Characterization, expression and localization of valosin-containing protein in ovaries of the giant tiger shrimp Penaeus monodon

Talakhun, Witchulada; Khamnamtong, Bavornlak; Nounurai, Pachumporn; Klinbunga, Sirawut; Menasveta, Piamsak

doi:10.1016/j.gene.2013.09.089

Cited by 7 publications

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“…The first step toward understanding molecular mechanisms of reproduction is to identify and characterize sex-related genes and regulatory pathways. Many efforts have been made to reveal sex-related genes, and many of these genes are cloned and characterized in shrimp; for example, M-phase phosphoprotein 6 ( MPP6 ) [ 10 ], cell division cycle 2 ( Cdc2 ) [ 11 ], cyclin A and cyclin B [ 12 ], gonad-inhibiting hormone ( GIH ) [ 13 ], mitogen-activating protein kinase 1 ( MAPK1 ) [ 14 ], prostaglandin reductase 1 [ 15 ], and valosin-containing protein ( VCP ) [ 16 ] in Penaeus monodon ; and sex-determiner transformer-2 ( Tra-2 ) [ 17 ], activated protein kinase C1 ( RACK1 ) [ 18 ], and cell apoptosis susceptibility ( FcCAS ) [ 19 ] in Chinese shrimp Fenneropenaeus chinensis . In L. vannamei , vasa-like , vitellin , gonadotropin-releasing hormone-like and a sex-related marker have been identified [ 20 – 23 ].…”

Section: Introductionmentioning

confidence: 99%

Gonadal transcriptomic analysis and differentially expressed genes in the testis and ovary of the Pacific white shrimp (Litopenaeus vannamei)

et al. 2015

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BackgroundThe Pacific white shrimp (Litopenaeus vannamei) is the world’s most prevalent cultured crustacean species. However, the supply of high-quality broodstocks is limited and baseline information related to its reproductive activity and molecular issues related to gonad development are scarce. In this study, we performed transcriptome sequencing on the gonads of adult male and female L. vannamei to identify sex-related genes.ResultsA total of 25.16 gigabases (Gb) of sequences were generated from four L. vannamei gonadal tissue libraries. After quality control, 24.11 Gb of clean reads were selected from the gonadal libraries. De-novo assembly of all the clean reads generated a total of 65,218 unigenes with a mean size of 1021 bp and a N50 of 2000 bp. A search of all-unigene against Nr, SwissProt, KEGG, COG and NT databases resulted in 26,482, 23,062, 20,659, 11,935 and 14,626 annotations, respectively, providing a total of 30,304 annotated unigenes. Among annotated unigenes, 12,320 unigenes were assigned to gene ontology categories and 20,659 unigenes were mapped to 258 KEGG pathways. By comparing the ovary and testis libraries, 19,279 testicular up-regulated and 3,529 ovarian up-regulated unigenes were identified. Enrichment analysis of differentially expressed unigenes resulted in 1060 significantly enriched GO terms and 34 significantly enriched KEGG pathways. Nine ovary-specific, 6 testis-specific, 45 testicular up-regulated and 39 ovarian up-regulated unigenes were then confirmed by semi-quantitative PCR and quantitative real-time PCR. In addition, using all-unigenes as a reference, a total of 13,233 simple sequence repeats (SSRs) were identified in 10,411 unigene sequences.ConclusionsThe present study depicts the first large-scale RNA sequencing of shrimp gonads. We have identified many important sex-related functional genes, GO terms and pathways, all of which will facilitate future research into the reproductive biology of shrimp. We expect that the SSRs detected in this study can then be used as genetic markers for germplasm evaluation of breeding and imported populations.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-015-2219-4) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Gonadal transcriptomic analysis and differentially expressed genes in the testis and ovary of the Pacific white shrimp (Litopenaeus vannamei)

et al. 2015

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“…Several methods, such as traditional suppression, subtractive hybridisation, proteomic analysis, expressed sequence tag sequencing and microarray sequencing, have already been utilized to identify potential sex‐related genes in shrimps (Cui et al, 2014; Karoonuthaisiri et al, 2009; Leelatanawit et al, 2008; Talakhun, Phaonakrop, et al, 2014). Furthermore, several genes have already been identified, such as M‐phase phosphoprotein 6 , cell division cycle 2 , cyclin A and cyclin B , gonad‐inhibiting hormone , mitogen‐activating protein kinase 1 , prostaglandin reductase 1 , valosin‐containing protein , sex‐determiner transformer‐2 and activated protein kinase C1 (Phinyo et al, 2013; Talakhun, Khamnamtong et al, 2014; Talakhun, Phaonakrop, et al, 2014; Treerattrakool et al, 2008; Visudtiphole et al, 2009; Zhou et al, 2013). Cell apoptosis susceptibility (Wen et al, 2012) was also identified in Fenneropenaeus chinensis and in Litopenaeus vannamei , vasa‐like genes, vitellin, gonadotropin‐releasing hormones and a sex‐related marker were identified (Tinikul et al, 2011; Wen et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Identification of gonadal associated genes in black tiger shrimp ( Penaeus monodon ) using transcriptome analysis and high‐throughput sequencing

Jiang

et al. 2022

Aquaculture Research

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Black tiger shrimp (Penaeus monodon) is a commercially important species. It is reported to have low reproductive maturation rates in captivity. To improve the production of this species via sustainable aquaculture, genetic techniques are required to improve its maturation rates. However, to date, reports on P. monodon reproduction at a transcriptional level are limited. To address this, we conducted transcriptome sequencing of the ovaries and testes of P. monodon using high-throughput sequencing to identify sex-related pathways and genes. A total of 75.74 Gb of clean data were generated from nine gonadal tissue libraries. We obtained 62,276 unigenes with a mean length of 1452 bp and N50 of 2423 bp. A total of 11,411 significantly differentially expressed genes (DEGs) were identified between the testis and developed ovaries. Enrichment analysis of the DEGs identified 415 significantly enriched GO terms and 106 enriched KEGG pathways. Genes related to the P. monodon ovaries were enriched in the steroid biosynthesis, mRNA surveillance, nucleocytoplasmic transport, oxidative phosphorylation and DNA replication pathways. Five upregulated and five downregulated ovarian genes were confirmed using RT-PCR. This investigation has thus identified important sex-related functional genes, GO terms and pathways in P. monodon. In particular, we identified several important genes and pathways related to gonadal development, such as GnRH/GnRHR-like, Hsp90, PCNA, cathepsin L, Wnt signalling pathway and ovarian steroidogenesis pathway, which provide important insights into the mechanism analysis and sex regulation of shrimp gonadal development. The results will provide reference data for future research into the reproductive biology of shrimp, which will aid in improved black tiger shrimp breeding in captivity.

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Characterization and expression analysis of Cyclin-dependent kinase 7 gene and protein in ovaries of the giant tiger shrimp Penaeus monodon

et al. 2014

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Characterization, expression and localization of valosin-containing protein in ovaries of the giant tiger shrimp Penaeus monodon

Cited by 7 publications

References 58 publications

Gonadal transcriptomic analysis and differentially expressed genes in the testis and ovary of the Pacific white shrimp (Litopenaeus vannamei)

Gonadal transcriptomic analysis and differentially expressed genes in the testis and ovary of the Pacific white shrimp (Litopenaeus vannamei)

Identification of gonadal associated genes in black tiger shrimp ( Penaeus monodon ) using transcriptome analysis and high‐throughput sequencing

Characterization and expression analysis of Cyclin-dependent kinase 7 gene and protein in ovaries of the giant tiger shrimp Penaeus monodon

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