Characterization and transgenic study of CONSTANS-LIKE8 (COL8) gene in Arabidopsis thaliana: expression of 35S:COL8 delays flowering under long-day conditions

Takase, Tomoyuki; Kakikubo, Yoshihiro; Nakasone, Akari; Nishiyama, Yuka; Yasuhara, Masahiro; Tokioka-Ono, Yoko; Kiyosue, Tomohiro

doi:10.5511/plantbiotechnology.11.0823b

Cited by 40 publications

(23 citation statements)

References 42 publications

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“…Previous studies have demonstrated that a functional B-box II domain is required for flower induction (Griffiths et al, 2003;Martin et al, 2004;Campoli et al, 2012). Interestingly, the overexpression of COL8, which is a COL TF with a single B-box domain, resulted in a phenotype with delayed flowering under LD conditions in Arabidopsis (Takase et al, 2011). Therefore, it seems that the absence of the B-box II domain in some COL TFs does not necessarily affect the regulation of flowering.…”

Section: Identification Of Six Bncol Tfs With a Full-length Orfmentioning

confidence: 91%

“…Seventeen members, including CO genes, were reported in Arabidopsis , whereas 16 COL TFs and 9 COL TFs were identified in rice and barley, respectively (Griffiths et al, 2003). Some COL members that were identified from Arabidopsis, rice, barley, and ryegrass have been characterized by their functions; the results revealed that most COL TFs either promoted or delayed flowering under LD or SD conditions (Yano et al, 2000;Suarez-Lopez et al, 2001;Martin et al, 2004;Cheng and Wang, 2005;Kim et al, 2008;Xue et al, 2008;Hassidim et al, 2009;Lee et al, 2010;Takase et al, 2011;Campoli et al, 2012;Kikuchi et al, 2012;Wu et al, 2013). These findings suggest that many COL family members have a conserved function in modulating flowering via the photoperiodic response.…”

Section: Introductionmentioning

confidence: 92%

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Identification of a CONSTANS homologous gene with distinct diurnal expression patterns in varied photoperiods in ramie (Boehmeria nivea L. Gaud)

Liu

Zhu

Tang

et al. 2015

Gene

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Section: Identification Of Six Bncol Tfs With a Full-length Orfmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 92%

Identification of a CONSTANS homologous gene with distinct diurnal expression patterns in varied photoperiods in ramie (Boehmeria nivea L. Gaud)

Liu

Zhu

Tang

et al. 2015

Gene

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“…The COL gene functions as a transcription factor in multiple growth and development pathways, and particularly in the photoperiod-mediated owering pathway. Some genes in this family have been found to play an important role in the light response-mediated regulation of owering [5,[17][18][19], with functions that differ between short-day (SD) and long-day (LD) conditions. For example, OsCOL10, OsCOL13 and OsCOL16 function as negative regulators of owering under both SD and LD condition in rice, while Hd1, a member of the COL gene family, promotes owering under SD and suppresses owering under LD [5,17,[20][21].…”

Section: Introductionmentioning

confidence: 99%

“…For example, OsCOL10, OsCOL13 and OsCOL16 function as negative regulators of owering under both SD and LD condition in rice, while Hd1, a member of the COL gene family, promotes owering under SD and suppresses owering under LD [5,17,[20][21]. In Arabidopsis, the overexpression of AtCOL3, AtCOL7 and AtCOL8 can delay owering time, while, in the contrast, the overexpression of the AtCOL5 gene promotes owering by enhancing the expression of FLOWERING LOCUS T (FT) [18][19][22][23]. Similar to their functions, the expression patterns also vary among the members of the COL gene family.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide Identification, Expression, and Sequence Analysis of CONSTANT-like Gene Family in Cannabis Reveals Role in Plant Flowering Time Regulation

Pan

Yin

et al. 2020

Preprint

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Background: Cannabis, an important industrial crop, has a high sensitivity to photoperiods. The flowering time of cannabis is one of its important agronomic traits, and has an important effect on its yield and quality. The CONSTANS-like (COL) gene plays a key role in the regulation of flowering in this plant. However, the specific biological and functional roles of the COL gene family in the cannabis is still unknown. Results: In this study, 13 CsCOL genes were identified in the cannabis genome. Phylogenetic analysis implied that the CsCOL proteins were divided into three subgroups, and each subgroups included conserved intron/exon structures and motifs. Chromosome distribution analysis showed that 13 CsCOL genes were unevenly distributed on 7 chromosomes, with chromosome 10 having the most CsCOL members. Colinearity analysis showed that two syntenic gene pairs of CsCOL4 and CsCOL11 were found in both rice and Gossypium raimondii. Among 13 CsCOLs, CsCOL6 and CsCOL12 were a pair of tandem duplicated genes, whereas CsCOL8 and CsCOL11 may have resulted from segmental duplication. Furthermore, tissue-specific expression showed that ten CsCOL genes were preferentially expressed in the leaves, one CsCOL in the stem, and two CsCOL in the female flower. Most CsCOL exhibited a diurnal oscillation pattern under different light treatment. Additionally, sequence analysis showed that CsCOL3 and CsCOL7 exhibited amino acid difference among the early-flowering cultivars and late flowering cultivars. Conclusion: This study provided insight into the potential functions studies of CsCOL genes, and highlight its roles in the regulation of flowering time in cannabis. Our results laid a foundation for the further elucidation of the functions of COLs in cannabis

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“…Among them, COL1 and COL2 have little effect on the flowering time, while COL3, COL4, and COL9 represent flowering repressors [27][28][29] . The overexpression of COL8 delays flowering under LDs, whereas COL5 promotes flowering 30,31 . Multiple CO/COL homologues have been identified in different species: 16 in rice, 9 in barley, 13 in sugar beet, and 28 in soybean [32][33][34][35] .…”

mentioning

confidence: 99%

The Response of COL and FT Homologues to Photoperiodic Regulation in Carrot (Daucus carota L.)

Liu

Chen

et al. 2020

Sci Rep

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carrot (Daucus carota L.) is a biennial plant requiring vernalization to induce flowering, but long days can promote its premature bolting and flowering. The basic genetic network controlling the flowering time has been constructed for carrot, but there is limited information on the molecular mechanisms underlying the photoperiodic flowering response. The published carrot genome could provide an effective tool for systematically retrieving the key integrator genes of GIGANTEA (GI), CONSTANS-LIKE (COL), FLOWERING LOCUS T (FT), and SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1) homologues in the photoperiod pathway. In this study, the bolting time of wild species "Songzi" (Ws) could be regulated by different photoperiods, but the orange cultivar "Amsterdam forcing" (Af) displayed no bolting phenomenon. According to the carrot genome and previous de novo transcriptome, 1 DcGI, 15 DcCOLs, 2 DcFTs, and 3 DcSOC1s were identified in the photoperiod pathway. The circadian rhythm peaks of DcGI, DcCOL2, DcCOL5a, and DcCOL13b could be delayed under long days (LDs). The peak value of DcCOL2 in Af (12.9) was significantly higher than that in Ws (6.8) under short day (SD) conditions, and was reduced under LD conditions (5.0). The peak values of DcCOL5a in Ws were constantly higher than those in Af under the photoperiod treatments. The expression levels of DcFT1 in Ws (463.0) were significantly upregulated under LD conditions compared with those in Af (1.4). These responses of DcCOL2, DcCOL5a, and DcFT1 might be related to the different bolting responses of Ws and Af. This study could provide valuable insights into understanding the key integrator genes in the carrot photoperiod pathway.

show abstract

Characterization and transgenic study of CONSTANS-LIKE8 (COL8) gene in Arabidopsis thaliana: expression of 35S:COL8 delays flowering under long-day conditions

Cited by 40 publications

References 42 publications

Identification of a CONSTANS homologous gene with distinct diurnal expression patterns in varied photoperiods in ramie (Boehmeria nivea L. Gaud)

Identification of a CONSTANS homologous gene with distinct diurnal expression patterns in varied photoperiods in ramie (Boehmeria nivea L. Gaud)

Genome-wide Identification, Expression, and Sequence Analysis of CONSTANT-like Gene Family in Cannabis Reveals Role in Plant Flowering Time Regulation

The Response of COL and FT Homologues to Photoperiodic Regulation in Carrot (Daucus carota L.)

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