Characterization and Structure Analysis of a Novel Bacteriocin, Lacticin Z, Produced by Lactococcus lactis QU 14

Journal of Applied Microbiology

Naruhiko

et al. 2011

Aims: To identify and characterize novel bacteriocins from Weissella hellenica QU 13. Methods and Results: Weissella hellenica QU 13, isolated from a barrel used to make Japanese pickles, produced two novel bacteriocins termed weissellicin Y and weissellicin M. The primary structures of weissellicins Y and M were determined, and their molecular masses were determined to be 4925·12 and 4968·40 Da, respectively. Analysis of the DNA sequence encoding the bacteriocins revealed that they were synthesized and secreted without N‐terminal extensions such as leader sequences or sec signal peptides. Weissellicin M showed significantly high and characteristic homology with enterocins L50A and L50B, produced by Enterococcus faecium L50, while weissellicin Y showed no homology with any other known bacteriocins. Both bacteriocins showed broad antimicrobial spectra, with especially high antimicrobial activity against species, which contaminate pickles, such as Bacillus coagulans, and weissellicin M showed relatively higher activity than weissellicin Y. Furthermore, the stability of weissellicin M against pH and heat was distinctively higher than that of weissellicin Y. Conclusions: Weissella hellenica QU 13 produced two novel leaderless bacteriocins, weissellicin Y and weissellicin M, and weissellicin M exhibited remarkable potency that could be employed by pickle‐producing industry. Significance and Impact of the Study: This study is the first report, which represents a complete identification and characterization of novel leaderless bacteriocins from Weissella genus.

Section: Discussionmentioning

confidence: 99%

Characterization and identification of weissellicin Y and weissellicin M, novel bacteriocins produced by Weissella hellenica QU 13

Masuda

Journal of Applied Microbiology

Naruhiko

et al. 2011

“…The bacteriocin was purified from the culture supernatant by a three-step procedure consisting of adsorption on Amberlite XAD-16 hydrophobic synthetic resin (Sigma, St. Louis, MO), SP-Sepharose cation exchange chromatography (GE Healthcare, Uppsala, Sweden), and reverse phase HPLC using a Resource RPC column (GE Healthcare), as described previously. 13) We obtained one active fraction at each purification step, indicating that S. hominis KQU-131 produces one kind of bacteriocin. The amino acid sequence of the bacteriocin was determined by Edman degradation with a gas-phase automatic sequence analyzer (PPSQ-21, Shimadzu, Kyoto, Japan).…”

mentioning

confidence: 84%

Identification of the Nukacin KQU-131, a New Type-A(II) Lantibiotic Produced byStaphylococcus hominisKQU-131 Isolated from Thai Fermented Fish Product (Pla-ra)

Wilaipun

Bioscience, Biotechnology, and Biochemistry

Okuda

et al. 2008

Self Cite

Staphylococcus hominis KQU-131, isolated from Thai fermented marine fish, produces a heat stable bacteriocin. Structural and genetic analysis indicated that the bacteriocin is a variant of nukacin ISK-1, a type-A(II) lantibiotic, and we termed the bacteriocin nukacin KQU-131. There were three different amino acid residues between nukacin ISK-1 and nukacin KQU-131, one residue in the leader peptide and the other two in the mature peptide.

“…In addition, another class-IId leaderless bacteriocin, lacticin Z, a homolog of lacticin Q, was identified from L. lactis QU 14, isolated from the horse intestine. 20) Lacticin Z was also characterized for structure, activity, and biosynthetic mechanism.…”

Section: Structural Determination and Characterization Of Novel Lmentioning

confidence: 99%

“…3) and in activity. 20) The mechanism of action of lacticin Q was studied comprehensively and compared with nisin A, which was also characterized in detail as to the action mechanism. Both lacticin Q and nisin A induced ATP leakage from target cells, but the action of lacticin Q was more rapid than that of nisin A.…”

Section: Structural Determination and Characterization Of Novel Lmentioning

confidence: 99%

Screening and Characterization of Novel Bacteriocins from Lactic Acid Bacteria

Bioscience, Biotechnology, and Biochemistry

2013

Self Cite

Bacteriocins produced by lactic acid bacteria (LAB) are expected to be safe antimicrobial agents. While the best studied LAB bacteriocin, nisin A, is widely utilized as a food preservative, various novel ones are required to control undesirable bacteria more effectively. To discover novel bacteriocins at the early step of the screening process, we developed a rapid screening system that evaluates bacteriocins produced by newly isolated LAB based on their antibacterial spectra and molecular masses. By means of this system, various novel bacteriocins were identified, including a nisin variant, nisin Q, a two-peptide bacteriocin, lactococcin Q, a leaderless bacteriocin, lacticin Q, and a circular bacteriocin, lactocyclicin Q. Moreover, some LAB isolates were found to produce multiple bacteriocins. They were characterized as to their structures, mechanisms of action, and biosynthetic mechanisms. Novel LAB bacteriocins and their biosynthetic mechanisms are expected for applications such as food preservation and peptide engineering.