CHARACTERIZATION IN THE FATTY ACID DISTRIBUTIONS OF TRIACYLGLYCEROLS AND PHOSPHOLIPIDS IN KIDNEY BEANS (<i>PHASEOLUS VULGARIS</i> L.)

Euro J Lipid Sci & Tech

Tanaka

et al. 2007

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Seed oils from four legume cultivars of Pisum sativum, grown in Japan, were extracted and classified by thin-layer chromatography (TLC) into seven fractions: hydrocarbons (HC; 0.5-0.9 wt-%), steryl esters (SE; 0.8-2.4 wt-%), triacylglycerols (TAG; 31.2-40.3 wt-%), free fatty acids (FFA; 1.3-2.7 wt-%), 1,3-diacylglycerols (1,3-DAG; 1.0-1.8 wt-%), 1,2-diacylglycerols (1,2-DAG; 1.0-2.2 wt-%) and phospholipids (PL; 52.2-61.3 wt-%). All lipid samples had high amounts of total unsaturated fatty acids, representing 75.0-84.3 wt-% for TAG and PL. Molecular species and fatty acid distributions of TAG, isolated from the total lipids in the peas, were analyzed by a combination of argentation-TLC and GC. Eighteen different molecular species were detected. With a few exceptions, the main TAG components were SMD (7.5-10.3 wt-%), M 2 D (8.0-8.9 wt-%), SD 2 (12.0-18.3 wt-%), SMT (9.8-11.0 wt-%), MD 2 (12.0-20.3 wt-%), SDT (9.7-10.8 wt-%), M 2 T (2.5-7.3 wt-%) and D 3 (14.5-15.2 wt-%) (where S denotes a saturated fatty acid, M denotes a monoene, D denotes a diene, and T denotes a triene). It seems that the four cultivars were highly related to each other based on the fatty acid composition of the TAG as well as the distribution profiles in the different TAG molecular species. In general, these results suggest that there are no essential differences (p .0.05) in the oil components among the four cultivars.

“…The extracted lipids were weighed to determine the lipid content of the peas and then transferred to a 25-mL brown glass volumetric flask with chloroform/methanol (2 : 1, vol/vol) [9].…”

Section: Extraction Of Lipidsmentioning

confidence: 99%

Characteristic profiles of lipid classes, fatty acids and triacylglycerol molecular species of peas (Pisum sativum L.)

Euro J Lipid Sci & Tech

Tanaka

et al. 2007

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“…3; Applied Science, State College, PA), using an electronic integrator (Shimadzu C-R6A). The other GC conditions were the same as described previously (12). The detection limit was 0.05% of total FA for each FAME in a FAME mixture, and results are expressed as the weight percentage (%) of total FAME.…”

Section: Methodsmentioning

confidence: 99%

“…The residue was dissolved in the mobile phase used for HPLC analysis (n-hexane/1,4-dioxane/ethanol, 490:10:1, by vol). The chromatographic system was the same as previously described (12) and operated at a flow rate of 2.0 mL/min. An aliquot (5-10 µL) of these solutions was injected with a fully loaded 20-µL loop onto the column.…”

Section: Methodsmentioning

confidence: 99%

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Tocopherol distribution and molecular species of triacylglycerols in soybean embryonic axes

Kanrei

et al. 2006

J Americ Oil Chem Soc

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Soybean embryonic axes were separated from other tissues, i.e., the cotyledons and seed coat. The molecular species and FA distribution of TAG isolated from total lipids in the embryonic axes were analyzed by a combination of argentation-TLC and GC, and were investigated in relation to their tocopherol distribution, which was determined by HPLC. The dominant components were γ-tocopherols, with much smaller amounts of α-, β-, and δ-tocopherols. A modified argentation-TLC procedure, developed to optimize the separation of the complex mixture of total TAG, provided 16 different groups of TAG, based on both the degree of unsaturation and the total acyl-chain length of FA groups. With a few exceptions, the major TAG components were S 2 D (6.8-10.DT 2 (8.2-15.7%) (where S denotes saturated FA, M denotes monoenes, D denotes dienes, and T denotes trienes). These results indicate that there were significant differences (P < 0.05) not only in tocopherol distribution but also in the molecular species of TAG among the four cultivars. Therefore, these tissues should be made available as raw materials for soybean-germ oil or soy milk, based on the differences in the distributions of tocopherol homologs and the molecular species of TAG within the embryonic axes.Paper no. J11245 in JAOCS 83, 345-351 (April 2006).

“…After phase separation, the chloroform layer was withdrawn, dried over anhydrous sodium sulfate and filtered, and the filtrate was concentrated under vacuum with a rotary evaporator at 35 7C. The extracted lipids were weighed to determine the lipid content of the beans and then transferred to a 25-mL brown-glass volumetric flask with chloroform/methanol (2 : 1, vol/vol) [17].…”

Section: Extraction Of Lipidsmentioning

confidence: 99%

Regiospecific distribution of fatty acids in triacylglycerols and phospholipids from broad beans (Vicia faba)

Euro J Lipid Sci & Tech

et al. 2009

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Regiospecific distributions of fatty acids of triacylglycerols (TAG) and phospholipids (PL) separated from broad beans (Vicia faba) of four cultivars (Minpo, Sanuki, Nintoku and Sanren) were investigated. The major lipid components were PL (47.5-50.5 wt-%) and TAG (47.7-50.1 wt-%), while steryl esters, hydrocarbons, free fatty acids, diacylglycerols and monoacylglycerols were present in minor proportions (1.6-2.4 wt-%). The PL components isolated from the four cultivars were phosphatidylcholine (56.4-58.4 wt-%), phosphatidylethanolamine (20.3-21.7 wt-%) and phosphatidylinositol (16.6-18.6 wt-%). Phosphatidylinositol was unique in that it had the highest saturated fatty acid content among these PL. The principal characteristics of the fatty acid distribution in the TAG and PL were evident in the beans: Unsaturated fatty acids were predominantly concentrated in the sn-2 position while saturated fatty acids primarily occupied the sn-1 or sn-3 position in these lipids. The lipid components and fatty acid distributions were almost the same in the four cultivars and were not influenced by genetic variability and planting location. These results could be useful information to both consumers and producers for the manufacture of traditional broad bean foods in Japan.