Characterization, cloning and immunolocalization of a coronin homologue in Trichomonas vaginalis

Bricheux, Geneviève; Coffe, Gérard; Bayle, Danielle; Brugerolle, Guy

doi:10.1078/0171-9335-00065

Cited by 24 publications

(13 citation statements)

References 44 publications

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“…In agreement with previous reports (Francioli et al, 1983; Juliano et al, 1987, 1991), the present results also demonstrated that cytochalasin D treatment was effective in reducing the ability of both the JT and T016 isolates to phagocytose yeast cells. In agreement with previous studies (Brugerolle et al, 1996; Bricheux et al, 2000), it was also demonstrated that fibrillar actin is present in the whole cytoplasm, but more concentrated in the periphery and close to the surface of these cells. However, when parasites were treated with cytochalasin D and incubated with fluorescent phalloidin or anti‐actin antibody, a reduced labelling intensity was observed.…”

Section: Discussionsupporting

confidence: 92%

Phagocytosis by Trichomonas vaginalis: new insights

Pereira-Neves

Benchimol

2007

Biology of the Cell

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Background information. The parasitic protozoan Trichomonas vaginalis is the causative agent of trichomoniasis, a sexually transmitted disease. The phagocytic activity of this parasite has not been completely elucidated. In order to better understand the mechanisms of trichomonal phagocytosis, we have studied the in vitro capacity of T. vaginalis to phagocytose and degrade Saccharomyces cerevisiae cells. Results and conclusions.To analyse the phagocytic ability and capacity, two isolates of T. vaginalis presenting different virulence grades were used. Complementary techniques, such as fluorescence microscopy, computerbased fluorescence analysis, scanning and transmission electron microscopy and the use of drugs that interfere with the actin microfilaments, were used in order to follow the behaviour of the actin cytoskeleton during phagocytosis of yeast cells by T. vaginalis. It was concluded that: (1) T. vaginalis changes its shape rapidly and engulfs the yeast cells, which are almost as large as the parasite; (2) long-term and fresh cultures are able to phagocytose, although the low-virulence strain JT demonstrated a lower activity when compared with the highly virulent T016 isolate; (3) the T016 strain exhibited an amoeboid morphology during the internalization of yeast cells in contrast with the JT strain; (4) attachment of yeast cells to the parasite occurs via the whole cell surface, including both anterior and recurrent flagella; (5) two forms of phagocytosis were observed: a 'sinking' process without any apparent participation of plasma membrane extensions and the classical phagocytosis where pseudopodia are extended toward the target cell; (6) the internalized S. cerevisiae are digested in lysosomes; (7) competitor sugars D-mannose or L-fucose inhibit the phagocytosis, and inhibition was 1.67 times higher in long-term cultured JT than that of the parasites from fresh isolate T016; (8) a thick layer of actin microfilaments was present underlying the plasma membrane, and especially in the pseudopodia and around the phagocytosed particles; (9) a dramatic change in the distribution pattern of fibrillar actin occurred during phagocytosis; (10) cytochalasin D depressed the phagocytosis; (11) a non-specific recognition and phagocytosis of yeast cells by T. vaginalis is mediated by a mannose receptor present on the parasite surface; (12) the phagocytic process may occur simultaneously during mitosis of the parasite.

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Section: Discussionsupporting

confidence: 92%

Phagocytosis by Trichomonas vaginalis: new insights

Pereira-Neves

Benchimol

2007

Biology of the Cell

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“…While this pattern is also valid for genes such as ␣-actinin or the Arp2/3 subunits, it does not apply to all genes associated with the actin cytoskeleton: profilin, the actin family itself and coronin are upregulated upon infection [14]. For the latter empirical evidence exists, too, that it is involved in morphogenesis [69]. While the actin machinery mediates amoeboid movement, it apparently functions without the normally associated motor protein myosin, for which no homologs are encoded by T. vaginalis [10].…”

Section: Morphogenesismentioning

confidence: 79%

The biology of Trichomonas vaginalis in the light of urogenital tract infection

Kusdian

Gould

2014

Molecular and Biochemical Parasitology

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“…Assuming that the driving force for host cell entry involves polymerization of parasite actin and its AIPs, plus the recognized subcellular localization of functional coronins and Arps shown in the phagosome (Bricheux et al , 2000; Asano et al , 2001; Baldo et al , 2005), then we can propose a possible viable role for flagellar coronin and Arp2/3 within phagosome. As Love et al (1998) have shown, periphagosomal actin is rapidly lost when parasites are internalized.…”

Section: Discussionmentioning

confidence: 99%

Actin-interacting and flagellar proteins in Leishmania spp.: bioinformatics predictions to functional assignments in phagosome formation

et al. 2009

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Several motile processes are responsible for the movement of proteins into and within the flagellar membrane, but little is known about the process by which specific proteins (either actin-associated or not) are targeted to protozoan flagellar membranes. Actin is a major cytoskeleton protein, while polymerization and depolymerization of parasite actin and actin-interacting proteins (AIPs) during both processes of motility and host cell entry might be key events for successful infection. For a better understanding the eukaryotic flagellar dynamics, we have surveyed genomes, transcriptomes and proteomes of pathogenic Leishmania spp. to identify pertinent genes/proteins and to build in silico models to properly address their putative roles in trypanosomatid virulence. In a search for AIPs involved in flagellar activities, we applied computational biology and proteomic tools to infer from the biological meaning of coronins and Arp2/3, two important elements in phagosome formation after parasite phagocytosis by macrophages. Results presented here provide the first report of Leishmania coronin and Arp2/3 as flagellar proteins that also might be involved in phagosome formation through actin polymerization within the flagellar environment. This is an issue worthy of further in vitro examination that remains now as a direct, positive bioinformatics-derived inference to be presented.

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Characterization, cloning and immunolocalization of a coronin homologue in Trichomonas vaginalis

Cited by 24 publications

References 44 publications

Phagocytosis by Trichomonas vaginalis: new insights

Phagocytosis by Trichomonas vaginalis: new insights

The biology of Trichomonas vaginalis in the light of urogenital tract infection

Actin-interacting and flagellar proteins in Leishmania spp.: bioinformatics predictions to functional assignments in phagosome formation

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