Characterization of 1-Aminocyclopropane-1-Carboxylate (ACC) Deaminase-Containing Pseudomonas spp. in the Rhizosphere of Salt-Stressed Canola

Akhgar, Abdolreza; Arzanlou, Mahdi; Bakker, Peter A. H. M.; Hamidpour, Mohsen

doi:10.1016/s1002-0160(14)60032-1

Cited by 54 publications

(15 citation statements)

References 41 publications

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“…These results should be interpreted cautiously, however, as 16S rDNA sequence analysis was conducted on bacteria selectively enriched in the presence of ACC under laboratory conditions. Using this type of approach, others have also reported Pseudomonas to be a dominant genus of ACC+ bacteria in plant rhizospheres (e.g., Timmusk et al, 2011;Akhgar et al, 2014). Future studies should focus on developing and applying molecular-based methods to characterize the genetic diversity of nonculturable ACC+ bacteria in soil.…”

Section: Discussionmentioning

confidence: 99%

Genotype-Specific Enrichment of 1-Aminocyclopropane-1-Carboxylic Acid Deaminase-Positive Bacteria in Winter Wheat Rhizospheres

Stromberger

Abduelafez

Byrne

et al. 2017

Soil Science Society of America Journal

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Bacteria that produce 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase can promote plant growth under abiotic stress by lowering stress ethylene levels through deamination of ACC, the immediate precursor of ethylene. Unfortunately, little is known regarding the natural abundance and diversity of ACC deaminase-positive (ACC+) bacteria in soils or how ACC+ bacteria are influenced by plant genotype. Two field studies were conducted to assess the abundance, composition, and ACC deaminase activity of ACC+ bacteria in plots planted to different winter wheat (Triticum aestivum L.) genotypes under different irrigation regimes. In the first study, the relative abundance of ACC+ bacteria in wheat rhizospheres increased over time as soil water availability decreased. Relative abundance was also affected by genotype, with the greatest percentage of ACC+ bacteria in the rhizosphere of 'RonL' grown with limited or no irrigation (up to 54% at mid-grain filling). Species composition also varied by wheat genotype regardless of irrigation treatment. In the second study, the RonL rhizosphere had the greatest ACC deaminase activity and greatest predicted abundance of ACC+ bacteria, on the basis of Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) analysis of 16S rDNA sequences, compared with other genotypes. In conclusion, the relative abundance, composition, and activity of culturable and predicted communities of ACC+ bacteria differed according to winter wheat genotype; therefore, the potential for ACC+ bacteria to promote drought resistance in winter wheat may be genotype-dependent. A mong the many ecosystem services provided by soil bacteria, perhaps none is as important within agricultural systems as supporting plant growth. Soil bacteria that influence plant growth positively are called plant growth-promoting bacteria (Bashan and Holguin, 1998). These bacteria reside within root tissues, on the root surface, and in the rhizosphere soil, where they can improve plant growth by increasing the availability of nutrients to plants, producing phytohormones that regulate plant growth, and antagonizing and outcompeting plant pathogens in the soil environment (Lugtenberg and Kamilova, 2009;Chaparro et al., 2012).Water scarcity is among the most difficult of the challenges facing agricultural sustainability and food security. Of growing interest is the ability of certain PGPRs to reduce the effects on plants of abiotic stress in the environment, includ- Core Ideas• 1-Aminocyclopropane-1-carboxylic acid (ACC) deaminase-positive (ACC+) bacteria were studied in field soils planted to winter wheat.• The relative abundance of ACC+ bacteria increased in wheat rhizospheres as soil water availability decreased.• ACC+ bacterial abundance, composition, and deaminase activity were influenced by wheat genotype.• The ability of ACC+ bacteria to promote drought resistance may be dependent on wheat genotype.

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Section: Discussionmentioning

confidence: 99%

Genotype-Specific Enrichment of 1-Aminocyclopropane-1-Carboxylic Acid Deaminase-Positive Bacteria in Winter Wheat Rhizospheres

Stromberger

Abduelafez

Byrne

et al. 2017

Soil Science Society of America Journal

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“…Twenty seven percent of the isolated bacterial strains were found to possess 1‐aminocyclopropane‐1‐carboxylate (ACC) deaminase activity. The hormone ethylene plays a role in various physiological processes in plants, including the breaking of seed dormancy, but sustained high levels in response to biotic and abiotic stress can inhibit root growth and induce senescence (Akhgar et al., ). Endophytic bacteria that can produce the enzyme ACC deaminase contribute towards the catabolism of the plant ethylene precursor, ACC, consequently decreasing plant ethylene level and enabling plants to better tolerate biotic and abiotic stresses (Glick et al., , Glick et al., ).…”

Section: Discussionmentioning

confidence: 99%

“…Endophytic bacteria that can produce the enzyme ACC deaminase contribute towards the catabolism of the plant ethylene precursor, ACC, consequently decreasing plant ethylene level and enabling plants to better tolerate biotic and abiotic stresses (Glick et al., , Glick et al., ). Microbial ability to produce ACC deaminase has been identified as one of the direct mechanisms of plant growth‐promotion and has been linked to drought and salt tolerance in various plant species (Akhgar et al., ; Glick, ; Sgroy et al., ).…”

Section: Discussionmentioning

confidence: 99%

Brachiaria Grasses (Brachiaria spp.) harbor a diverse bacterial community with multiple attributes beneficial to plant growth and development

2017

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Endophytic and plant‐associated bacteria were isolated from plants and rhizoplane soil of naturally grown Brachiaria grasses at International Livestock Research Institute in Nairobi, Kenya. Eighty‐four bacterial strains were isolated from leaf tissues, root tissues, and rhizoplane soil on nutrient agar and 869 media. All bacterial strains were identified to the lowest possible taxonomic unit using 16S rDNA primers and were characterized for the production of Indole‐3‐acetic acid, hydrogen cyanide, and ACC deaminase; phosphate solubilization; siderophore production; antifungal properties; and plant biomass production. The 16S rDNA‐based identification grouped these 84 bacterial strains into 3 phyla, 5 classes, 8 orders, 12 families, 16 genera, and 50 unique taxa. The four most frequently isolated genera were Pseudomonas (23), Pantoea (17), Acinetobacter (9), and Enterobacter (8). The functional characterization of these strains revealed that 41 of 84 strains had a minimum of three plant beneficial properties. Inoculation of maize seedlings with Acinetobacter spp., Microbacterium spp., Pectobacterium spp., Pseudomonas spp., and Enterobacter spp. showed positive effects on seedling biomass production. The ability of Brachiaria grasses to host genetically diverse bacteria, many of them with multiple plant growth‐promoting attributes, might have contributed to high biomass production and adaptation of Brachiaria grasses to drought and low fertility soils.

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“…In P. palleroniana Ps006, more genes involved in potassium, nitrogen, iron, sulfur, and phosphorus metabolism were found, whereas in B. amyloliquefaciens BS006, only sporulation factors were notably more abundant (Tables 1 and 2). As for plant stress and defense, PGPR can aid in active plant growth in conditions of abiotic or biotic stresses [53]. PGPR produce repressive substances that increase the natural resistance to phytopathogens and pests [9], such as hydrolytic enzymes (chitinases, cellulases, and proteases), as well as various antibiotics, volatile organic compounds (VOCs), exopolysaccharides (EPS), and siderophores that protect plants against phytopathogens [9,54].…”

Section: Differences In the Genetic Background Explain Different Colomentioning

confidence: 99%

Complementary Dynamics of Banana Root Colonization by the Plant Growth-Promoting Rhizobacteria Bacillus amyloliquefaciens Bs006 and Pseudomonas palleroniana Ps006 at Spatial and Temporal Scales

et al. 2020

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Banana (Musa acuminata) growth for commercial purposes requires high amounts of chemical fertilizers, generating high costs and deleterious effects on the environment. In a previous study, we demonstrated that two plant growthpromoting rhizobacteria (PGPR), Bacillus amyloliquefaciens Bs006 and Pseudomonas palleroniana Ps006, isolated in Colombia, could partially replace chemical fertilizers for banana seedling growth. In a second work, the effects of the two inoculants on banana transcripts were found to occur at different times, earlier for Bs006 and later for Ps006. This leads to the hypothesis that the two rhizobacteria have different colonization dynamics. Accordingly, the aim of this work was to analyze the dynamics of root colonization of the two PGPR, Bs006 and Ps006, on banana growth over a time frame of 30 days. We used fluorescence in situ hybridization (FISH) and confocal laser scanning microscopy (CLSM), followed by three-dimensional reconstruction and quantitative image analysis. Bacillus amyloliquefaciens Bs006 abundantly colonized banana roots earlier (from 1 to 48 h), ectophytically on the rhizoplane, and then decreased. Pseudomonas palleroniana Ps006 was initially scarce, but after 96 h it increased dramatically and became clearly endophytic. Here we identify and discuss the potential genetic factors responsible for this complementary behavior. This information is crucial for optimizing the formulation of an effective biofertilizer for banana and its inoculation strategy.

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Characterization of 1-Aminocyclopropane-1-Carboxylate (ACC) Deaminase-Containing Pseudomonas spp. in the Rhizosphere of Salt-Stressed Canola

Cited by 54 publications

References 41 publications

Genotype-Specific Enrichment of 1-Aminocyclopropane-1-Carboxylic Acid Deaminase-Positive Bacteria in Winter Wheat Rhizospheres

Genotype-Specific Enrichment of 1-Aminocyclopropane-1-Carboxylic Acid Deaminase-Positive Bacteria in Winter Wheat Rhizospheres

Brachiaria Grasses (Brachiaria spp.) harbor a diverse bacterial community with multiple attributes beneficial to plant growth and development

Complementary Dynamics of Banana Root Colonization by the Plant Growth-Promoting Rhizobacteria Bacillus amyloliquefaciens Bs006 and Pseudomonas palleroniana Ps006 at Spatial and Temporal Scales

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