progenies of the Mv9kr1 9 Igri wheat-barley hybrid (Kruppa et al 2013). Despite the non-compensating nature of the translocation, the plants showed good viability.…”

Peer Review #2 of "Complex characterization of oat (Avena sativa L.) lines obtained by wide crossing with maize (Zea mays L.) (v0.1)"

“…This technique allows for the genome-specific chromosome labeling in the cells of allopolyploid or hybrid species. It has been widely used in plant studies to identify putative parental genomes in the interspecific or intergeneric hybrids or allopolyploids (for the review see Chester et al 2010;Silva and Souza, 2013), to study the evolution of polyploid genomes (Song et al 1995;Gaeta et al 2007;Majka et al 2018;Tan et al 2017), or to detect the introgression of alien chromatin (Schneider et al 2005;Tan et al 2005) Manuscript to be reviewed as translocation (Kruppa et al 2013), addition (Molnar-Lang et al 2000;Ji and Chetelat 2007), or substitution (Pan et al 2017) lines.…”

Genomics of Wild Relatives and Alien Introgressions

“…Genomic in situ hybridization (GISH) uses genomic DNA as a probe (Schwarzacher et al 1989) and permits determination of genomic constitution of allopolyploid Triticeae, and to detect alien chromatin introgressed into wheat. Combined with chromosome banding and/or FISH, the method allows location and identification of wheat-alien translocation breakpoints (Friebe et al 1992(Friebe et al , 1993Jiang et al 1993;Molnár-Láng et al 2000Liu et al 2010;Kruppa et al 2013). While cytogenetic methods are irreplaceable to verify genomic constitution in interspecific hybrids, the limited sensitivity and spatial resolution, and especially their laborious and time consuming nature seriously limit their suitability for large scale selection of wheat-alien introgressions.…”