Characterization and virulence of Beauveria spp. recovered from emerald ash borer in southwestern Ontario, Canada

Johny, Shajahan; Kyei-Poku, George; Gauthier, Debbie; Frankenhuyzen, Kees van; Krell, Peter J.

doi:10.1016/j.jip.2012.05.008

Cited by 26 publications

(17 citation statements)

References 35 publications

(60 reference statements)

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“…In Canada, the isolate B. bassiana INRS‐242 has also demonstrated its effectiveness in infecting other Coleopteran species, such as the emerald ash borer, Agrilus planipennis (Johny et al. ), the tarnished plant bug, Lygus lineolaris, the strawberry bud weevil, Anthonomus signatus , and the strawberry root weevil, Otiorhynchus ovatus (Sabbahi et al. ,b), the white pine weevil, Pissodes strobi (Trudel et al.…”

Section: Discussionmentioning

confidence: 99%

Susceptibility of Dendroctonus simplex to Hypocreales fungi: towards the development of a biological control strategy

Srei¹,

Lavallée

Guertin³

2016

J Applied Entomology

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The use of Hypocreales fungi against bark beetles is an attractive alternative strategy for biological control in forestry. However, fungal inoculation methods remain a major challenge due to the cryptic behaviour of these beetles. Experiments were conducted to evaluate the susceptibility of adult eastern larch beetles, Dendroctonus simplex, to different isolates of Hypocreales fungi and to assess the efficiency of direct inoculation of fungal conidia using an assisted autodissemination device. Among the nine fungal isolates tested, Beauveria bassiana INRS‐242 was selected as the utmost isolate causing a mortality rate of 64.4% of D. simplex adults within 10 days and having a high conidial viability of 81.7%. Using an assisted autodissemination device, a fungal‐coated pouch was a promising contamination chamber. Once it had fallen onto the fungal‐coated pouch, each D. simplex adult loaded an average lethal dose of 2.1 × 109 conidia, which induced a mortality rate of 98.0%. Our results demonstrated the fungal pathogenicity of B. bassiana INRS‐242 against the eastern larch beetles and pointed out the potential of the assisted autodissemination device as an effective method of conidia inoculation. These findings represent the first step to develop a biological control strategy against D. simplex populations.

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Section: Discussionmentioning

confidence: 99%

Susceptibility of Dendroctonus simplex to Hypocreales fungi: towards the development of a biological control strategy

Srei¹,

Lavallée

Guertin³

2016

J Applied Entomology

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“…Isolates of B. bassiana and M. anisopliae s.l. exhibit high genetic and virulence diversity [27, [36][37][38][39][40]. Comparative genomic analyses for both genera have revealed qualitative differences in gene content that are potentially important in determining host virulence [41][42][43][44][45].…”

Section: Insect Pathogensmentioning

confidence: 99%

Managing the plant microbiome for biocontrol fungi: examples from Hypocreales

Kepler

Maul

Rehner³

2017

Current Opinion in Microbiology

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“…However, conidia germination of the L49 Ð1AA isolate was poor, never exceeding 6%, indicating that the few conidia produced were not viable. Johny et al (2012) reported this isolated to be more virulent to emerald ash borer and produced more conidia on cadavers of emerald ash borer than did other indigenous isolates or the commercial GHA strain of B. bassiana. Poor germination in the simulated Þeld experiment may be because of storage of the pouches in the cold during active sporulation before deployment in the autocontamination traps.…”

Section: Discussionmentioning

confidence: 82%

“…1; Table 2). Earlier investigations (Johny et al 2012) demonstrated that the fungus is pathogenic to emerald ash borer. Visual examination of the cultures in the pouches revealed low conidia production.…”

Section: Discussionmentioning

confidence: 99%

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Towards the Development of an Autocontamination Trap System to Manage Populations of Emerald Ash Borer (Coleoptera: Buprestidae) With the Native Entomopathogenic Fungus, <I>Beauveria bassiana</I>

Lyons¹,

Iavallée²,

Kyei-Poku³

et al. 2012

jnl. econ. entom.

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Emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae) is an invasive species from Asia that was discovered in North America Canada, in 2002. Herein, we describe studies to develop an autocontamination trapping system to disseminate Beauveria bassiana to control beetle populations. The standard trap for emerald ash borer in Canada is a light green prism trap covered in an insect adhesive and baited with (Z)-3-hexenol. We compared of green multifunnel traps, green intercept panel traps (both with and without fluon coating) and green prism traps for capturing emerald ash borer in a green ash plantation. The coated green multifunnel traps captured significantly more males and more females than any other trap design. We examined the efficacy of two native B. bassiana isolates, INRS-CFL and L49-1AA. In a field experiment the INRS-CFL isolate attached to multifunnel traps in autocontamination chambers retained its pathogenicity to emerald ash borer adults for up to 43 d of outdoor exposure. Conidia germination of the INRS-CFL isolate was >69% after outdoor exposure in the traps for up to 57 d. The L49-1AA isolate was not pathogenic in simulated trap exposures and the germination rate was extremely low (<5.3%). Mean (+/- SEM) conidia loads on ash borer adults after being autocontaminated in the laboratory using pouches that had been exposed in traps out of doors for 29 d were 579,200 (+/- 86,181) and 2,400 (+/- 681) for the INRS-CFL and the LA9-1AA isolates, respectively. We also examined the fungal dissemination process under field conditions using the L49-1AA isolate in a green ash plantation. Beetles were lured to baited green multifunnel traps with attached autocontamination chambers. Beetles acquired fungal conidia from cultures growing on pouches in the chambers and were recaptured on Pestick-coated traps. In total, 2,532 beetles were captured of which 165 (6.5%) had fungal growth that resembled B. bassiana. Of these 25 beetles were positive for the L49-1AA isolate.

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Characterization and virulence of Beauveria spp. recovered from emerald ash borer in southwestern Ontario, Canada

Cited by 26 publications

References 35 publications

Susceptibility of Dendroctonus simplex to Hypocreales fungi: towards the development of a biological control strategy

Susceptibility of Dendroctonus simplex to Hypocreales fungi: towards the development of a biological control strategy

Managing the plant microbiome for biocontrol fungi: examples from Hypocreales

Towards the Development of an Autocontamination Trap System to Manage Populations of Emerald Ash Borer (Coleoptera: Buprestidae) With the Native Entomopathogenic Fungus, <I>Beauveria bassiana</I>

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