Characterization and Synthesis of STP-3

Yuen, Sylvia; Otteson, K M; Colburn, Joel C.; Moore, William T.; Schlabach, Tim D.; Dupont, David; Mattaliano, Robert J.

doi:10.1016/b978-0-12-682001-0.50068-3

Techniques in Protein Chemistry 1989

DOI: 10.1016/b978-0-12-682001-0.50068-3

|View full text |Cite

Characterization and Synthesis of STP-3

Sylvia Yuen¹,

K M Otteson²,

Joel C. Colburn³

et al.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

1989

1996

Publication Types

Select...

Article2

Relationship

Self Cite1

Independent1

Authors

Journals

Cited by 2 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Separation of five endorphin species by FSCE. The five species are /3-endorphin (1-31), Des-Tyr-/3-endorphin (2-31), Nacetyl-/3-endorphin (N-Ac-1-31), 7-endorphin (1-17), and Des-Tyr-7endorphin(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17). Multiple peaks in sample 2-17 are presumed to be caused by unknown contaminants.…”

mentioning

confidence: 99%

Application of free-solution capillary electrophoresis to the analytical scale separation of proteins and peptides

Grossman¹,

Colburn²,

Lauer³

et al. 1989

Anal. Chem.

Self Cite

277

View full text Add to dashboard Cite

The application of free solution capillary electrophoresis (FSCE) to the separation of protein and peptide mixtures is presented. Both qualitative and quantitative aspects of FSCE separations are considered. In addition, a brief introduction describing the separation principle behind FSCE separations and a discussion of electrophoretic mobility are included. The applications were chosen in order to highlight the selectivity of FSCE separations and to demonstrate applications of potential practical interest to the bioanalytical chemist. Comparison of FSCE relative to traditional analytical separation alternatives is stressed throughout. The examples are presented in three broad categories: protein separations, peptide separations, and the application of both to the analysis of recombinant protein products. In the first section, FSCE separations of peptide mixtures are presented which demonstrate the suitability of FSCE for the analysis of the purity of peptide samples, the homogeneity of peptide samples prior to sequencing, the identity of peptides by using electrophoretic mobility values, and the reduction of an intrachain disulfide bridge. In the second section, protein separations are presented that show the resolution of glycoproteins having the same primary structure and the separation of immune complexes from free unreacted antibody and antigen. In the final section, highly purified and well-characterized samples of biosynthetic human insulin (BHI), biosynthetic human growth hormone (hGH), and their derivatives were used to evaluate FSCE as a complement and/or alternative to conventional analytical separation techniques for the determination of purity and identity of biosynthetic human proteins. In addition, the quantitative aspects of FSCE analysis such as linearity of response, precision, and limit of detection were examined.

show abstract

mentioning

confidence: 99%

Application of free-solution capillary electrophoresis to the analytical scale separation of proteins and peptides

Grossman¹,

Colburn²,

Lauer³

et al. 1989

Anal. Chem.

Self Cite

277

View full text Add to dashboard Cite

show abstract

Synthesis, structure and stability of novel dimeric peptide‐disulfides

Leban

Spaltenstein

Landavazo

et al. 1996

International Journal of Peptide and Protein Research

View full text Add to dashboard Cite

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Characterization and Synthesis of STP-3

Cited by 2 publications

References 0 publications

Application of free-solution capillary electrophoresis to the analytical scale separation of proteins and peptides

Application of free-solution capillary electrophoresis to the analytical scale separation of proteins and peptides

Synthesis, structure and stability of novel dimeric peptide‐disulfides

Contact Info

Product

Resources

About