Characterization and use of equine bone marrow mesenchymal stem cells in horse cartilage engineering

Abstract

results indicate that the amyloid structures could potentially be used for cartilage tissue engineering. The next steps include culturing chondrocytes with amyloid structures in a 3D hydrogel to provide a more realistic surrounding to the cells and to preserve the chondrocyte phenotype. Microscopy image of bovine chondrocytes (dark) in culture with a-synuclein amyloid aggregates (transparent). The dark color of the cells is caused by formazan crystals as a result of the MTT assay. Scale bar represent 100 mm.

Deciphering the transcriptional activities of genes coding for adipokines and their receptors in porcine ex situ-protected mesenchymal stem cells undergoing adipogenic differentiation

“…After the stable MSC lines had been propagated and multiplied, the multipotencyrelated attributes of their random representatives were identified by the ectopic onset of chondrogenic differentiability. The processes of the functional reprogramming of MSCs into chondroblasts/chondrocytes took place in low-glucose-formulated DMEM enriched with 15% FBS (Sigma-Aldrich, Merck, Poznań, Poland), 1× Glutamax (Invitrogen, Thermofisher Scientific, Waltham, Massachusetts, USA), 1% antibiotic-antimycotic solution (AAS; Sigma-Aldrich, Merck, Poznań, Poland), and the cocktail of supplements triggering cytodifferentiation as elaborated by Branly et al (2017). This cocktail comprised 100 nM dexamethasone (Sigma-Aldrich, Merck, Poznań, Poland), 50 μg/mL L-ascorbic acid 2-phosphate (Sigma-Aldrich, Merck, Poznań, Poland), 40 μg/mL L-proline (Sigma-Aldrich, Merck, Poznań, Poland), 1 nM sodium pyruvate (Sigma-Aldrich, Merck, Poznań, Poland), 1% insulin-transferrin-sodium selenite (ITS) solution (Sigma-Aldrich, Merck, Poznań, Poland), and 10 ng/mL transforming growth factor-β1 (TGF-β1; Sigma-Aldrich, Merck, Poznań, Poland).…”