Characterization of 19 novel cotton <i>FLA</i> genes and their expression profiling in fiber development and in response to phytohormones and salt stress

Huang, Geng-Qing; Xu, Wen‐Liang; Gong, Si-Ying; Li, Bing; Wang, Xiulan; Xu, Dan; Li, Xuebao

doi:10.1111/j.1399-3054.2008.01139.x

Cited by 74 publications

(77 citation statements)

References 45 publications

(82 reference statements)

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“…A study reported that GhAGP4 plays a role in fiber initiation and elongation of cotton, but its molecular mechanism in regulating fiber development still remains unclear (Li et al, 2010b). In our previous study, several fiber-preferential genes encoding FLAs were identified in cotton (Huang et al, 2008). In this study, we further demonstrate that cotton FLA1 is involved in fiber initiation and elongation.…”

supporting

confidence: 50%

“…Our previous study demonstrated the several GhFLAs were expressed at high levels in 5-to 20-DPA fibers (Huang et al, 2008). In addition, bioinformatic analysis indicated the presence of at least five classical AGP sequences in cotton fibers.…”

Section: Discussionmentioning

confidence: 99%

“…Through screening the fiber complementary DNA (cDNA) library and the public cotton EST database, seven FLA genes that are specifically or predominantly expressed in fibers were identified in cotton (Huang et al, 2008). To elucidate the role of the FLA proteins in fiber development, we selected GhFLA1 (GenBank accession no.…”

Section: Isolation and Characterization Of Cotton Ghfla1mentioning

confidence: 99%

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A Fasciclin-Like Arabinogalactan Protein, GhFLA1, Is Involved in Fiber Initiation and Elongation of Cotton

et al. 2013

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Arabinogalactan proteins (AGPs) are involved in many aspects of plant development. In this study, biochemical and genetic approaches demonstrated that AGPs are abundant in developing fibers and may be involved in fiber initiation and elongation. To further investigate the role of AGPs during fiber development, a fasciclin-like arabinogalactan protein gene (GhFLA1) was identified in cotton (Gossypium hirsutum). Overexpression of GhFLA1 in cotton promoted fiber elongation, leading to an increase in fiber length. In contrast, suppression of GhFLA1 expression in cotton slowed down fiber initiation and elongation. As a result, the mature fibers of the transgenic plants were significantly shorter than those of the wild type. In addition, expression levels of GhFLAs and the genes related to primary cell wall biosynthesis were remarkably enhanced in the GhFLA1 overexpression transgenic fibers, whereas the transcripts of these genes were dramatically reduced in the fibers of GhFLA1 RNA interference plants. An immunostaining assay indicated that both AGP composition and primary cell wall composition were changed in the transgenic fibers. The levels of glucose, arabinose, and galactose were also altered in the primary cell wall of the transgenic fibers compared with those of the wild type. Together, our results suggested that GhFLA1 may function in fiber initiation and elongation by affecting AGP composition and the integrity of the primary cell wall matrix.

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confidence: 50%

Section: Discussionmentioning

confidence: 99%

Section: Isolation and Characterization Of Cotton Ghfla1mentioning

confidence: 99%

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A Fasciclin-Like Arabinogalactan Protein, GhFLA1, Is Involved in Fiber Initiation and Elongation of Cotton

et al. 2013

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“…Similarly, previous studies have revealed that the transcripts of GhFLA1, GhFLA2, and GhFLA4 are accumulated at relatively high levels in fibers. As a result, the highest levels of GhFLA1 and GhFLA4 transcripts are detected in 10 DPA fibers, while GhFLA2 shows its highest expression level in 20 DPA fibers [33]. GaMYB2 transcripts are largely accumulated in 3-9 DPA fibers, and then decline with further fiber development [6].…”

Section: Discussionmentioning

confidence: 91%

A cotton gene encoding novel MADS-box protein is preferentially expressed in fibers and functions in cell elongation

Li¹,

Hua²,

Zhang³

et al. 2011

ABBS

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“…These authors identified 5 different AGPs that were strongly repressed in salt treated tomato roots. Similarly, Huang et al analyzed the differential expression of 19 putative fasciclin-like proteins in Gossypium hirsutum in plants treated with different phytohormones and NaCl (Huang et al, 2008). These authors observed that several fasciclin-like proteins were down regulated by NaCl and ABA.…”

Section: Discussionmentioning

confidence: 99%

Arabinogalactan Proteins Are Involved in Salt-Adaptation and Vesicle Trafficking in Tobacco by-2 Cell Cultures

et al. 2017

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Arabinogalactan proteins (AGPs) are a highly diverse family of glycoproteins that are commonly found in most plant species. However, little is known about the physiological and molecular mechanisms of their function. AGPs are involved in different biological processes such as cell differentiation, cell expansion, tissue development and somatic embryogenesis. AGPs are also involved in abiotic stress response such as salinity modulating cell wall expansion. In this study, we describe how salt-adaptation in tobacco BY-2 cell cultures induces important changes in arabinogalactan proteins distribution and contents. Using the immuno-dot blot technique with different anti-AGP antibodies (JIM13, JIM15, and others), we observed that AGPs were highly accumulated in the culture medium of salt-adapted tobacco cells, probably due to the action of phospholipases. We located these AGP epitopes using immunogold labeling in the cytoplasm associated to the endoplasmic reticulum, the golgi apparatus, and vesicles, plasma membrane and tonoplast. Our results show that salt-adaptation induced a significant reduction of the cytoplasm, plasma membrane and tonoplast content of these epitopes. Yariv reagent was added to the control and salt-adapted tobacco cell cultures, leading to cell death induction in control cells but not in salt-adapted cells. Ultrastructural and immunogold labeling revealed that cell death induced by Yariv reagent in control cells was due to the interaction of Yariv reagent with the AGPs linked to the plasma membranes. Finally, we propose a new function of AGPs as a possible sodium carrier through the mechanism of vesicle trafficking from the apoplast to the vacuoles in salt-adapted tobacco BY-2 cells. This mechanism may contribute to sodium homeostasis during salt-adaptation to high saline concentrations.

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Characterization of 19 novel cotton FLA genes and their expression profiling in fiber development and in response to phytohormones and salt stress

Cited by 74 publications

References 45 publications

A Fasciclin-Like Arabinogalactan Protein, GhFLA1, Is Involved in Fiber Initiation and Elongation of Cotton

A Fasciclin-Like Arabinogalactan Protein, GhFLA1, Is Involved in Fiber Initiation and Elongation of Cotton

A cotton gene encoding novel MADS-box protein is preferentially expressed in fibers and functions in cell elongation

Arabinogalactan Proteins Are Involved in Salt-Adaptation and Vesicle Trafficking in Tobacco by-2 Cell Cultures

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