Characterization, modes of action, and application of a novel broad-spectrum bacteriocin BM1300 produced by Lactobacillus crustorum MN047

Lü, Xin; Rakhmanova, Aizhan; Yan, Hong; Li, Xin; Wang, Xin; Yi, Yanglei; Shan, Yuanyuan; Liu, Bianfang; Zhou, Yuan; Lü, Xin

doi:10.1007/s42770-020-00311-3

Cited by 27 publications

(16 citation statements)

References 44 publications

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“…Bacterial lactate dehydrogenase (LDH), as one of the membranebound enzymes, belongs to the oxidoreductase isoenzyme in the respiratory pathway contributing to proper functioning of the bacterial metabolism. 51 The measurement of LDH activity could account for the effect of the bacterial metabolism on compound 5j. As is demonstrated in Figure 7, the LDH activity appeared a downtrend accomplished with an augmented amount of compound 5j, hinting that respiratory functions of E. coli and P. aeruginosa were meaningfully influenced by compound 5j.…”

Section: ■ Introductionmentioning

confidence: 99%

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Identification of Unique Quinazolone Thiazoles as Novel Structural Scaffolds for Potential Gram-Negative Bacterial Conquerors

2021

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A class of quinazolone thiazoles was identified as new structural scaffolds for potential antibacterial conquerors to tackle dreadful resistance. Some prepared compounds exhibited favorable bacteriostatic efficiencies on tested bacteria, and the most representative 5j featuring the 4-trifluoromethylphenyl group possessed superior performances against Escherichia coli and Pseudomonas aeruginosa to norfloxacin. Further studies revealed that 5j with inappreciable hemolysis could hinder the formation of bacterial biofilms and trigger reactive oxygen species generation, which could take responsibility for emerging low resistance. Subsequent paralleled exploration discovered that 5j not only disintegrated outer and inner membranes to induce leakage of cytoplasmic contents but also broke the metabolism by suppressing dehydrogenase. Meanwhile, derivative 5j could intercalate into DNA to exert powerful antibacterial properties. Moreover, compound 5j gave synergistic effects against some Gram-negative bacteria in combination with norfloxacin. These findings indicated that this novel structural type of quinazolone thiazoles showed therapeutic foreground in struggling with Gram-negative bacterial infections.

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Section: ■ Introductionmentioning

confidence: 99%

“…Bacterial lactate dehydrogenase (LDH), as one of the membrane-bound enzymes, belongs to the oxidoreductase isoenzyme in the respiratory pathway contributing to proper functioning of the bacterial metabolism . The measurement of LDH activity could account for the effect of the bacterial metabolism on compound 5j .…”

Section: Introductionmentioning

confidence: 99%

Identification of Unique Quinazolone Thiazoles as Novel Structural Scaffolds for Potential Gram-Negative Bacterial Conquerors

2021

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“…In the present study, LSB1 was confirmed to possess activity against biofilm formation by S. argenteus_70917, as determined by live‐death bacteria fluorescence detection and quantification of biofilms by crystal violet staining. Previous studies have reported the antibiofilm activity of LAB bacteriocins, such as bacteriocin CFS24 against L. innocua biofilm (46.7% inhibitory rate), BM1300 against E. coli biofilm (69.2% inhibitory rate), and BM173 against S. aureus biofilm (55.1% inhibitory rate) (Kátia et al., 2019; Lu et al., 2020; Qiao et al., 2020). The present study thus demonstrated a good activity of LSB1 against biofilm formation of S. argenteus _70917.…”

Section: Resultsmentioning

confidence: 99%

Purification and characterization of Lactobacillus plantarum‐derived bacteriocin with activity against Staphylococcus argenteus planktonic cells and biofilm

Zhang

Jiang

et al. 2022

Journal of Food Science

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Bacteriocins inhibit various foodborne bacteria in planktonic and biofilm forms. However, bacteriocins with antibacterial and antibiofilm activity against Staphylococcus argenteus, a pathogen that can cause food poisoning, are still poorly known. Here, the novel bacteriocin LSB1 derived from Lactobacillus plantarum CGMCC 1.12934 was purified and characterized extensively. LSB1 had a molecular weight of 1425.78 Da and an amino acid sequence of YIFVTGGVVSSLGK. Moreover, LSB1 exhibited excellent stability under heat and acid–base stress and presented sensitivity to pepsin and proteinase K. LSB1 exhibited an extensive antimicrobial spectrum against both Gram‐positive and Gram‐negative bacteria. Minimum inhibitory concentration of LSB1 against S. argenteus_70917 was 10.36 µg/ml, which was lower than that of most of the previously found bacteriocins against Staphylococcus strains. Furthermore, LSB1 significantly inhibited S. argenteus_70917 planktonic cells (p < 0.01) and decreased their viability. Scanning electron microscopy analysis revealed that cell membrane permeability of S. argenteus_70917 upon exposure to LSB1 showed leakage of cytoplasmic contents and rupture, leading to cell death. In addition, biofilm formation ability of S. argenteus_70917 was significantly (p < 0.01) impaired by LSB1, with the percent inhibition of 35% at 10 µg/ml and 80% at 20 µg/ml. Overall, this study indicates that LSB1 can be considered a potential antibacterial agent in the control of S. argenteus in both planktonic and biofilm states. Practical Application Foodborne pathogenic bacteria, such as Staphylococcus argenteus, and their biofilms represent potential risks for food safety. In recent years, customers’ demand for “natural” products has increased food control. This study describes the novel bacteriocin LSB1 produced by the lactic acid bacterium species Lactobacillus plantarum. LSB1 showed strong antibacterial and antibiofilm activity against S. argenteus as well as thermal and acid–alkaline stability. Furthermore, the mechanisms of action of LSB1 on S. argenteus were preliminarily explored. These results indicate that LSB1 might be potentially used as an effective and natural food preservative.

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“…Lactobacillus bacteria, through lactic acid production, are able to up-regulate the autophagy process through cyclic adenosine monophosphate (cAMP) inhibition, promoting pathogen bacteria clearing. In order to kill other bacteria and to prevent vaginal colonization by pathogen species, Lactobacillus bacteria synthesize hydrogen peroxide (H 2 O 2 ) and bacteriocins proteins [36,37], contributing to maintain a healthy genitourinary status. Many other bacterial genera were detected in healthy or not-healthy vaginal microbiota, including Actinomyces, Bacteroides, Campylobacter, Corynebacterium, Enterobacter, Escherichia, Gardnerella, Haemophilus, Salmonella, Shigella, Staphylococcus, Streptococcus, Ureaplasma [38,39] (Table 1).…”

Section: Microbial Colonization In Femalementioning

confidence: 99%

Human genital tracts microbiota: dysbiosis crucial for infertility

Venneri

Franceschini

Sciarra

et al. 2022

J Endocrinol Invest

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Human body is colonized by trillions of microbes, influenced by several factors, both endogenous, as hormones and circadian regulation, and exogenous as, life-style habits and nutrition. The alteration of such factors can lead to microbial dysbiosis, a phenomenon which, in turn, represents a risk factor in many different pathologies including cancer, diabetes, autoimmune and cardiovascular disease, and infertility. Female microbiota dysbiosis (vaginal, endometrial, placental) and male microbiota dysbiosis (seminal fluid) can influence the fertility, determining a detrimental impact on various conditions, as pre-term birth, neonatal illnesses, and macroscopic sperm parameters impairments. Furthermore, unprotected sexual intercourse creates a bacterial exchange between partners, and, in addition, each partner can influence the microbiota composition of partner’s reproductive tracts. This comprehensive overview of the effects of bacterial dysbiosis in both sexes and how partners might influence each other will allow for better personalization of infertility management.

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Characterization, modes of action, and application of a novel broad-spectrum bacteriocin BM1300 produced by Lactobacillus crustorum MN047

Cited by 27 publications

References 44 publications

Identification of Unique Quinazolone Thiazoles as Novel Structural Scaffolds for Potential Gram-Negative Bacterial Conquerors

Identification of Unique Quinazolone Thiazoles as Novel Structural Scaffolds for Potential Gram-Negative Bacterial Conquerors

Purification and characterization of Lactobacillus plantarum‐derived bacteriocin with activity against Staphylococcus argenteus planktonic cells and biofilm

Human genital tracts microbiota: dysbiosis crucial for infertility

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