Characterization, hypoglycemia and antioxidant activities of polysaccharides from Rhodosorus sp. SCSIO-45730

Wang, Na; Chen, Zishuo; Lv, Jinting; Li, Tao; Wu, Hao; Wu, Jiayi; Wu, Houbo; Xiang, Wenzhou

doi:10.1016/j.indcrop.2022.115936

Cited by 12 publications

(5 citation statements)

References 55 publications

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“…The Congo red test can be used to determine triple‐helical conformation in polysaccharides [32,33] . Polysaccharides with a triple‐helical conformation can form complexes with Congo red in alkaline solutions, resulting in a red shift of the absorption wavelength maximum (λ max ) [5] .…”

Section: Resultsmentioning

confidence: 99%

“…The Congo red test can be used to determine triple-helical conformation in polysaccharides. [32,33] Polysaccharides with a triple-helical conformation can form complexes with Congo red in alkaline solutions, resulting in a red shift of the absorption wavelength maximum (λ max ). [5] As shown in Figure 1C, RFCP evaluated at different concentrations of sodium hydroxide in the presence of Congo red, did not exhibit a significant shift of the λ max compared to the control (Congo red).…”

Section: Congo Red Assaymentioning

confidence: 99%

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Antioxidant and cytotoxic properties of water‐soluble crude polysaccharides from two edible mushroom species of Ramaria

Muñoz‐Castiblanco,

Santa Maria de la Parra,

Peña‐Cañón

et al. 2024

ChemistrySelect

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Water‐soluble crude polysaccharides (WSCPs) were isolated from the fruiting bodies of Ramaria flava (Schaeff.) Quél. (RFCP) and Ramaria cf. aurea (Schaeff.) Quél. (RACP). Species of Ramaria were collected in Boyacá‐Colombia. WSCPs were obtained by hot‐water extraction, ethanol precipitation, and dialysis and characterized using UV‐Vis spectroscopy, gel permeation chromatography, high‐performance liquid chromatography‐diode array detector, fourier transform infrared and nuclear magnetic resonance spectroscopy. Different assays evaluated the antioxidant capacity, and the 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT)‐assay was used to determine cytotoxic effects. Chemical compositional analysis revealed that RFCP and RACP are heteropolysaccharides with molecular weights of 407 and 818 kDa, respectively compose mainly of glucose, mannose, galactose, fucose, and glucuronic acid with small amounts of protein and polyphenols. The backbone of RFCP and RACP is composed of β‐D‐Glcp‐(4→,→3)‐β‐D‐Glcp‐(1→,→2,6)‐α‐D‐Galp‐(1→,→3,6)‐α‐D‐Manp‐(1→. Antioxidant capacity demonstrated that both crude polysaccharides exhibit reducing power, radical scavenging ability, and a protective effect on lipid‐peroxidation. MTT assay showed that RFCP and RACP inhibited the growth of cancer cells HCT 116‐(colorectal) and MG‐63‐(bone) in a concentration‐dependent manner. Compared with RFCP, RACP showed better antioxidant capacity and cytotoxic effects due to differences in composition, molecular weight, and triple‐helical conformation. Overall, WSCPs exhibited a promising biological effect, highlighting the cultural, nutritional, and medicinal value of wild mushrooms.

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Section: Resultsmentioning

confidence: 99%

Section: Congo Red Assaymentioning

confidence: 99%

Antioxidant and cytotoxic properties of water‐soluble crude polysaccharides from two edible mushroom species of Ramaria

Muñoz‐Castiblanco,

Santa Maria de la Parra,

Peña‐Cañón

et al. 2024

ChemistrySelect

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“…where Y is the polysaccharide extraction rate of U. lactuca (%), b 0 , b i , b ii and b ij are the regression coefficients and X i and X j are the coded independent factors. The extracted crude polysaccharide was purified on cellulose DEAE and Sephadex G-100 columns, and its antioxidant activity was determined [24]. In total, 2 mM DPPHethanol solution was mixed with the samples in a 1:1 volume ratio, protected from light for 30 min, and the absorbance at 517 nm was measured using a microplate reader (SpectraMax i3X, Molecular Devices, Shanghai, China) [25].…”

Section: Optimization Of Polysaccharide Extraction Conditionsmentioning

confidence: 99%

Ultrasound-Assisted Multi-Enzyme Extraction for Highly Efficient Extraction of Polysaccharides from Ulva lactuca

Wang,

Li,

et al. 2024

Foods

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Ulva polysaccharides present several physiological activities including antiviral, antitumor and anti-plasmodial effects. However, current processing usually results in low yields and high prices, thus lacking commercialization potential. The aim of this study was to develop an efficient method for the extraction of Ulva polysaccharides with high biological activity. The effect of cell wall-degrading enzymes including cellulase, hemicellulase, pectinase and protease on Ulva polysaccharide extraction was studied by statistical mixing design. Using the most effective enzyme preparations as the basic components, the optimal proportions of the enzyme mixture were determined as follows: cellulase 35.3%, pectinase 34.5%, alkaline protease 30.2%, which increased the polysaccharide yield from 6.43% in the absence of enzymes to 26.68%. Subsequently, through response surface analysis, the optimal conditions were determined: enzyme concentration of 1.5%, enzymatic time of 1.1 h, ultrasonic time of 90 min and enzymatic temperature of 60 °C. Under the optimal extraction conditions, the extraction yield of Ulva polysaccharides could be increased to 30.14%. Moreover, extracted polysaccharides exhibit strong antioxidant properties in DPPH, ABTS, hydroxyl radical, superoxide radical and H2O2-induced cellular damage models. This study laid a solid foundation for the use and development of Ulva polysaccharides.

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“…SCSIO-45730 polysaccharides (RSP) fractions were called RSP-1, PSR-2 and RSP-3, respectively. Among them, RSP-1 and RSP-3 exhibited marked antioxidant activities, and RSP-2 demonstrated strong hypoglycemia activity (Wang et al, 2022a). In addition, the biomass of Rhodosorus sp.…”

Section: Introductionmentioning

confidence: 99%

Effects of seawater acidification and solar ultraviolet radiation on photosynthetic performances and biochemical compositions of Rhodosorus sp. SCSIO-45730

Wang

Yang

et al. 2022

Front. Mar. Sci.

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Ocean acidification (OA) caused by rising atmospheric CO2 concentration and solar ultraviolet radiation (UVR) resulting from ozone depletion may affect marine organisms, but little is known regarding how unicellular Rhodosorus sp. SCSIO-45730, an excellent species resource containing various biological-active compounds, responds to OA and UVR. Therefore, we conducted a factorial coupling experiment to unravel the combined effects of OA and UVR on the growth, photosynthetic performances, biochemical compositions and enzyme activities of Rhodosorus sp. SCSIO-45730, which were exposed to two levels of CO2 (LC, 400 μatm, current CO2 level; HC, 1000 μatm, future CO2 level) and three levels of UVR (photosynthetically active radiation (PAR), PAR plus UVA, PAR plus UVB) treatments in all combinations, respectively. Compared to LC treatment, HC stimulated the relative growth rate (RGR) due to higher optimum and effective quantum yields, photosynthetic efficiency, maximum electron transport rates and photosynthetic pigments contents regardless of UVR. However, the presence of UVA had no significant effect but UVB markedly reduced the RGR. Additionally, higher carbohydrate content and lower protein and lipid contents were observed when Rhodosorus sp. SCSIO-45730 was cultured under HC due to the ample HCO3− applications and active stimulation of metabolic enzymes of carbonic anhydrase and nitrate reductase, thus resulting in higher TC/TN. OA also triggered the production of reactive oxygen species (ROS), and the increase of ROS coincided approximately with superoxide dismutase and catalase activities, as well as phenols contents. However, UVR induced photochemical inhibition and damaged macromolecules, making algal cells need more energy for self-protection. Generally, these results revealed that OA counteracted UVR-related inhibition on Rhodosorus sp. SCSIO-45730, adding our understanding of the red algae responding to future global climate changes.

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Characterization, hypoglycemia and antioxidant activities of polysaccharides from Rhodosorus sp. SCSIO-45730

Cited by 12 publications

References 55 publications

Antioxidant and cytotoxic properties of water‐soluble crude polysaccharides from two edible mushroom species of Ramaria

Antioxidant and cytotoxic properties of water‐soluble crude polysaccharides from two edible mushroom species of Ramaria

Ultrasound-Assisted Multi-Enzyme Extraction for Highly Efficient Extraction of Polysaccharides from Ulva lactuca

Effects of seawater acidification and solar ultraviolet radiation on photosynthetic performances and biochemical compositions of Rhodosorus sp. SCSIO-45730

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