Characterization, expression, and function analysis of AKR1A1 gene from yellow catfish (Tachysurus fulvidraco)

Swamp eel aldehyde reductase is involved in response to nitrosative stress via regulating NO/GSH levels

“…ALR is an important carbonyl detoxification enzyme in organisms (Yang et al ., 2022) that can reduce endogenous and exogenous aldehydes to corresponding alcohols in an NADPH‐dependent manner (Penning, 2017). In the present study, the authors cloned ALR of the swamp eel that was shown by sequence alignment to be similar to that of other teleosts.…”

“…Bacteria were cultured at 28°C until the mid‐logarithmic growth phase and then diluted in normal saline (0.9%) to a concentration of 10 6 CFU ml −1 . rALR was administered according to methods described by (Yang et al ., 2022). To avoid oxidative damage caused by a high dose of rALR, a medium dose of protein was intraperitoneally injected.…”

“…Western blot analysis of swamp eel rALR was performed as described by Yang et al . (2022) with slight modifications. SDS‐PAGE electrophoresis was performed, and the recombinant rALR was transferred to a nitrocellulose membrane.…”

“…Aldehyde reductase activity of rALR was determined following the procedure described by Yang (Yang et al ., 2022). The reaction system consisted of 1.9 ml of PBS (50 mmol l −1 ), 1 ml of substrate (40 mmol l −1 ), 90 μl of rALR and 10 μl of NADPH (10.0 mmol l −1 ).…”

“…In zebrafish, ALR is vital for maintaining glucose balance (Li et al ., 2020), whereas ALR is involved in the detoxification of benzo (a) pyrene in tilapia (Hassanin et al ., 2017; Osorio‐Yáñez et al ., 2012). Administration of recombinant ALR alleviated oxidative damage and reduced immunotoxicity in yellow catfish under cadmium stress (Yang et al ., 2022). Nonetheless, the GSNO reductive activity of ALRs in teleosts has not yet been documented.…”