Characterization and role of poly(ADP-ribosyl)ation in the Mediterranean species Cistus incanus L. under different temperature conditions

Arena, Carmen; Mistretta, Carmela; Natale, Emiliana; Faraone-Mennella, Maria Rosaria; Santo, A. Vírzo De; Maio, Anna De

doi:10.1016/j.plaphy.2011.02.004

Cited by 21 publications

(36 citation statements)

References 35 publications

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“…PARP also affects the level of ABA and consequently ABA related signaling [44]. Down-regulation of PARP activity increases resistance against abiotic stresses such as temperature, excessive light and drought [27], [39], [44], [45] and this resistance is correlated with a reduced poly(ADP-ribose) level [39]. A similar situation was observed earlier in animal cells [46].…”

Section: Introductionsupporting

confidence: 58%

Chemical PARP Inhibition Enhances Growth of Arabidopsis and Reduces Anthocyanin Accumulation and the Activation of Stress Protective Mechanisms

et al. 2012

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Poly-ADP-ribose polymerase (PARP) post-translationally modifies proteins through the addition of ADP-ribose polymers, yet its role in modulating plant development and stress responses is only poorly understood. The experiments presented here address some of the gaps in our understanding of its role in stress tolerance and thereby provide new insights into tolerance mechanisms and growth. Using a combination of chemical and genetic approaches, this study characterized phenotypes associated with PARP inhibition at the physiological level. Molecular analyses including gene expression analysis, measurement of primary metabolites and redox metabolites were used to understand the underlying processes. The analysis revealed that PARP inhibition represses anthocyanin and ascorbate accumulation under stress conditions. The reduction in defense is correlated with enhanced biomass production. Even in unstressed conditions protective genes and molecules are repressed by PARP inhibition. The reduced anthocyanin production was shown to be based on the repression of transcription of key regulatory and biosynthesis genes. PARP is a key factor for understanding growth and stress responses of plants. PARP inhibition allows plants to reduce protection such as anthocyanin, ascorbate or Non-Photochemical-Quenching whilst maintaining high energy levels likely enabling the observed enhancement of biomass production under stress, opening interesting perspectives for increasing crop productivity.

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Section: Introductionsupporting

confidence: 58%

Chemical PARP Inhibition Enhances Growth of Arabidopsis and Reduces Anthocyanin Accumulation and the Activation of Stress Protective Mechanisms

et al. 2012

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“…The isolation of nuclei was performed according to Arena et al (2011). All operations were carried out on ice or at 4°C.…”

Section: Nuclei Isolationmentioning

confidence: 99%

“…The enzymatic activity was routinely assayed as reported in Arena et al (2011). The reaction mixture (final volume 50 μL) contained 0.5 M Tris-HCl pH 8.0, 50 mM MgCl 2 , 10 mM DTT, 0.4 mM [ 32 P] NAD+ (10,000 cpm/nmole) and a defined amount (20 μg protein) of whole nuclear fraction from examined tissues.…”

Section: Poly-adp-ribose Polymerase Standard Assaymentioning

confidence: 99%

“…Nuclear fractions (20 µg) from frog testes were electrophoresed onto 12% polyacrilammide mini-gel in 0.1% sodium dodecyl sulphate (SDS), according to Arena et al (2011). Staining was in 0.1% Comassie G in 10% acetic acid/30% methanol.…”

Section: Protein Analysis and Western Blottingmentioning

confidence: 99%

“…The filter was washed in TBST (Tris Buffered Saline with Tween) (1 × 5 min) and incubated in stripping buffer for 30 min at 50-60°C (in a heating oven). Washes in TBST (2 × 5 min) followed and membrane was blocked for 1 h in TBST 3% gelatine (Arena et al 2011).…”

Section: Protein Analysis and Western Blottingmentioning

confidence: 99%

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The amphibianPelophylax bergeri(Günther, 1986) testis poly(ADP-ribose)polymerases: relationship to endocrine disruptors during spermatogenesis

Maio

Trocchia

Guerriero

2014

Italian Journal of Zoology

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In a growing deterioration of the ecosystem both in terms of biodiversity and ecological features, even more problematic is the conservation of species. The aim of this work was to study seasonal variations of biochemical and molecular DNA damage markers in Pelophylax bergeri exposed to potential endocrine disrupting chemicals. Frogs were collected in the main phase of the reproductive cycle: in April and May, during active spermatogenesis and the breeding season, and in October and November, in spermatogenetic regression, from a sampling site in the polluted Sarno River and from a clean reference site (Matese Lake). DNA profile and poly(ADPribose) polymerase (PARP) activity were evaluated in the testis. In the main phases of the reproductive cycle, all specimens from Sarno River showed a PARP activity higher than that measured in the same frog's testis from Matese Lake. In addition, the PARP activity in active spermatogenesis was always higher compared with the activity in the spermatogenetic regression in Sarno River frogs. PARP2, usually activated in response to exposure to heavy metals, was expressed in all testes. In the species examined from Sarno River, no evident correlation between testis DNA damage and PARP activation was found. The working hypothesis is that PARP, implicated in genome surveillance and protection, might represent in frog spermatogenesis an appealing tool for genotoxic risk assessment useful to define a warning alarm for its survival.

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Proliferative events and apoptotic remodelling in retinal development of common toad (Bufo bufo)

et al. 2015

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Proliferation and apoptosis are fundamental processes in the development of the retina, and a proper balance of the two phenomena is crucial to correct development of the organ. Despite intense investigation in different vertebrates, only a few studies have analyzed the cell death and the cell division quantitatively in the same species during development. Here we studied the time course of apoptosis and proliferation in the retina of common toad, Bufo bufo, and discuss the findings in an evolutionary perspective. We found cells that were dividing first scattered throughout the retina, then, in later stages, proliferation was confined to the ciliary marginal zone. This pattern was confirmed by the expression of the proliferative marker PCNA. Both proliferation and apoptosis occurred in successive waves, and two apoptotic peaks were detected: one at premetamorphosis 1 and the second at prometamorphosis. PARP-1, a known molecular marker of apoptosis, was used to confirm the data obtained by counting pyknotic nuclei. In summary, proliferative and apoptotic waves display an inverse time-relationship through development, with apoptotic peaks coinciding with low proliferation phases. In a comparative perspective, amphibians follow a developmental pattern similar to other vertebrates, although with different timing.

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Characterization and role of poly(ADP-ribosyl)ation in the Mediterranean species Cistus incanus L. under different temperature conditions

Cited by 21 publications

References 35 publications

Chemical PARP Inhibition Enhances Growth of Arabidopsis and Reduces Anthocyanin Accumulation and the Activation of Stress Protective Mechanisms

Chemical PARP Inhibition Enhances Growth of Arabidopsis and Reduces Anthocyanin Accumulation and the Activation of Stress Protective Mechanisms

The amphibianPelophylax bergeri(Günther, 1986) testis poly(ADP-ribose)polymerases: relationship to endocrine disruptors during spermatogenesis

Proliferative events and apoptotic remodelling in retinal development of common toad (Bufo bufo)

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