Characterization and utility of phages bearing peptides with affinity to porcine reproductive and respiratory syndrome virus nsp7 protein

Wang, Heng; Liu, Rongchang; Cui, Jin; Deng, Shulin; Xie, Jiexiong; Nin, Zhangyong; Zhang, Guihong

doi:10.1016/j.jviromet.2015.04.026

Cited by 7 publications

(5 citation statements)

References 27 publications

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“…Although it is common to screen in-house phage display libraries, the commercial peptide libraries (e.g., New England BioLabs (NEB) and MoBiTec GmbH) have also been used to develop peptide-based antivirals [50][51][52]. For instance, 7-mer and 12-mer linear peptide libraries of NEB have been widely used to identify antivirals for various viruses (e.g., avian infectious bronchitis virus (IBV), dengue virus serotype 2 (DENV-2), Macrobrachium rosenbergii nodavirus (MrNv), Japanese encephalitis virus (JEV), porcine reproductive and respiratory syndrome virus (PRRSV), porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis (TGE) virus, infectious salmon anemia virus (ISAV), bovine ephemeral fever virus (BEFV), influenza B virus, and Mink enteritis virus (MEV)) (Table 1) [53][54][55][56][57][58][59][60][61][62][63][64]. Cao et al [65] employed a 12-mer phage display peptide library to develop antivirals against PEDV, the causative agent of persistent diarrhea in swine.…”

Section: Peptide Librariesmentioning

confidence: 99%

Discovery of Antivirals Using Phage Display

Sokullu

Gauthier

Coulombe

2021

Viruses

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The latest coronavirus disease outbreak, COVID-19, has brought attention to viral infections which have posed serious health threats to humankind throughout history. The rapid global spread of COVID-19 is attributed to the increased human mobility of today’s world, yet the threat of viral infections to global public health is expected to increase continuously in part due to increasing human–animal interface. Development of antiviral agents is crucial to combat both existing and novel viral infections. Recently, there is a growing interest in peptide/protein-based drug molecules. Antibodies are becoming especially predominant in the drug market. Indeed, in a remarkably short period, four antibody therapeutics were authorized for emergency use in COVID-19 treatment in the US, Russia, and India as of November 2020. Phage display has been one of the most widely used screening methods for peptide/antibody drug discovery. Several phage display-derived biologics are already in the market, and the expiration of intellectual property rights of phage-display antibody discovery platforms suggests an increment in antibody drugs in the near future. This review summarizes the most common phage display libraries used in antiviral discovery, highlights the approaches employed to enhance the antiviral potency of selected peptides/antibody fragments, and finally provides a discussion about the present status of the developed antivirals in clinic.

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Section: Peptide Librariesmentioning

confidence: 99%

Discovery of Antivirals Using Phage Display

Sokullu

Gauthier

Coulombe

2021

Viruses

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“…The phage display technique has been used by several authors to search for interacting peptides, with the ultimate objective of developing new drugs against several viruses. Some examples include peptides described with activity against HIV [ 21 , 46 ], Puumala virus [ 47 ], West Nile virus [ 48 ], Newcastle virus [ 49 , 50 ], hepatitis C virus [ 51 , 52 ], porcine reproductive and respiratory syndrome virus [ 53 , 54 ], infectious bronchitis virus [ 55 ], hepatitis B virus [ 56 ], Mink enteritis virus [ 57 ], Japanese encephalitis virus [ 58 ], classical swine fever virus [ 59 ], and influenza virus [ 60 ].…”

Section: Resultsmentioning

confidence: 99%

Phage-Displayed Peptides Selected to Bind Envelope Glycoprotein Show Antiviral Activity against Dengue Virus Serotype 2

Guardia

Quijada

Lleonart

2017

Advances in Virology

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Dengue virus is a growing public health threat that affects hundreds of million peoples every year and leave huge economic and social damage. The virus is transmitted by mosquitoes and the incidence of the disease is increasing, among other causes, due to the geographical expansion of the vector's range and the lack of effectiveness in public health interventions in most prevalent countries. So far, no highly effective vaccine or antiviral has been developed for this virus. Here we employed phage display technology to identify peptides able to block the DENV2. A random peptide library presented in M13 phages was screened with recombinant dengue envelope and its fragment domain III. After four rounds of panning, several binding peptides were identified, synthesized, and tested against the virus. Three peptides were able to block the infectivity of the virus while not being toxic to the target cells. Blind docking simulations were done to investigate the possible mode of binding, showing that all peptides appear to bind domain III of the protein and may be mostly stabilized by hydrophobic interactions. These results are relevant to the development of novel therapeutics against this important virus.

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“…The purified ORF2 mAbs were applied as targets and subjected to biopanning using a Ph.D.-12 Phage Display Peptide Library Kit (New England Biolabs, MA, USA). The procedure was performed according to the manufacturer’s instructions and a previous study [ 13 ]. The ratio in each round was calculated to analyze the enrichment efficiency using the titer of the amplified phages in the input buffer (input value) and in the elution buffer (output value).…”

Section: Methodsmentioning

confidence: 99%

“…The candidate peptides from the phage display biopanning were synthesized by ChinaPeptides Co., Ltd. (Shanghai, China), according to the standard used in our previous study [ 13 ]. The affinity of the synthetic peptides to ORF2 mAbs was detected by ELISA.…”

Section: Methodsmentioning

confidence: 99%

Identification and characterization of two linear epitope motifs in hepatitis E virus ORF2 protein

Wang

Zhang

et al. 2017

PLoS ONE

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Hepatitis E virus (HEV) is responsible for hepatitis E, which represents a global public health problem. HEV genotypes 3 and 4 are reported to be zoonotic, and animals are monitored for HEV infection in the interests of public hygiene and food safety. The development of novel diagnostic methods and vaccines for HEV in humans is thus important topics of research. Opening reading frame (ORF) 2 of HEV includes both linear and conformational epitopes and is regarded as the primary candidate for vaccines and diagnostic tests. We investigated the precise location of the HEV epitopes in the ORF2 protein. We prepared four monoclonal antibodies (mAbs) against genotype 4 ORF2 protein and identified two linear epitopes, G438IVIPHD444 and Y457DNQH461, corresponding to two of these mAbs using phage display biopanning technology. Both these epitopes were speculated to be universal to genotypes 1, 2, 3, 4, and avian HEVs. We also used two 12-mer fragments of ORF2 protein including these two epitopes to develop a peptide-based enzyme-linked immunosorbent assay (ELISA) to detect HEV in serum. This assay demonstrated good specificity but low sensitivity compared with the commercial method, indicating that these two epitopes could serve as potential candidate targets for diagnosis. Overall, these results further our understanding of the epitope distribution of HEV ORF2, and provide important information for the development of peptide-based immunodiagnostic tests to detect HEV in serum.

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Characterization and utility of phages bearing peptides with affinity to porcine reproductive and respiratory syndrome virus nsp7 protein

Cited by 7 publications

References 27 publications

Discovery of Antivirals Using Phage Display

Discovery of Antivirals Using Phage Display

Phage-Displayed Peptides Selected to Bind Envelope Glycoprotein Show Antiviral Activity against Dengue Virus Serotype 2

Identification and characterization of two linear epitope motifs in hepatitis E virus ORF2 protein

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