Characterization of [3H]CGP 12177 Binding to β-Adrenergic Receptors in Intact Eel Hepatocytes

Fabbri, Elena; Selva, Corrado; Moon, Thomas W.; Capuzzo, Antonio

doi:10.1006/gcen.2000.7591

Cited by 10 publications

(7 citation statements)

References 17 publications

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“…To determine the distribution pattern of β‐ARs and compare it with the immunohistochemical labeling of β 2 ‐AR in the adult zebrafish brain, quantitative autoradiographic experiments were performed using [ 3 H]CGP‐12177, a selective antagonist (Fernández‐López et al,1997). [ 3 H]CGP‐12177 binds equipotently to β 1 ‐ and β 2 ‐adrenoceptor subtypes (Revilla et al,1998a) in many vertebrate species, such as mammals (Morin et al,1992), birds (Revilla et al,1998a,2000), reptiles (Fabbri et al,1997), amphibians (Fabbri et al,1997), and bony fish (Niina et al,1988; Perry and Reid,1992; Gamperl et al,1994; Jozefowski and Plytyez,1998; Fabbri et al,2001).…”

Section: Methodsmentioning

confidence: 99%

Regional distribution and cellular localization of β₂‐adrenoceptors in the adult zebrafish brain (Danio rerio)

Ampatzis

Dermon

2010

J of Comparative Neurology

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The beta(2)-adrenergic receptors (ARs) are G-protein-coupled receptors that mediate the physiological responses to adrenaline and noradrenaline. The present study aimed to determine the regional distribution of beta(2)-ARs in the adult zebrafish (Danio rerio) brain by means of in vitro autoradiographic and immunohistochemical methods. The immunohistochemical localization of beta(2)-ARs, in agreement with the quantitative beta-adrenoceptor autoradiography, showed a wide distribution of beta(2)-ARs in the adult zebrafish brain. The cerebellum and the dorsal zone of periventricular hypothalamus exhibited the highest density of [(3)H]CGP-12177 binding sites and beta(2)-AR immunoreactivity. Neuronal cells strongly stained for beta(2)-ARs were found in the periventricular ventral telencephalic area, magnocellular and parvocellular superficial pretectal nuclei (PSm, PSp), occulomotor nucleus (NIII), locus coeruleus (LC), medial octavolateral nucleus (MON), magnocellular octaval nucleus (MaON) reticular formation (SRF, IMRF, IRF), and ganglionic cell layer of cerebellum. Interestingly, in most cases (NIII, LC, MON, MaON, SRF, IMRF, ganglionic cerebellar layer) beta(2)-ARs were colocalized with alpha(2A)-ARs in the same neuron, suggesting their interaction for mediating the physiological functions of nor/adrenaline. Moderate to low labeling of beta(2)-ARs was found in neurons in dorsal telencephalic area, optic tectum (TeO), torus semicircularis (TS), and periventricular gray zone of optic tectum (PGZ). In addition to neuronal, glial expression of beta(2)-ARs was found in astrocytic fibers located in the central gray and dorsal rhombencephalic midline, in close relation to the ventricle. The autoradiographic and immunohistochemical distribution pattern of beta(2)-ARs in the adult zebrafish brain further support the conserved profile of adrenergic/noradrenergic system through vertebrate brain evolution.

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Section: Methodsmentioning

confidence: 99%

Regional distribution and cellular localization of β₂‐adrenoceptors in the adult zebrafish brain (Danio rerio)

Ampatzis

Dermon

2010

J of Comparative Neurology

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“…Also in eel Anguilla anguilla (Fabbri et al, 2001) and trout there was only one β-adrenoceptor type present in the liver (Fabbri et al, 1995;Dugan and Moon, 1998); in trout it was identified as a β 2 -adrenoceptor McKinley and Hazel, 1993). In rockfish Sebastes caurinus hepatocytes, glucose release was mediated by the β 1 -adrenoceptor although the presence of another adrenoceptor could not be excluded (Danulat and Mommsen, 1990).…”

Section: Glucosementioning

confidence: 99%

Beta-adrenergic control of plasma glucose and free fatty acid levels in the air-breathing African catfishClarias gariepinusBurchell 1822

Heeswijk

Vianen

Thillart

et al. 2005

Journal of Experimental Biology

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In several water-breathing fish species, β-adrenergic receptor stimulation by noradrenaline leads to a decrease in plasma free fatty acid (FFA) levels, as opposed to an increase in air-breathing mammals. We hypothesised that this change in adrenergic control is related to the mode of breathing. Therefore, cannulated air-breathing African catfish were infused for 90·min with noradrenaline or with the nonselective β-agonist, isoprenaline. To identify the receptor type involved, a bolus of either a selective β 1 -antagonist (atenolol) or a selective β 2 -antagonist (ICI 118,551) was injected 15·min prior to the isoprenaline infusion. Both noradrenaline and isoprenaline led to an expected rise in glucose concentration. Isoprenaline combined with both the β 1 -and β 2 -antagonist led to higher glucose concentrations than isoprenaline alone. This could indicate the presence of a stimulatory β-adrenoceptor different from β 1 and β 2 -adrenoceptors; these two receptors thus seemed to mediate a reduction in plasma glucose concentration. Both noradrenaline and isoprenaline led to a significant decrease in FFA concentration. Whereas the β 1 -antagonist had no effect, the β 2 -antagonist reduced the decrease in FFA concentration, indicating the involvement of β 2 -adrenoceptors. It is concluded that the air-breathing African catfish reflects water-breathing fish in the adrenergic control of plasma FFA and glucose levels.

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“…With the exception of Xenopus and turkey [14–16], no studies have dealt with the molecular biology of nonmammalian β‐ARs. Physiology‐based studies using several species of fishes reveal that some of the typical mammalian β‐adrenergic responses including increased cardiac output and elevated blood glucose levels also occur in fish through agonist mediated β‐AR signaling [7,17]. In addition, fish β‐ARs elicit responses unique to particular species or groups of fish.…”

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confidence: 99%

A putative β₂‐adrenoceptor from the rainbow trout (Oncorhynuchus mykiss)

Nickerson

Dugan

Drouin

et al. 2001

European Journal of Biochemistry

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Extensive molecular characterization of mammalian b-adrenoceptors has revealed complex modes of regulation and interaction. Relatively little attention, however, has focused on adrenoceptors from early branching vertebrates such as fish. Using an RT-PCR approach we have cloned a rainbow trout b 2 -adrenoceptor gene that codes for a 409-amino-acid protein with the same seven transmembrane domain structure as its mammalian counterparts. This rainbow trout b 2 -adrenoceptor shares a high degree of amino-acid sequence conservation with other vertebrate b 2 -adrenoceptors. The conclusion that this sequence is a rainbow trout b 2 -adrenoceptor is further supported by phylogenetic analysis of vertebrate b-adrenoceptor sequences and competitive pharmacological binding data. RNase protection assays demonstrate that the rainbow trout b 2 -adrenoceptor gene is highly expressed in the liver and red and white muscle, with lower levels of expression in the gills, heart, kidney and spleen of the rainbow trout. The lack of regulatory phosphorylation sites within the G-proteinbinding domain of the rainbow trout b 2 -adrenoceptor sequence suggests that the in vivo control of trout b 2 -adrenoceptor signaling differs substantially from that of mammals.

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Characterization of [3H]CGP 12177 Binding to β-Adrenergic Receptors in Intact Eel Hepatocytes

Cited by 10 publications

References 17 publications

Regional distribution and cellular localization of β₂‐adrenoceptors in the adult zebrafish brain (Danio rerio)

Regional distribution and cellular localization of β₂‐adrenoceptors in the adult zebrafish brain (Danio rerio)

Beta-adrenergic control of plasma glucose and free fatty acid levels in the air-breathing African catfishClarias gariepinusBurchell 1822

A putative β₂‐adrenoceptor from the rainbow trout (Oncorhynuchus mykiss)

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Characterization of [3H]CGP 12177 Binding to β-Adrenergic Receptors in Intact Eel Hepatocytes

Cited by 10 publications

References 17 publications

Regional distribution and cellular localization of β2‐adrenoceptors in the adult zebrafish brain (Danio rerio)

Regional distribution and cellular localization of β2‐adrenoceptors in the adult zebrafish brain (Danio rerio)

Beta-adrenergic control of plasma glucose and free fatty acid levels in the air-breathing African catfishClarias gariepinusBurchell 1822

A putative β2‐adrenoceptor from the rainbow trout (Oncorhynuchus mykiss)

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Product

Resources

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Regional distribution and cellular localization of β₂‐adrenoceptors in the adult zebrafish brain (Danio rerio)

Regional distribution and cellular localization of β₂‐adrenoceptors in the adult zebrafish brain (Danio rerio)

A putative β₂‐adrenoceptor from the rainbow trout (Oncorhynuchus mykiss)