Characterization and survival of broad-spectrum biocontrol agents against phytopathogenic fungi

Azeem, Saba; AGHA, Syed I.; Jamil, Neelam; Tabassum, Bushra; Ahmed, Shan; Raheem, Asif; Jahan, Nusrat; Ali, Niaz; Khan, Anwar

doi:10.1016/j.ram.2021.10.005

Cited by 9 publications

(5 citation statements)

References 24 publications

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“…The production of HCN by PGPRs played an important role in the biological control of several soil pathogenic fungi, as reported by Azeem al. [64]. And as our results show (Table 1), these bacteria produce, to different degrees, these bioactive molecules which inhibit the growth of fungi.…”

Section: Discussionmentioning

confidence: 70%

Halophilic Plant Growth Promoting Rhizobacteria as Producers of Antifungal Metabolites under Salt Stress

Ould Ouali,

Houali,

Cruz

et al. 2024

Preprint

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Salinity is one of the major factors responsible for the deterioration of soils, making them unsuitable for agriculture. The application of halotolerant and halophilic plant growth promoting bacteria (PGPR: Plant Growth Promoting Rhizobacteria) with biological control activities as an inoculant of cultivated plants offers a biological alternative to the use of agrochemicals, particularly when subjected to salt stress. In this perspective, 70 bacterial strains were isolated from saline soils (sebkha) in arid and semi-arid areas of eastern Algeria. Three isolates were selected based on their ability to produce bioactive molecules allowing them to promote plant growth, such as hydrolytic enzymes, indole acetic acid, HCN, NH3, etc. Two of these isolates belong to the genus Serratia and the third is a halophilic Halomonas bacteria. These bacteria were identified based on the 16S rDNA sequence. Antagonism tests against phytopathogenic fungi were carried out. The identification of the antifungal molecules produced by these bacteria was done by high performance liquid chromatography. These bacteria are capable of inhibiting mycelial development against phytopathogenic fungi with rates reaching 80.67% against Botrytis cinerea, 76.22% against Aspergillus niger and 66.67% against Fusarium culmorum for Serratia sp. The strain Halomonas sp. inhibited mycelial growth by production of volatile substances of Aspergillus niger at 71.29%, Aspergillus flavus at 75.49% and Penicillium glabrum at a rate of 72.22%. The identification by HPLC of the antifungal molecules produced by these three bacteria reveals that they are polyphenols, which makes these strains the first rhizobacteria capable of producing phenolic compounds. Finally, pot tests to determine the effectiveness of these strains in promoting wheat growth under salinity stress (125 mM, 150 mM and 200 mM) were carried out. The results revealed that a consortium of two isolates (Serratia sp. and Halomonas sp.) performed best at 125 mM. But, at higher concentrations it was the halophilic bacteria Halomonas sp which gave the best result. In all cases, there was a significant improvement in the growth of wheat seedlings inoculated with the bacteria, compared to non-inoculated controls.

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Section: Discussionmentioning

confidence: 70%

Halophilic Plant Growth Promoting Rhizobacteria as Producers of Antifungal Metabolites under Salt Stress

Ould Ouali,

Houali,

Cruz

et al. 2024

Preprint

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“…Specifically, P. protegens produces pyoverdine as iron-scavenging compound ( Li et al., 2022 ). HCN is a toxic molecule to all aerobic organisms, including fungi since it can block cytochrome c oxidase and other metalloenzymes ( Azeem et al., 2022 ). Multiple Pseudomonas species, such as P. putida R32, P. chlororaphis R47, and P. chlororaphis PA23 have been reported to be able to produce HCN and that contributed to the reduction of mycelia growth of pathogenic fungi ( Nandi et al., 2017 ; Anand et al., 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Biocontrol potential of Pseudomonas protegens ML15 against Botrytis cinerea causing gray mold on postharvest tomato (Solanum lycopersicum var. cerasiforme)

Ajijah,

Fiodor,

Dziurzynski

et al. 2023

Front. Plant Sci.

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Gray mold, caused by Botrytis cinerea is a major cause of post-harvest rot of fresh fruits and vegetables. The utilization of selected microorganisms as biocontrol agents is a promising alternative to effectively control gray mold on tomatoes. The current study was conducted to explore potential biocontrol mechanisms of the Pseudomonas strain to control infections on post-harvest tomatoes. Among the 8 tested bacterial isolates, Pseudomonas protegens ML15 demonstrated antagonistic activity to Botrytis cinerea. Moreover, P. protegens ML15 exhibited the production of siderophores, hydrogen cyanide, ammonia, exopolysaccharides, lipase, biosurfactant, 2,4-diacetylphloroglucinol, and several other antifungal compounds, such as 1-tetradecanol, cyclododecane, 2,4-di-tert-butylphenol, and 2-methyl-1-hexadecanol. A comprehensive genomic analysis of P. protegens ML15 unravels 18 distinct genetic regions with the potential for biosynthesizing secondary metabolites, known for their pivotal role in biocontrol responses against plant pathogens. In vivo, experiments showed that both culture suspension and cell-free supernatant of P. protegens ML15 significantly reduced fungal growth (53.0 ± 0.63%) and mitigated disease development (52.8 ± 1.5%) in cherry tomatoes at four days post-B. cinerea inoculation. During the infection, the application of P. protegens ML15 resulted in the augmentation of total antioxidant, phenolic content, and ascorbic acids content. Thus, our results suggested that P. protegens ML15’s role as a biocontrol agent against B. cinerea-induced postharvest tomato decay achieved through the secretion of antifungal substances, induction of tomato defense responses, and inhibition of mycelial growth of B. cinerea. These findings provide a significant contribution to the ongoing search for alternative, eco-friendly methods of controlling gray mold in fresh products. The utilization of P. protegens ML15 as a biocontrol agent could help to reduce the reliance on chemical fungicides and promote sustainable agriculture practices.

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“…BCB16, the chosen isolate, was stored in talc powder for 4 months to test its survival. Talc powder was used for survival because it is a low-cost carrier material that can be used in commercial biofungicide production [ 2 ]. Talc powder was autoclaved for 15 min at 121°C and 15 psi to remove any contamination.…”

Section: Methodsmentioning

confidence: 99%

“…Phytopathogens are threatening agricultural resources, with fungal pathogens being the most serious [ 2 ]. Pathogenic fungi play a role in the global loss of agricultural yield.…”

Section: Introductionmentioning

confidence: 99%

Biocontrol rhizobacteria enhances growth and yield of wheat ( Triticum aestivum ) under field conditions against Fusarium oxysporum

Agha,

Ullah,

Khan

et al. 2023

Bioengineered

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The current study aimed to identify the survival of bio-control bacteria with antifungal activity against Fusarium oxysporum and assess their growth promoting activity in wheat crop field conditions. To evaluate the fungicidal activities of isolated bacteria using the dual culture method, both qualitative and quantitative bioassays were performed. Plant Growth Promoting activities such as Indole 3-Acetic Acid (IAA), phosphate solubilization, Hydrogen cyanide (HCN), and Siderophore production were assessed for three biocontrol bacterial isolates (BCB 07, BCB16, and BCB 83) out of 180 with 70% antagonistic activity against Fusarium oxysporum . Chitinase, protease, and cellulase interaction in isolates was also tested. BCB16 was selected as it had 70% antagonist activity against F. oxysporum but also had the highest PGPR (Plant Growth Promoting Rhizobacteria) traits when compared to the other two isolates. BCB16 was also tested for survival in talc powder and in wheat crop field conditions. Even after 4 months in talc powder, the survival rate remained stable. In a wheat crop field, BCB16 reduced the disease incidence of Fusarium oxysporum by 54.38%. When compared to fungus alone treatment, BCB16 increased average yield by 57% alone and 32% in challenged conditions. BCB16 was identified molecularly using the 16s rRNA gene. Bacillus amyloliquefaciens shared 97% of the deduced sequence. The sequence was submitted to genbank and assigned the accession number OM333889. Bacillus amyloliquefaciens has the potential to be used in the field as an alternative to synthetic fungicides against Fusarium oxysporum .

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Characterization and survival of broad-spectrum biocontrol agents against phytopathogenic fungi

Cited by 9 publications

References 24 publications

Halophilic Plant Growth Promoting Rhizobacteria as Producers of Antifungal Metabolites under Salt Stress

Halophilic Plant Growth Promoting Rhizobacteria as Producers of Antifungal Metabolites under Salt Stress

Biocontrol potential of Pseudomonas protegens ML15 against Botrytis cinerea causing gray mold on postharvest tomato (Solanum lycopersicum var. cerasiforme)

Biocontrol rhizobacteria enhances growth and yield of wheat ( Triticum aestivum ) under field conditions against Fusarium oxysporum

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