Characterization of 25-Hydroxyvitamin D Binding Protein from Intestinal Cells

Teegarden, Dorothy; Nickel, K.P.; Shi, Lingling

doi:10.1006/bbrc.2000.3397

Cited by 15 publications

(8 citation statements)

References 24 publications

(18 reference statements)

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“…On the contrary, high expression of IDBP may be a compensatory mechanism to enhance vitamin D action in the face of a functional vitamin D de ciency. The notion of a reciprocal relation between 1,25-dihydroxyvitamin D action and cellular IDBP concentration would be consistent with the observations of Teegarden et al 11 on the role of 1,25-dihydroxyvitamin D on 25-hydroxyvitamin D-binding capacity. These investigators found that the level of a 68-kDa cytosolic vitamin D-binding protein was decreased by 1,25-dihydroxyvitamin D treatment in Caco-2 cells, a human intestinal cell line.…”

supporting

confidence: 90%

Novel Intracellular Proteins Associated with Cellular Vitamin D Action

Angelo

Wood²,

Mayer³

2002

Nutrition Reviews

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Work with vitamin D–resistant New World primates has revealed novel cellular proteins involved in vitamin D action. An “intracellular vitamin D–binding protein” functions to bind vitamin D metabolites in the cell and enhances vitamin D action. By contrast, a “vitamin D response element–binding protein” inhibits vitamin D receptor binding to the DNA and is responsible for vitamin D resistance in New World primates.

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supporting

confidence: 90%

Novel Intracellular Proteins Associated with Cellular Vitamin D Action

Angelo

Wood²,

Mayer³

2002

Nutrition Reviews

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“…Besides, the efflux of D 3 from the cell compartment to the apical medium, which represents the intestinal lumen, was significantly more important than that of 25(OH)D 3 and 1α(OH)D 3 . This result may be explained by the presence of a cytosolic vitamin D binding protein (cDBP) [ 25 ], which would display a higher affinity for hydroxylated forms than for D 3 , similarly to plasma DBP [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

Comparison of the Micellar Incorporation and the Intestinal Cell Uptake of Cholecalciferol, 25-Hydroxycholecalciferol and 1-α-Hydroxycholecalciferol

Desmarchelier

Margier

Prévéraud³

et al. 2017

Nutrients

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In the context of the global prevalence of vitamin D insufficiency, we compared two key determinants of the bioavailability of 3 vitamin D forms with significant biopotencies: cholecalciferol, 25-hydroxycholecalciferol and 1-α-hydroxycholecalciferol. To this aim, we studied their incorporation into synthetic mixed micelles and their uptake by intestinal cells in culture. Our results show that 1-α-hydroxycholecalciferol was significantly more solubilized into mixed micelles compared to the other forms (1.6-fold and 2.9-fold improvement compared to cholecalciferol and 25-hydroxycholecalciferol, respectively). In Caco-2 TC7 cells, the hydroxylated forms were taken up more efficiently than cholecalciferol (p < 0.05), and conversely to cholecalciferol, their uptake was neither SR-BI(Scavenger-Receptor class B type I)- nor NPC1L1 (NPC1 like intracellular cholesterol transporter 1)-dependent. Besides, the apical membrane sodium–bile acid transporter ASBT (Apical Sodium-dependent Bile acid Transporter) was not involved, at least in vitro, in the uptake of any of the three vitamin D forms. Further investigations are needed to identify the uptake pathways of both 1-α-hydroxycholecalciferol and 25-hydroxycholecalciferol. However, considering its high bioavailability, our results suggest the potential interest of using 1-α-hydroxycholecalciferol in the treatment of severe vitamin D deficiency.

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“…25OHD is transported in the blood by a well-characterized vitamin D-binding protein, which has a higher affinity for 25OHD than for 1,25(OH) 2 D and native vitamin D, and which is present in large excess of the normal blood contents of its ligands [54]. A specific vitamin D-binding protein has been demonstrated in the cytosol from rat and human intestinal cells, which binds 25OHD with at least 1,000-fold greater affinity than 1,25(OH) 2 D, and thus may be involved in the absorption of 25OHD, or the calcium absorptive actions of 25OHD [55,56]. Yet another intracellular vitamin D-binding protein, which preferently binds 25OHD (3-fold greater affinity than for 1,25(OH) 2 D) has been characterized in New World monkeys [57].…”

Section: Bioavailability Homeostasis and Transportmentioning

confidence: 99%

Food Contents and Biological Activity of 25-Hydroxyvitamin D: A Vitamin D Metabolite to Be Reckoned With?

Ovesen

Brot

Jakobsen³

2003

Ann Nutr Metab

176

114

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Only a limited number of foods naturally contain vitamin D such as fish, meat and offal, and eggs, and milk and dairy products. However, all these foods in addition contain the metabolite 25-hydroxyvitamin D (25OHD). From the few systematic studies which have been performed the food contents of 25OHD in animal foods are usually low but vary. Contents are typically very low in milk and fish (<0.1 µg/100 g), somewhat higher in meat and offal (0.2–0.4 µg/100 g) and up to 1 µg/100 g in egg yolk. It has been demonstrated that 25OHD is absorbed better and faster from the diet than native vitamin D and has metabolic effects of its own in regulating cell growth and calcium metabolism. Thus, the biological activity of 25OHD is greater than that of native vitamin D. However, there is as yet no consensus on the conversion factor that should be used for 25OHD to calculate vitamin D activity. Depending on the testing system used the factor varies from 1.5 to 5. If food contents and the higher potency of 25OHD are not included in dietary intake surveys, true vitamin D intake will be underestimated.

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Characterization of 25-Hydroxyvitamin D Binding Protein from Intestinal Cells

Cited by 15 publications

References 24 publications

Novel Intracellular Proteins Associated with Cellular Vitamin D Action

Novel Intracellular Proteins Associated with Cellular Vitamin D Action

Comparison of the Micellar Incorporation and the Intestinal Cell Uptake of Cholecalciferol, 25-Hydroxycholecalciferol and 1-α-Hydroxycholecalciferol

Food Contents and Biological Activity of 25-Hydroxyvitamin D: A Vitamin D Metabolite to Be Reckoned With?

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