Characterization of 58 STRs and 94 SNPs with the ForenSeq™ DNA Signature Prep Kit in Mexican-Mestizos from the Monterrey City (Northeast, Mexico)

Abstract

Background. STR allele frequency databases from populations are necessary to take full advantage of the increased power of discrimination offered by massive parallel sequencing (MPS) platforms.Material and methods. For this reason, we sequenced 58 STRs (aSTRs, X-STRs, and Y-STRs) and 94 identity informative SNPs (iiSNPs) on 105 Mestizo (admixed) individuals from Monterrey City (Northeast, Mexico), with the Primer Set-A of the ForenSeqTM DNA Signature Prep Kit. Results. Most of the STR markers were in Hardy Weinberg equilibrium, with a few exceptions. We found 346 different length-based alleles for these 58 STRs; nevertheless, they became 528 alleles when the sequence was assessed. The combined power of discrimination from autosomal STRs (aSTRs) was –virtually– 100 % in both length and sequence-based alleles, while the power of exclusion were 99.9999999976065 and 99.9999999999494%, respectively. Haplotypes based on X-STRs and Y-STRs showed 100% of discriminatory capacity. Conclusion. These results provide –for the first time– forensic genomic population data from Mexico necessary for interpretation in kinship and criminal analyses.