Characterization and spinal fusion effect of rabbit mesenchymal stem cells

Lee, Tsung‐Han; Huang, Yuhua; Chang, Nyuk-Kong; Lin, Wei-Chiang; Chien, Pei-Wen Chang; Su, Tsung-Ming; Hsieh, Dar-Jen; Lee, Tao-Chen

doi:10.1186/1756-0500-6-528

Cited by 20 publications

(25 citation statements)

References 22 publications

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“…Alternatively, this could be due to antibody clone variation, and their differing affinity against markers. Taking into account that reports suggesting rabbit BM‐MSCs are CD73‐negative and CD90‐low positive exist, we suggest detailed information regarding antibodies used should always be given, especially where no species‐specific antibodies are used. On the other hand, in the case of negative results, many investigators do not include a positive control for the antibodies used.…”

Section: Discussionmentioning

confidence: 99%

Different RNA and protein expression of surface markers in rabbit amniotic fluid‐derived mesenchymal stem cells

Kováč

Vašíček

Kulíková

et al. 2017

Biotechnology Progress

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Over the years, there has been much confusion in defining molecular markers of mesenchymal stem cells (MSCs) for other than human species due to a lack of species-specific antibodies. Therefore, the aim of our study was to define rabbit amniotic fluid-derived mesenchymal stem cells (rAF-MSCs) and to reflect upon the current identification of AF-MSCs by providing a summary of detected surface markers in different species. The expression of rAF-MSC surface markers was analyzed at the protein and mRNA level. Flow cytometry analyses showed that rAF-MSCs were positive for CD29 and CD44, low positive for CD90, but negative for CD73, CD105, and CD166. Interestingly, RT-PCR (reverse transcription-polymerase chain reaction) exposed a discprepancy between transcribed mRNA and protein expression, as the cells expressed mRNA of all MSC markers: CD29, CD44, CD73, CD90, CD105, and CD166. Our results also confirmed the mesenchymal nature of isolated cells by morphology, ultrastructure, and intracellular marker expression profile. In addition, the expression of few pluripotency markers was also detected. We also found that passaging did not affect apoptosis and viability. Similarly, changes in karyotype were not observed during passaging. In conclusion, the provided characteristics may be used as a comprehensive set of criteria to define and characterize rAF-MSCs, required for the identification of these cells in preclinical investigations. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:1601-1613, 2017.

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Section: Discussionmentioning

confidence: 99%

Different RNA and protein expression of surface markers in rabbit amniotic fluid‐derived mesenchymal stem cells

Kováč

Vašíček

Kulíková

et al. 2017

Biotechnology Progress

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show abstract

“…The Mesenchymal and Tissue Stem Cell Committee of the International Society for Cellular Therapy proposes minimal criteria to define human MSCs: (a) cells must be plastic-adherent when maintained in standard culture conditions (5% CO2, 37 C); (b) high expression of CD105, CD73, and CD90, and low expression of CD45, CD34, CD14 or CD11b, CD79alpha or CD19, and HLA-DR surface molecules; and (c) MSCs must differentiate in vitro into osteoblasts, adipocytes, and chondroblasts (Dominici et al, 2006). However, MSCs human surface markers do not correspond to rabbit MSCs markers that were sporadically reported in literature (Lee et al, 2013). In current study, we did not employ the immunophenotyping but assessed morphology and differentiation properties of the cells.…”

Section: Isolation Of Bone Marrow-derived Mesenchymal Stromal Cellsmentioning

confidence: 99%

Urethroplasty with a bilayered poly‐D,L‐lactide‐co‐ε‐caprolactone scaffold seeded with allogenic mesenchymal stem cells

et al. 2019

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Reconstructive surgery for urethral defects employing tissue‐engineered scaffolds represents an alternative treatment for urethroplasty. The aim of this study was to compare the therapeutic efficacy of the bilayer poly‐D,L‐lactide/poly‐ε‐caprolactone (PL‐PC) scaffold seeded with allogenic mesenchymal stem cells (MSCs) for urethra reconstruction in a rabbit model with conventional urethroplasty employing an autologous buccal mucosa graft (BG). The inner layer of the scaffold based on poly‐D,L‐lactic acid (PL) was seeded with MSCs, while the outer layer, prepared from poly‐ε‐caprolactone, protected the surrounding tissues from urine. To track the MSCs in vivo, the latter were labeled with superparamagnetic iron oxide nanoparticles. In rabbits, a dorsal penile defect was reconstructed employing a BG or a PL‐PC graft seeded with nanoparticle‐labeled MSCs. In the 12‐week follow‐up period, no complications were detected. Subsequent histological analysis demonstrated biointegration of the PL‐PC graft with surrounding urethral tissues. Less fibrosis and inflammatory cell infiltration were observed in the experimental group as compared with the BG group. Nanoparticle‐labeled MSCs were detected in the urothelium and muscular layer, co‐localizing with the urothelium cytokeratin marker AE1/AE3, indicating the possibility of MSC differentiation into neo‐urothelium. Our results suggest that a bilayer MSCs‐seeded scaffold could be efficiently employed for urethroplasty.

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“…(BD Biosciences, cat#561956) were utilized for flow cytometric analysis following the manufacturer's instructions. CD44 and CD81 are previously reported MSC markers specific to rabbits [16][17][18][19]. Binding of antibodies against the markers was detected by anti-mouse immunoglobulin (IgG) conjugate, isotype-specific FITC or PE conjugated goat anti-mouse IgG (Becton Dickinson, Franklin Lakes, NJ).…”

Section: Plos Onementioning

confidence: 99%

Oxygen and mechanical ventilation impede the functional properties of resident lung mesenchymal stromal cells

Moreira¹,

Siddiqui²,

Macias³

et al. 2020

PLoS ONE

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Resident/endogenous mesenchymal stromal cells function to promote the normal development, growth, and repair of tissues. Following premature birth, the effects of routine neonatal care (e.g. oxygen support and mechanical ventilation) on the biological properties of lung endogenous mesenchymal stromal cells is (L-MSCs) is poorly understood. New Zealand white preterm rabbits were randomized into the following groups: (i) sacrificed at birth (Fetal), (ii) spontaneously breathing with 50% O 2 for 4 hours (SB), or (iii) mechanical ventilation with 50% O 2 for 4h (MV). At time of necropsy, L-MSCs were isolated, characterized, and compared. L-MSCs isolated from the MV group had decreased differentiation capacity, ability to form stem cell colonies, and expressed less vascular endothelial growth factor mRNA. Compared to Fetal L-MSCs, 98 and 458 genes were differentially expressed in the L-MSCs derived from the SB and MV groups, respectively. Gene ontology analysis revealed these genes were involved in key regulatory processes including cell cycle, cell division, and angiogenesis. Furthermore, the L-MSCs from the SB and MV groups had smaller mitochondria, nuclear changes, and distended endoplasmic reticula. Short-term hyperoxia/mechanical ventilation after birth alters the biological properties of L-MSCs and stimulates genomic changes that may impact their reparative potential.

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Characterization and spinal fusion effect of rabbit mesenchymal stem cells

Cited by 20 publications

References 22 publications

Different RNA and protein expression of surface markers in rabbit amniotic fluid‐derived mesenchymal stem cells

Different RNA and protein expression of surface markers in rabbit amniotic fluid‐derived mesenchymal stem cells

Urethroplasty with a bilayered poly‐D,L‐lactide‐co‐ε‐caprolactone scaffold seeded with allogenic mesenchymal stem cells

Oxygen and mechanical ventilation impede the functional properties of resident lung mesenchymal stromal cells

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