Characterization of 3-D collagen hydrogels for functional cell-based biosensing

Chen, Mao; Kisaalita, William S.

doi:10.1016/j.bios.2003.10.008

Cited by 50 publications

(53 citation statements)

References 33 publications

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“…Unfortunately, IMR-32 cells do not develop a resting membrane potential (V m ) characteristic of nerve cells (Rao and Kisaalita, 2001). Also, Mao and Kisaalita (2004a) comparative proliferation results were not conclusive. The purpose of this paper is to extend the study to a human neuroblastoma cell line that develops a neuronallike resting membrane potential, and to compare cellular proliferation between the traditional 2-D and 3-D collagen hydrogel.…”

Section: Introductionmentioning

confidence: 78%

“…In a previous paper, Mao and Kisaalita (2004a) studied voltage gated calcium channel (VGCC) properties of the neuroblastoma cell line, IMR-32 in a 3-D collagen hydrogel, and concluded that the optical and mechanical properties of collagen hydrogel (0.5-1.0 mg/ml collagen) are suitable for a cell-based biosensor. Furthermore, Mao and Kisaalita (2004a,b) showed a significant difference in calcium response to high K + depolarization between cells grown in 2-D flat dishes (monolayer) versus 3-D hydrogel.…”

Section: Introductionmentioning

confidence: 98%

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Human neuroblastoma (SH-SY5Y) cell culture and differentiation in 3-D collagen hydrogels for cell-based biosensing

Desai

Kisaalita

Keith

et al. 2006

Biosensors and Bioelectronics

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Section: Introductionmentioning

confidence: 78%

Section: Introductionmentioning

confidence: 98%

Human neuroblastoma (SH-SY5Y) cell culture and differentiation in 3-D collagen hydrogels for cell-based biosensing

Desai

Kisaalita

Keith

et al. 2006

Biosensors and Bioelectronics

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“…Valero and coworkers [309], have studied the impact of different soft matrices on neuronal differentiation of the Neuro2a neuroblastoma cell line (N2a), evaluated with a novel impedimetric biosensor. The authors showed that neither agarose nor bare collagen was able to support the differentiation of N2a cells.…”

Section: Nbl Cells On Scaffoldsmentioning

confidence: 99%

In Vitro Modeling of Tissue-Specific 3D Microenvironments and Possibile Application to Pediatric Cancer Research

Pizzimenti¹

2014

J. Pediatr. Oncol.

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A large body of evidence indicates that three dimensional (3D) cancer models are superior to two-dimensional (2D) ones in better representing the in vivo phenomena. Indeed, 3D models allow recapitulating in vitro the in vivo features observed in solid tumors (e.g. cell polarity, cell-cell/cell-matrix interactions, biochemical/metabolic gradients, anchorage-independent growth and hypoxia). Moreover, it is well established that the microenvironment plays a fundamental role in regulating tumor development and behavior, including drug resistance. Thus, innovative models able to mimic this complexity represent attractive tools in cancer research. In this review article, we provide a comprehensive review of the application of 3D culture systems in pediatrics' cancer research. In particular, 3D in vitro/ex vivo models of the most common pediatric tumors, such as leukemias, lymphomas and malignancies of the nervous system, will be considered.

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“…31,32 Application of mammalian cells in collagen-entrapped form for use as biosensors is still in its infancy, 34,35 but offers significant promise. [34][35][36] Previous studies from our laboratory have shown that a mouse B-cell hybridoma (Ped-2E9)-based cytotoxicity assay can be used to detect the presence of pathogenic Listeria species and the enterotoxins from Bacillus species. 16,37,38 This cytotoxicity assay can also differentiate the presence of viable pathogenic Listeria species from nonpathogenic ones.…”

mentioning

confidence: 99%

A novel and simple cell-based detection system with a collagen-encapsulated B-lymphocyte cell line as a biosensor for rapid detection of pathogens and toxins

Banerjee

Lenz

Robinson

et al. 2008

Laboratory Investigation

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Cell-based biosensors (CBBs) are becoming important tools for biosecurity applications and rapid diagnostics in food microbiology for their unique capability of detecting physiologically hazardous materials. A multi-well plate-based biosensor containing B-cell hybridoma, Ped-2E9, encapsulated in type I collagen matrix, was developed for rapid detection of viable cells of pathogenic Listeria, the toxin listeriolysin O, and the enterotoxin from Bacillus species. This sensor measures the alkaline phosphatase release from infected Ped-2E9 cells colorimetrically. Pathogenic L. monocytogenes cells and toxin preparations from L. monocytogenes or B. cereus showed cytotoxicity ranging from 24 to 98% at 3-6 h postinfection. In contrast, nonpathogenic L. innocua (F4247) and B. subtilis induced minimal cytotoxicity, ranging only 0.4-7.6%. Laser scanning cytometry and cryo-nano scanning electron microscopy confirmed the live or dead status of the infected Ped-2E9 cells in gel matrix. This paper presents the first example of a cell-based sensing system using collagen-encapsulated mammalian cells for rapid detection of pathogenic bacteria or toxin, and demonstrates a potential for onsite use as a portable detection system.

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Characterization of 3-D collagen hydrogels for functional cell-based biosensing

Cited by 50 publications

References 33 publications

Human neuroblastoma (SH-SY5Y) cell culture and differentiation in 3-D collagen hydrogels for cell-based biosensing

Human neuroblastoma (SH-SY5Y) cell culture and differentiation in 3-D collagen hydrogels for cell-based biosensing

In Vitro Modeling of Tissue-Specific 3D Microenvironments and Possibile Application to Pediatric Cancer Research

A novel and simple cell-based detection system with a collagen-encapsulated B-lymphocyte cell line as a biosensor for rapid detection of pathogens and toxins

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