Characterization by Polyacrylamide Gel Electrophoresis of Whole-Cell Proteins of Some Bacillus thuringiensis subsp. israelensis Strains Isolated in Brazil

Kaji, Denise A.; Rosato, Yoko Bomura; Canhos, Vanderlei Perez; Priest, Fergus G.

doi:10.1016/s0723-2020(11)80037-8

Cited by 12 publications

(5 citation statements)

References 15 publications

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“…However, there is evidence that the ST designation may relate more to insect toxicity than does the serovar. For example, B. thuringiensis serovar israelensis (ST-16) constitutes a large, globally widespread clone of highly active, mosquito-pathogenic strains with similar or identical crystal proteins (Cry4Aa, Cry4Ba, Cry10Aa, and Cry11Aa) (1,27). Interestingly, the only strain of B. thuringiensis serovar morrisoni included in ST-16 is also a dipteran pathogen and contains Cry4 toxins (data not shown), making this ST exclusive to Cry4-containing mosquito pathogens.…”

Section: Discussionmentioning

confidence: 99%

Population Structure and Evolution of the Bacillus cereus Group

Priest¹,

Barker²,

Baillie³

et al. 2004

J Bacteriol

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342

395

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Representative strains of the Bacillus cereus group of bacteria, including Bacillus anthracis (11 isolates), B. cereus (38 isolates), Bacillus mycoides (1 isolate), Bacillus thuringiensis (53 isolates from 17 serovars), and Bacillus weihenstephanensis (2 isolates) were assigned to 59 sequence types (STs) derived from the nucleotide sequences of seven alleles, glpF, gmk, ilvD, pta, pur, pycA, and tpi. Comparisons of the maximum likelihood (ML) tree of the concatenated sequences with individual gene trees showed more congruence than expected by chance, indicating a generally clonal structure to the population. The STs followed two major lines of descent. Clade 1 comprised B. anthracis strains, numerous B. cereus strains, and rare B. thuringiensis strains, while clade 2 included the majority of the B. thuringiensis strains together with some B. cereus strains. Other species were allocated to a third, heterogeneous clade. The ML trees and split decomposition analysis were used to assign STs to eight lineages within clades 1 and 2. These lineages were defined by bootstrap analysis and by a preponderance of fixed differences over shared polymorphisms among the STs. Lineages were named with reference to existing designations: Anthracis, Cereus I, Cereus II, Cereus III, Kurstaki, Sotto, Thuringiensis, and Tolworthi. Strains from some B. thuringiensis serovars were wholly or largely assigned to a single ST, for example, serovar aizawai isolates were assigned to ST-15, serovar kenyae isolates were assigned to ST-13, and serovar tolworthi isolates were assigned to ST-23, while other serovars, such as serovar canadensis, were genetically heterogeneous. We suggest a revision of the nomenclature in which the lineage and clone are recognized through name and ST designations in accordance with the clonal structure of the population.

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Section: Discussionmentioning

confidence: 99%

Population Structure and Evolution of the Bacillus cereus Group

Priest¹,

Barker²,

Baillie³

et al. 2004

J Bacteriol

Self Cite

342

395

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“…The traditional typing scheme based on flagellar (H) antigens has resulted in the description of 82 serotypes which are given serovar names [4]. While some serotypes such as H14 (serovar israelensis ) correlate well with toxicity, all serovar israelensis strains contain Cry4 proteins [5,6], other serovars such as serovar morrisoni include strains toxic for Coleoptera (pathovar tenebrionis ), Diptera or Lepidoptera each of which require different Cry proteins [7]. Conversely, strains from different serovars may have the same complement of Cry proteins [8].…”

Section: Introductionmentioning

confidence: 99%

Pulsed field gel electrophoresis of chromosomal DNA reveals a clonal population structure toBacillus thuringiensisthat relates in general to crystal protein gene content

Rivera

Priest

2003

FEMS Microbiology Letters

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Seventy strains of Bacillus thuringiensis representing 21 serovars were allocated to 38 genomic groups using pulsed field gel electrophoresis (PFGE) of restriction enzyme-digested DNA. There was a broad correlation between PFGE type and serotype for serovars darmstadiensis, israelensis, kenyae, kumamotoensis, kurstaki, sotto, thuringiensis, and tolworthi, although some serovars included atypical strains. Serovars canadensis and entomocidus were heterogeneous. Detection of crystal protein genes by polymerase chain reaction indicated an approximate correlation between PFGE type and cry gene complement. For example, cry1 products were amplified from DNA from PFGE type 17 strains of serovar aizawai and from PFGE type 23 strains of serovar tolworthi but not from a PFGE 18 strain of aizawai nor from a PFGE type 24 strain of tolworthi. These data suggest a clonal population structure to B. thuringiensis with some consistency of Cry-plasmid composition within PFGE types.

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“…1996). The good correlation between whole‐cell protein profiles and DNA–DNA relatedness, has certainly stimulated application of the method in order to compare a high number of bacterial strains (Kaji et al . 1994; Vandamme et al .…”

Section: Introductionmentioning

confidence: 99%

Properties of Bacillus thuringiensis isolated from bank voles

Święcicka

Vos

2003

J Appl Microbiol

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Aims: To assess the properties of B. thuringiensis naturally occurring in the intestines of bank voles. Methods and Results: Seventeen Bacillus thuringiensis strains, exhibiting typical growth on selective medium for the B. cereus group and characterized by the ability to produce parasporal crystals, were isolated from bank voles trapped in the Łom_ z za Landscape Park of the Narew River Valley (north-east Poland). All isolates were characterized by pulsed field gel electrophoresis (PFGE) of chromosomal DNA and SDS polyacrylamide gel electrophoresis (SDS-PAGE) of whole-cell proteins. Six pulsotypes were found with PFGE typing, using SmaI or NotI as restriction enzymes. Significant differences in chromosome size, ranging from 2AE4 to 4AE2 Mb for the B. thuringiensis strains studied, were noted. Strain heterogeneity in pulsotypes was also reflected by the similarity of whole-cell protein profiles of the strains. Environmental isolates and reference strains grouped at 71% similarity according to SDS-PAGE data and at 84% on the basis of biochemical tests. Conclusions: B. thuringiensis from intestines of bank voles demonstrated an important level of heterogeneity. The comparison of PFGE profiles and SDS-PAGE of whole-cell protein patterns may be useful to evaluate the relationship between B. thuringiensis isolates. Significance and Impact of the Study: The results presented in this paper may help to explain the diversity of B. thuringiensis.

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Characterization by Polyacrylamide Gel Electrophoresis of Whole-Cell Proteins of Some Bacillus thuringiensis subsp. israelensis Strains Isolated in Brazil

Cited by 12 publications

References 15 publications

Population Structure and Evolution of the Bacillus cereus Group

Population Structure and Evolution of the Bacillus cereus Group

Pulsed field gel electrophoresis of chromosomal DNA reveals a clonal population structure toBacillus thuringiensisthat relates in general to crystal protein gene content

Properties of Bacillus thuringiensis isolated from bank voles

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