Characterization and role in morphogenesis of a new subviral particle (55S) isolated from poliovirus-infected cells

Abstract

A previously undetected subviral particle, designated the 55S particle because of its position in sucrose density gradients, has been found in cytoplasmic extracts of poliovirus-infected cells. It contains no RNA, is composed of equimolar amounts of the structural polypeptides PlAB, P1C, and P1D, and is stable in vitro under a variety of conditions: presence or absence of EDTA, dilution in low-or high-ionic-strength buffers, suspension-in buffers up to pH 10, incubation at 37°C, and centrifugation to equilibrium in CsCl gradients (where it bands at a density of 1.285 g/cm3). Conventional pulse-chase experiments show that 55S particles are the products of the assembly of 14S subunits and the precursors of virions. These data led to the formulation of a model of poliovirus morphogenesis in which the conversion of capsomers into 73S empty capsids does not occur directly, but through the formation of an intermediate structure, the 55S particle.