Characterization and screening of cyclooxygenase-2 inhibitors from Zi-shen pill by affinity ultrafiltration-ultra performance liquid chromatography mass spectrometry

Huai, Jiaxin; Zhao, Xiaoning; Wang, Siqi; Xie, Linlin; Li, Yiran; Zhang, Teng; Cheng, Congcong; Dai, Ronghua

doi:10.1016/j.jep.2019.111900

Cited by 21 publications

(6 citation statements)

References 30 publications

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“…Typically, an affinity ultrafiltration LC-MS screening involves three steps: incubation, screening by ultrafiltration and characterization by MS [ 42 ]. It is known that the optimization of conditions may increase the accuracy of the UF-UHPLC-ESI-QTOF-MS results [ 18 ].…”

Section: Discussionmentioning

confidence: 99%

Identification of Asiaticoside from Centella erecta (Apiaceae) as Potential Apyrase Inhibitor by UF-UHPLC-MS and Its In Vivo Antischistosomal Activity

et al. 2022

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Schistosomiasis, caused by parasites of the genus Schistosoma, is a neglected disease with high global prevalence, affecting more than 240 million people in several countries. Praziquantel (PZQ) is the only drug currently available for the treatment. S. mansoni NTPDases (known as SmNTPDases, ATP diphosphohydrolases or ecto-apyrases) are potential drug targets for the discovery of new antischistosomal drugs. In this study, we screen NTPDases inhibitors from Centella erecta (Apiaceae) using an ultrafiltration combined UHPLC-QTOF-MS method and potato apyrase, identifying asiaticoside as one of the apyrase-binding compounds. After isolation of asiaticoside from C. erecta extract, we assessed its in vivo antischistosomal activities against Schistosoma mansoni worms and its in vitro enzymatic apyrase inhibition. Also, molecular docking analysis of asiaticoside against potato apyrase, S. mansoni NTPDases 1 and 2 were performed. Asiaticoside showed a significant in vitro apyrase inhibition and molecular docking studies corroborate with its possible actions in potato apyrase and S. mansoni NTPDases. In mice harboring a patent S. mansoni infection, a single oral dose of asiaticoside (400 mg/kg. p.o.) showed significantly in vivo antischistosomal efficacy, markedly decreasing the total worm load and egg burden, giving support for further exploration of apyrase inhibitors as antischistosomal agents.

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Section: Discussionmentioning

confidence: 99%

Identification of Asiaticoside from Centella erecta (Apiaceae) as Potential Apyrase Inhibitor by UF-UHPLC-MS and Its In Vivo Antischistosomal Activity

et al. 2022

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“…Based on the importance of COX-2 for cancerology, six works employed this target in the development of ligand fishing assays to screen natural products, aiming to find innovative inhibitors. Four of the articles focused on the role of COX-2 inhibitors in cancer therapy [106][107][108][109], whereas two works focused on the anti-inflammatory features of the fished inhibitors [110,111]. In any case, COX-2 inhibitors can be extremely valuable in both scenarios.…”

Section: Cyclooxygenase-2mentioning

confidence: 99%

Ligand fishing as a tool to screen natural products with anticancer potential

Filho

Oliveira

Leal

et al. 2023

J of Separation Science

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Cancer is the second leading cause of death in the world and its incidence is expected to increase with the aging of the world's population and globalization of risk factors. Natural products and their derivatives have provided a significant number of approved anticancer drugs and the development of robust and selective screening assays for the identification of lead anticancer natural products are essential in the challenge of developing personalized targeted therapies tailored to the genetic and molecular characteristics of tumors. To this end, a ligand fishing assay is a remarkable tool to rapidly and rigorously screen complex matrices, such as plant extracts, for the isolation and identification of specific ligands that bind to relevant pharmacological targets. In this paper, we review the application of ligand fishing with cancer‐related targets to screen natural product extracts for the isolation and identification of selective ligands. We provide critical analysis of the system configurations, targets, and key phytochemical classes related to the field of anticancer research. Based on the data collected, ligand fishing emerges as a robust and powerful screening system for the rapid discovery of new anticancer drugs from natural resources. It is currently an underexplored strategy according to its considerable potential.

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“…Affinity ultrafiltration coupled with HPLC-MS was used to identified inhibitors, explore the structure-activity relationships. Moreover, investigators used nearly the same strategy to screen XO inhibitors such as galangal extract ( Lin et al, 2018 ) and Lindera reflexa Hemsl ( Fu et al, 2019b ), and Zishen pill ( Huai et al, 2019 , Wang et al, 2020b ) and Warburgia ugandensis ( Xie et al, 2020 ) for cyclooxygenase 2 (COX-2) and 5-LOX inhibitors, Azadirachta indica screening for superoxide dismutase inhibitors ( Fan et al, 2022 ), and herbal extracts screening for the identification of 5-HT2C receptor inhibitors ( Zhang et al, 2020a ), among others.…”

Section: Frontier Approaches and Technologies For Pharmacodynamic Sub...mentioning

confidence: 99%

Unraveling the mystery of efficacy in Chinese medicine formula: New approaches and technologies for research on pharmacodynamic substances

Lin

Wang

et al. 2022

Arabian Journal of Chemistry

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Characterization and screening of cyclooxygenase-2 inhibitors from Zi-shen pill by affinity ultrafiltration-ultra performance liquid chromatography mass spectrometry

Cited by 21 publications

References 30 publications

Identification of Asiaticoside from Centella erecta (Apiaceae) as Potential Apyrase Inhibitor by UF-UHPLC-MS and Its In Vivo Antischistosomal Activity

Identification of Asiaticoside from Centella erecta (Apiaceae) as Potential Apyrase Inhibitor by UF-UHPLC-MS and Its In Vivo Antischistosomal Activity

Ligand fishing as a tool to screen natural products with anticancer potential

Unraveling the mystery of efficacy in Chinese medicine formula: New approaches and technologies for research on pharmacodynamic substances

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