Characterization, Expression, and Functional Analysis of a Novel NAC Gene Associated with Resistance to Verticillium Wilt and Abiotic Stress in Cotton

Wang, Weina; Yuán, Yǒulù; Yang, Chunrong; Geng, Shuaipeng; Sun, Quan; Long, Lu; Cai, Chaowei; Chu, Zongyan; Liu, Xin; Wang, Guanghao; Du, Xiongming; Miao, Chen; Zhang, Xiao; Cai, Yingfan

doi:10.1534/g3.116.034512

Cited by 32 publications

(17 citation statements)

References 46 publications

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“…Another component of the stress-induced NRP/DCD-mediated signaling, GmNAC081 (Glyma.12G022700; Faria et al, 2011), was clustered in the TERN (Tobacco elicitor-responsive gene encoding NAC-domain protein) subfamily, which encompasses functionally similar NAC proteins that are involved in resistance to biotic and abiotic stresses. Examples from this subfamily include (i) Gossypium barbadense (Gb)NAC1, which is involved in the positive regulation of resistance to Verticillium and in abiotic stress response in cotton (Wang et al, 2016), (ii) the chitin-responsive gene AT3G44350 (ANAC061 ) and (iii) AT2G17040 ( ANAC036) , an orthologous gene of GmNAC081 (Libault et al, 2007; Reis et al, 2016). GmNAC081 has been shown to regulate stress-induced and developmentally programmed leaf senescence (Mendes et al, 2013; Pimenta et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Revisiting the Soybean GmNAC Superfamily

et al. 2018

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The NAC (NAM, ATAF, and CUC) genes encode transcription factors involved with the control of plant morph-physiology and stress responses. The release of the last soybean (Glycine max) genome assembly (Wm82.a2.v1) raised the possibility that new NAC genes would be present in the soybean genome. Here, we interrogated the last version of the soybean genome against a conserved NAC domain structure. Our analysis identified 32 putative novel NAC genes, updating the superfamily to 180 gene members. We also organized the genes in 15 phylogenetic subfamilies, which showed a perfect correlation among sequence conservation, expression profile, and function of orthologous Arabidopsis thaliana genes and NAC soybean genes. To validate our in silico analyses, we monitored the stress-mediated gene expression profiles of eight new NAC-genes by qRT-PCR and monitored the GmNAC senescence-associated genes by RNA-seq. Among ER stress, osmotic stress and salicylic acid treatment, all the novel tested GmNAC genes responded to at least one type of stress, displaying a complex expression profile under different kinetics and extension of the response. Furthermore, we showed that 40% of the GmNACs were differentially regulated by natural leaf senescence, including eight (8) newly identified GmNACs. The developmental and stress-responsive expression profiles of the novel NAC genes fitted perfectly with their phylogenetic subfamily. Finally, we examined two uncharacterized senescence-associated proteins, GmNAC065 and GmNAC085, and a novel, previously unidentified, NAC protein, GmNAC177, and showed that they are nuclear localized, and except for GmNAC065, they display transactivation activity in yeast. Consistent with a role in leaf senescence, transient expression of GmNAC065 and GmNAC085 induces the appearance of hallmarks of leaf senescence, including chlorophyll loss, leaf yellowing, lipid peroxidation and accumulation of H2O2. GmNAC177 was clustered to an uncharacterized subfamily but in close proximity to the TIP subfamily. Accordingly, it was rapidly induced by ER stress and by salicylic acid under late kinetic response and promoted cell death in planta. Collectively, our data further substantiated the notion that the GmNAC genes display functional and expression profiles consistent with their phylogenetic relatedness and established a complete framework of the soybean NAC superfamily as a foundation for future analyses.

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Section: Discussionmentioning

confidence: 99%

Revisiting the Soybean GmNAC Superfamily

et al. 2018

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“…1), in Nicotiana benthamiana increased susceptibility to Phytophthora infestans infection (McLellan et al 2013). GbNAC1-silenced cotton plants attenuated resistance to Verticillium dahlia while GbNA-C1-overexpressing Arabidopsis plants enhanced resistance to V. dahliae (Wang et al 2016b). Overexpression of grapevine VvNAC1 or Artemisia annua AaNAC1 in Arabidopsis or tobacco plants enhanced resistance to B. cinerea or P. parasitica var.…”

Section: Functions Of Nac Tfs In Plant Immunity Against Necrotrophic mentioning

confidence: 99%

NAC transcription factors in plant immunity

et al. 2019

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The NAC (NAM, ATAF and CUC) family is one of the largest plant-specific transcription factor (TF) families. Members of this family are implicated in plant growth, development and stress responses. Recent functional studies demonstrate that a number of NAC TFs function as positive or negative regulators of plant immunity to biotrophic, hemibiotrophic or necotrophic pathogens, as modulators of the hypersensitive responses and stomatal immunity or as virulence targets of pathogen effectors. They affect plant immunity through their regulatory impact on signaling of plant hormones, which in turn are key players in plant immune responses. This review summarizes current knowledge and recent progress in our understanding of the biological functions of NAC TFs in plant immunity and discusses perspectives and directions for further study to elucidate the molecular mechanisms of NAC TF functions in immunity and potential application in improvement of crop disease resistance.

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“…Recently, a NAC gene GbNAC1 was identified from G. barbadense . Overexpression of GbNAC1 in Arabidopsis can significantly enhance resistance to VW, implying that G. barbadense NAC genes might play pivotal roles in biotic stress resistance (Wang et al, 2016). The availability of diploid and tetraploid cotton genomes has allowed scientists to identify the NAC gene family members at the genome-wide scale, such as 145 genes in G. raimondii (Shang et al, 2013), 141 in G. arboreum (Shang et al, 2016; Fan et al, 2018), and 283 in G. hirsutum (Sun et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Systematic analysis of NAC transcription factors in Gossypium barbadense uncovers their roles in response to Verticillium wilt

Liu

et al. 2019

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As one of the largest plant-specific gene families, the NAC transcription factor gene family plays important roles in various plant physiological processes that are related to plant development, hormone signaling, and biotic and abiotic stresses. However, systematic investigation of the NAC gene family in sea-island cotton (Gossypium babardense L.) has not been reported, to date. The recent release of the complete genome sequence of sea-island cotton allowed us to perform systematic analyses of G. babardense NAC GbNAC) genes. In this study, we performed a genome-wide survey and identified 270 GbNAC genes in the sea-island cotton genome. Genome mapping analysis showed that GbNAC genes were unevenly distributed on 26 chromosomes. Through phylogenetic analyses of GbNACs along with their Arabidopsis counterparts, these proteins were divided into 10 groups (I–X), and each contained a different number of GbNACs with a similar gene structure and conserved motifs. One hundred and fifty-four duplicated gene pairs were identified, and almost all of them exhibited strong purifying selection during evolution. In addition, various cis-acting regulatory elements in GbNAC genes were found to be related to major hormones, defense and stress responses. Notably, transcriptome data analyses unveiled the expression profiles of 62 GbNAC genes under Verticillium wilt (VW) stress. Furthermore, the expression profiles of 15 GbNAC genes tested by quantitative real-time PCR (qPCR) demonstrated that they were sensitive to methyl jasmonate (MeJA) and salicylic acid (SA) treatments and that they could be involved in pathogen-related hormone regulation. Taken together, the genome-wide identification and expression profiling pave new avenues for systematic functional analysis of GbNAC candidates, which may be useful for improving cotton defense against VW.

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Characterization, Expression, and Functional Analysis of a Novel NAC Gene Associated with Resistance to Verticillium Wilt and Abiotic Stress in Cotton

Cited by 32 publications

References 46 publications

Revisiting the Soybean GmNAC Superfamily

Revisiting the Soybean GmNAC Superfamily

NAC transcription factors in plant immunity

Systematic analysis of NAC transcription factors in Gossypium barbadense uncovers their roles in response to Verticillium wilt

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