Characterization and Quantitation of Anthocyanins and Other Phenolics in Native Andean Potatoes

Giusti, M. Mónica; Polit, Maria Fernanda; Ayvaz, Hüseyin; Tay, David; Manrique, Ivan

doi:10.1021/jf500655n

Cited by 63 publications

(40 citation statements)

References 20 publications

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“…The increase in reflectance starting at 250 nm of barley leaves treated with 80g NaCl/l phytoagar from 4 dai onward is also in accordance with chlorosis symptoms visible at 4 dai. The constant vitality of untreated barley leaves 1-5 dai was in accordance with similar reflectance patterns and can be linked to consistent amounts of plant molecules absorbing in the UV-range like flavonoids, amino acids, anthocyanins [8][9][10]. Absorption features are represented by values smaller than one [31] and were mainly seen from 320 nm onward.…”

Section: Discussionsupporting

confidence: 54%

“…The ultraviolet range (200-380 nm ) has not yet been used in plant phenotyping. A number of plant molecules like flavonoids, amino acids, anthocyanins and nucleoside feature absorption maxima in the UV-range [8][9][10]. The aromatic compound phenol for example shows a maximum absorption at 270-274 nm and is produced by a multiplicity of plants as protection against stress [11].…”

Section: Introductionmentioning

confidence: 99%

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Extending Hyperspectral Imaging for Plant Phenotyping to the UV-Range

Brugger

Behmann

Paulus³

et al. 2019

Remote Sensing

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Previous plant phenotyping studies have focused on the visible (VIS, 400–700 nm), near-infrared (NIR, 700–1000 nm) and short-wave infrared (SWIR, 1000–2500 nm) range. The ultraviolet range (UV, 200–380 nm) has not yet been used in plant phenotyping even though a number of plant molecules like flavones and phenol feature absorption maxima in this range. In this study an imaging UV line scanner in the range of 250–430 nm is introduced to investigate crop plants for plant phenotyping. Observing plants in the UV-range can provide information about important changes of plant substances. To record reliable and reproducible time series results, measurement conditions were defined that exclude phototoxic effects of UV-illumination in the plant tissue. The measurement quality of the UV-camera has been assessed by comparing it to a non-imaging UV-spectrometer by measuring six different plant-based substances. Given the findings of these preliminary studies, an experiment has been defined and performed monitoring the stress response of barley leaves to salt stress. The aim was to visualize the effects of abiotic stress within the UV-range to provide new insights into the stress response of plants. Our study demonstrated the first use of a hyperspectral sensor in the UV-range for stress detection in plant phenotyping.

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Section: Discussionsupporting

confidence: 54%

Section: Introductionmentioning

confidence: 99%

Extending Hyperspectral Imaging for Plant Phenotyping to the UV-Range

Brugger

Behmann

Paulus³

et al. 2019

Remote Sensing

View full text Add to dashboard Cite

show abstract

“…Other anthocyanins found were pelargonidin caffeoylrutinoside-5-glucoside (6.82%) and pelargonidin-3-ferroylrutiniside-5-glucoside (4.5%). Some previous reports described the presence of such group anthocyanin compounds in red and purple potatoes [ 13 , 26 – 28 ]. However, the availability and the relative abundancy of these compounds depends on cultivars and agronomic conditions [ 13 , 26 – 28 ].…”

Section: Resultsmentioning

confidence: 99%

Inhibition of α-glucosidase, α-amylase, and aldose reductase by potato polyphenolic compounds

et al. 2018

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Diabetes mellitus is a chronic disease that is becoming a serious global health problem. Diabetes has been considered to be one of the major risks of cataract and retinopathy. Synthetic and natural product inhibitors of carbohydrate degrading enzymes are able to reduce type 2 diabetes and its complications. For a long time, potatoes have been portrayed as unhealthy for diabetic patients by some nutritionist due to their high starch content. However, purple and red potato cultivars have received considerable attention from consumers because they have high levels of polyphenolic compounds that have potent antioxidant activities. In this study, we screened the total phenolics (TP) and total anthocyanins (TA) and analyzed the phenolic and anthocyanin compounds in selected potato cultivars and advanced selections with distinct flesh colors (purple, red, yellow and white). Purple and red potato cultivars had higher levels of TP and TA than tubers with other flesh colors. Chlorogenic acid is the predominant phenolic acid, and major anthocyanin is composed of the derivatives of petunidin, peonidin, malvidin and pelargonidin. We tested the potential inhibitory effect of potato extracts on the activities of α-amylase and α-glucosidase, which were targeted to develop antidiabetic therapeutic agents. We also measured inhibitory effect of potato extracts on aldose reductase (AR) which is a key enzyme that has been a major drug target for the development of therapies to treat diabetic complications. Purple flesh tubers extract showed the most effective inhibition of α-amylase, α-glucosidase, and aldose reductase with IC50 values 25, 42, and 32 μg/ml, respectively. Kinetic studies showed that anthocyanins are noncompetitive inhibitors of these enzymes, whereas phenolic acids behaved as mixed inhibitors for α-amylase and α-glucosidase and noncompetitive inhibitors for AR. This study supports the development of a positive and healthful image of potatoes, which is an important issue for consumers.

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“…The majority of anthocyanins in potatoes have been shown to possess the structure: anthocyanidin 3-acyl-rutinoside-5-glucoside. The most common acyl substitutes are caffeoyl, p- coumaroyl or feruloyl residues [ 36 , 37 ]. This is in agreement with the data in Table 1 where these anthocyanin structures were predominant in V1 for both tested potato cultivars.…”

Section: Resultsmentioning

confidence: 99%

Effects of Simulated Human Gastrointestinal Digestion of Two Purple-Fleshed Potato Cultivars on Anthocyanin Composition and Cytotoxicity in Colonic Cancer and Non-Tumorigenic Cells

et al. 2017

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A dynamic human gastrointestinal (GI) model was used to digest cooked tubers from purple-fleshed Amachi and Leona potato cultivars to study anthocyanin biotransformation in the stomach, small intestine and colonic vessels. Colonic Caco-2 cancer cells and non-tumorigenic colonic CCD-112CoN cells were tested for cytotoxicity and cell viability after 24 h exposure to colonic fecal water (FW) digests (0%, 10%, 25%, 75% and 100% FW in culture media). After 24 h digestion, liquid chromatography-mass spectrometry identified 36 and 15 anthocyanin species throughout the GI vessels for Amachi and Leona, respectively. The total anthocyanin concentration was over thirty-fold higher in Amachi compared to Leona digests but seven-fold higher anthocyanin concentrations were noted for Leona versus Amachi in descending colon digests. Leona FW showed greater potency to induce cytotoxicity and decrease viability of Caco-2 cells than observed with FW from Amachi. Amachi FW at 100% caused cytotoxicity in non-tumorigenic cells while FW from Leona showed no effect. The present findings indicate major variations in the pattern of anthocyanin breakdown and release during digestion of purple-fleshed cultivars. The differing microbial anthocyanin metabolite profiles in colonic vessels between cultivars could play a significant role in the impact of FW toxicity on tumor and non-tumorigenic cells.

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Characterization and Quantitation of Anthocyanins and Other Phenolics in Native Andean Potatoes

Cited by 63 publications

References 20 publications

Extending Hyperspectral Imaging for Plant Phenotyping to the UV-Range

Extending Hyperspectral Imaging for Plant Phenotyping to the UV-Range

Inhibition of α-glucosidase, α-amylase, and aldose reductase by potato polyphenolic compounds

Effects of Simulated Human Gastrointestinal Digestion of Two Purple-Fleshed Potato Cultivars on Anthocyanin Composition and Cytotoxicity in Colonic Cancer and Non-Tumorigenic Cells

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