Characterization and regulation of the 2,3-butanediol pathway in Serratia marcescens

Ben, Rao; Zhang, Liao Yuan; Sun, Jianan; Su, Gang; Wei, Dongzhi; Chu, Ju; Zhu, Junwen; Shen, Yang

doi:10.1007/s00253-011-3608-5

Cited by 47 publications

(39 citation statements)

References 23 publications

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“…In this organism, the genes encoding the first two steps of the pathway, budB and budA, respectively, are clustered in the budAB operon, while the third gene, budC, is at a distant site in the chromosome (6,11). This organization is similar to that found in Serratia marcescens (4), although the three genes can also form a single operon in some other members of the Enterobacteriaceae (12,13). Most recently, we demonstrated that loss of the budAB operon not only results in stronger acidification and thus earlier growth arrest in (neutral) glucose-containing media but also impairs the ability of S. plymuthica RVH1 to initiate and sustain growth at low pH (14).…”

supporting

confidence: 51%

“…On the other hand, enterobacteria that make use of the 2,3-butanediol fermentation pathway, such as Serratia, Klebsiella, or Enterobacter species, ferment glucose primarily to nonacidic end products, such as ethanol, acetoin, and 2,3-butanediol, and produce only minor amounts of the organic acids mentioned above (2). In fact, the latter bacteria follow the mixed-acid fermentation route during the early stages of growth and switch to the 2,3-butanediol route in the late-exponential phase (3,4). This switch is generally viewed as a strategy of these bacteria to prevent excessive medium acidification, and it has been demonstrated that knockout of the 2,3-butanediol pathway indeed results in more-pronounced acidification, early growth arrest, and cell death (5,6).…”

mentioning

confidence: 99%

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Acetoin Synthesis Acquisition Favors Escherichia coli Growth at Low pH

Vivijs

Moons

Aertsen

et al. 2014

Appl Environ Microbiol

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Some members of the family Enterobacteriaceae ferment sugars via the mixed-acid fermentation pathway. This yields large amounts of acids, causing strong and sometimes even lethal acidification of the environment. Other family members employ the 2,3-butanediol fermentation pathway, which generates comparatively less acidic and more neutral end products, such as acetoin and 2,3-butanediol. In this work, we equipped Escherichia coli MG1655 with the budAB operon, encoding the acetoin pathway, from Serratia plymuthica RVH1 and investigated how this affected the ability of E. coli to cope with acid stress during growth. Acetoin fermentation prevented lethal medium acidification by E. coli in lysogeny broth (LB) supplemented with glucose. It also supported growth and higher stationary-phase cell densities in acidified LB broth with glucose (pH 4.10 to 4.50) and in tomato juice (pH 4.40 to 5.00) and reduced the minimal pH at which growth could be initiated. On the other hand, the acetoin-producing strain was outcompeted by the nonproducer in a mixed-culture experiment at low pH, suggesting a fitness cost associated with acetoin production. Finally, we showed that acetoin production profoundly changes the appearance of E. coli on several diagnostic culture media. Natural E. coli strains that have laterally acquired budAB genes may therefore have escaped detection thus far. This study demonstrates the potential importance of acetoin fermentation in the ecology of E. coli in the food chain and contributes to a better understanding of the microbiological stability and safety of acidic foods.

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supporting

confidence: 51%

mentioning

confidence: 99%

Acetoin Synthesis Acquisition Favors Escherichia coli Growth at Low pH

Vivijs

Moons

Aertsen

et al. 2014

Appl Environ Microbiol

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“…The unknown QS-dependent bacterial determinant results in the increased susceptibility of AHL tobacco plants against bacterial and viral pathogen infection. For instance, a previous study indicated that QS in S. marcescens controlled 2,3-butanediol production by regulating the production of the precursor acetoin (Rao et al, 2012). Previously 2,3-butanediol as a bacterial determinant induced to up-regulate JA signaling-related genes such as PDF1.2 (Ryu et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Modulation of Quorum Sensing in Acyl-homoserine Lactone-Producing or -Degrading Tobacco Plants Leads to Alteration of Induced Systemic Resistance Elicited by the Rhizobacterium Serratia marcescens 90-166

Ryu¹,

Choi²,

Lee³

et al. 2013

The Plant Pathology Journal

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Numerous root-associated bacteria (rhizobacteria) are known to elicit induced systemic resistance (ISR) in plants. Bacterial cell-density-dependent quorum sensing (QS) is thought to be important for ISR. Here, we investigated the role of QS in the ISR elicited by the rhizobacterium, Serratia marcescens strain 90−166, in tobacco. Since S. marcescens 90−166 produces at least three QS signals, QS-mediated ISR in strain 90−166 has been difficult to understand. Therefore, we investigated the ISR capacity of two transgenic tobacco (Nicotiana tabacum) plants that contained either bacterial acylhomoserine lactone-producing (AHL) or -degrading (AiiA) genes in conjunction with S. marcescens 90−166 to induce resistance against bacterial and viral pathogens. Root application of S. marcescens 90−166 increased ISR to the bacterial pathogens, Pectobacterium carotovorum subsp. carotovorum and Pseudomonas syringae pv. tabaci, in AHL plants and decreased ISR in AiiA plants. In contrast, ISR to Cucumber mosaic virus was reduced in AHL plants treated with S. marcescens 90−166 but enhanced in AiiA plants. Taken together, these data indicate that QS-dependent ISR is elicited by S. marcescens 90−166 in a pathogen-dependent manner. This study provides insight into QS-dependent ISR in tobacco elicited by S. marcescens 90−166.

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“…Sodium acetate as an inducer could increase the transcription level of 2,3-BD pathway in S. marcescens H30 and other 2,3-BD producing microbial strains (Zeng et al 1990;Ji et al 2011;Rao et al 2012), and further result in 2,3-BD accumulation during the fermentation process. The use of KH2PO4 could provide a phosphorus source for the growth of 2,3-BD producing strains (Zhang et al 2010a;Sun et al 2012).…”

Section: Effects Of Inorganic Salts On 23-bd Fermentationmentioning

confidence: 99%

Utilization of Sweet Sorghum Juice for Efficient 2,3-Butanediol Production by Serratia marcescens H30

Yuan¹,

He²,

Guo³

et al. 2017

BioResources

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Sweet sorghum juice (SSJ) is considered a good carbon source for biorefinery due to its low price and high fermentable-sugar content. In this study, 2,3-butanediol (2,3-BD) production from SSJ by Serratia marcescens H30 was investigated. First, the medium compositions including the contents of SSJ, nitrogen source, and mineral salts were optimized in conical flasks using a single factor and orthogonal design method. Under the optimal conditions, the 2,3-BD concentration reached up to 33.40 g/L. Then the optimized medium was used to perform fermentative experiments in a 5-L bioreactor. In batch experiments, the effects of various agitation speeds on 2,3-BD production were compared. Based on batch process results, an efficient two-stage fermentative control strategy was developed, where the agitation speed was maintained at 300 rpm in the first 12 h and subsequently switched to 200 rpm. About 43.32 g/L 2,3-BD was obtained by using this strategy. Finally, fed-batch fermentation was conducted through feeding the concentrated SSJ and a maximum 2,3-BD concentration of 109.44 g/L with the productivity of 1.40 g/L·h; a yield of 83.02% was achieved. The results showed that SSJ could be used as an economical substrate for efficient 2,3-BD production by S. marcescens H30.

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Characterization and regulation of the 2,3-butanediol pathway in Serratia marcescens

Cited by 47 publications

References 23 publications

Acetoin Synthesis Acquisition Favors Escherichia coli Growth at Low pH

Acetoin Synthesis Acquisition Favors Escherichia coli Growth at Low pH

Modulation of Quorum Sensing in Acyl-homoserine Lactone-Producing or -Degrading Tobacco Plants Leads to Alteration of Induced Systemic Resistance Elicited by the Rhizobacterium Serratia marcescens 90-166

Utilization of Sweet Sorghum Juice for Efficient 2,3-Butanediol Production by Serratia marcescens H30

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