Characterization and the evaluation of antimicrobial activities of silver nanoparticles biosynthesized from Carya illinoinensis leaf extract

Abstract

A green, direct and cost-effective fabrication method is proposed for Eco-environmentally silver nanoparticles (AgNPs) through leaf extraction of Carya illinoinensis from Iran. Formation of Ag NPs was confirmed through different characterization techniques such as UV-Vis Spectroscopy, X-ray Diffraction (XRD), Scanning Electron Microscope (SEM) and Transmission Electron Microscopy (TEM). UV-Visible spectrophotometer showed absorbance peak at 440 nm due to the Surface Plasmon Resonance (SPR). Based on XRD results and SEM and TEM analysis, AgNPs were crystalline with face-centered cubic geometry and in different sizes ranged 12-30 nm. Furthermore, FTIR Spectroscopy was utilized to recognize the specific functional groups responsible for reducing ion silver to silver nanoparticles and the capping agents available in the leaf extract. In addition, the antibacterial effect of Eco-friendly synthesized nanoparticles and also leaf extract, were evaluated on four pathogens by implementing minimum inhibitory concentration test (MIC) and agar diffusion assay. The MIC results exhibits more inhibiting activity against gram-negative microorganisms (Escherichia coli and Pseudomonas aeruginosa) rather than gram-positive microorganisms (Staphylococcus aureus and Listeria monocytogenes). Compared to leaf extract, nanoparticles have better antimicrobial activity against both Gram-positive and Gram-negative bacteria.

Silver nanoparticles from insect wing extract: Biosynthesis and evaluation for antioxidant and antimicrobial potential

“…sp . lycopersici MTCC 10270, Phytophthora nicotianae , Fusarium sacchari and Colletotrichum falcatum using agar well diffusion method [ 15 , 16 ] using nutrient and potato dextrose agar plates (Fungal strains were obtained from Indian Institute of Oil Seeds Research (IIOR) Rajendranagar,, Hyderabad, TS, India and Regional Agricultural Research Station (RARS), Anakapalle, AP, India. Aliquots of 50 μL of different concentrations of MMAgNPs (10 μg mL -1 , 5 μg/mL, 2.5 μg mL -1 and 1.25 μg mL -1 ) were separately added in the wells.…”

Bioavailability and antioxidant activity of nanotechnology‐based botanic antioxidants

“…The minimum inhibitory concentration (MIC) value for AgNPs inhibited on the testing bacteria was observed in the concentration range of 4 to 16 µg/mL (Keshari et al., 2020). Treatment with AgNPs with plant extract presented higher antibacterial activity on gram negative bacteria than positive one and fungus (Aguilar et al., 2018; Baruah, Yadav, Yadav, & Das, 2019; Javan bakht Dalir, Djahaniani, Nabati, & Hekmati, 2020), while AgNPs by using Hibiscus Rosa–Sinensis extract demonstrated better antibacterial activity against Staphylococcus aureus than E. coli (A. Singh et al., 2020). Besides, AuNPs exhibited the antimicrobial activity against E. coli , Bacillus subtilis , and Candida albicans with the MIC of 25, 50, and 32 µg/mL, respectively (Ahmad et al., 2017; Dananjaya et al., 2020).…”

“…This observation may be due to the higher surface activity of the AgNPs as compared to the AuNPs and higher penetrating power. The smaller size of AgNPs provides improved exposure to the microorganism (Javan bakht Dalir et al., 2020; Onitsuka, Hamada, & Okamura, 2019). In addition, this may be a reminder to devolop smaller AuNPs on the purpose of antimicrobial agents.…”

Fabrication of Dexamethasone-Silver Nanoparticles Entrapped Dendrimer Collagen Matrix Nanoparticles for Dental Applications

“…To understand the minimum inhibitory effect of synthesized and fabricated nanomaterial [45,46], AgNP@Den@Dex@Col was carried out in different concentrations ranging from 5 to 35 µg/mL in well and incubated for 24 hours. To understand the effect of silver nanoparticles alone, dendrimer alone, silver nanoparticles functionalized with dendrimer and entrapped with dexamethasone and cross-linked dendrimers were studied for the inhibitory effect of these against gram-positive bacteria (Staphylococcus aureus) and gram-negative bacteria (Klebsiella pneumonia).…”