Characterization of 13 microsatellite markers for <i>Calochortus gunnisonii</i> (Liliaceae) from Illumina MiSeq sequencing

Fuller, Ryan S.; Frietze, Seth; McGlaughlin, Mitchell E.

doi:10.3732/apps.1500051

Cited by 1 publication

(1 citation statement)

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“…All individuals were analyzed for 12 microsatellite loci as described in Fuller et al. (). Amplification products were diluted with water and mixed with Hi‐Di formamide and LIZ 500 size standard (Applied Biosystems, Foster City, California, USA) before electrophoresis on an Applied Biosystems 3730 Genetic Analyzer at Arizona State University Core DNA Laboratory (Tempe, Arizona, USA).…”

Section: Methodsmentioning

confidence: 99%

Calochortus gunnisonii furthers evidence for the complex genetic legacy of historical climate change in the southern Rocky Mountains

Fuller

McGlaughlin

2019

American J of Botany

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Premise of the Study Climate cycles of the Quaternary have impacted plants at a global scale, leaving behind a complex genetic legacy. Species of the northern Rocky Mountains of North America were exposed to more uniform glacial patterns than the central and southern ranges, where synergistic relationships between temperature and precipitation caused differences in the timing and extent of glacier onset. We examined the genetic impacts of climate oscillations on Calochortus gunnisonii (Liliaceae) in the central and southern Rocky Mountains. Methods Populations were sampled from disjunct mountain ranges across the basins of Wyoming and northern and central Colorado. Allelic data from nuclear microsatellites and plastid sequences (trnV‐ndhC, petA‐psbJ, and rpl16) were used to examine patterns of genetic structure between and among populations along the southern Rocky Mountain corridor. Key Results We infer considerable population structure concordant with mountain range of origin. Clustering analysis supports separate north and south genetic clusters on either side of major basins in Wyoming, suggesting that populations were maintained in two distinct refugia. Additionally, populations within the Sierra Madre Range of southern Wyoming show localized, divergent genetic signal indicative of a third potential glacial refugium. By contrast, recent genetic admixture is observed in the Laramie, Medicine Bow, and Front ranges, where population expansion from glacial refugia has likely occurred. Conclusions We conclude that during climate cycles of the Quaternary, C. gunnisonii experienced periods of population expansion and reduction, habitat fragmentation, isolation in three or more refugia, and admixture mirroring genetic impacts of other southern Rocky Mountains organisms.

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Section: Methodsmentioning

confidence: 99%