Characterization and Quantification of Siderophores Produced by Aeromonas hydrophila Isolated from Cyprinus carpio

Murugappan, R. M.; Rekha, S.; Thirumurugan, R.

doi:10.3923/pjbs.2006.437.440

Cited by 6 publications

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“…Estas enterotoxinas también se han descrito por los investigadores como -hemolisinas y aerolisinas. Otros factores de virulencia reportados para A. hydrophila son: la producción de proteasas que contribuyen a la utilización de nutrientes para la proliferación celular y que causan daño al tejido, presencia de adhesinas, pili, flagelina, la presencia de moléculas secuestradoras de hierro denominas sideróforos (ligando específico de hierro de bajo peso molecular) (Murugappan et al, 2006), y la presencia de la capa S, arreglos de un tipo de proteína de superficie que forman una estructura supramolecular que encierra la bacteria y permite la interacción con tejidos y fluidos corporales, influencia la interacción hospedero-patógeno y se ha probado que su presencia aumenta la letalidad en peces (Kostrzynska et al, 1992).…”

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Estudio clínico y hematológico de una infección experimental con Aeromonas hydrophila y Edwardsiella tarda en tilapia, Oreochromis sp

et al. 2010

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Titulo en ingles: Clinical and hematological study of experimental infection with Aeromonas hydrophila and Edwardsiella tarda on tilapia Oreochromis sp.RESUMEN: Las bacterias oportunistas como A. hydrophila y E. tarda se relacionan con brotes de septicemia hemorrágica en tilapias de cultivo en nuestro país, siendo los agentes de mayor participación en enfermedades infecciosas en cultivos de Oreochromis sp en el Departamento del Meta. Este artículo reporta un estudio clínico y hematológico de tilapias híbridas (50 - 70g) durante la infección experimental con Aeromonas hydrophila y Edwardsiella tarda; realizada por medio de inoculaciones intraperitoneales de 7 x 108 UFC/ml y 2 x 108 UFC/ml, de A. hydrophila y E. tarda respectivamente. A partir de las 11 horas los peces infectados presentaron: palidez, letargo, boqueo, aumento de la frecuencia opercular y pérdida de eje de nado. En el examen hematológico, los animales infectados presentaron linfopenia severa, aumento de monocitos, hemólisis, abundantes células blásticas, marcada carriorrexis, hipocromía y anisocitosis, características de una anemia hipocrómica y eritroclasia descrita en infecciones bacterianas en peces. Al examen de necropsia, los peces inoculados con las bacterias evidenciaron: hemorragias en la base de las aletas y órganos internos, palidez e hígado moteado, aumento de líquido abdominal y líquido sanguinolento en el intestino, a diferencia de animales control que no presentaron estas lesiones. Adicionalmente, se observó hepatomegalia en animales infectados, aunque no hubo diferencias significativ as en el índice hepatosomático de estos animales con respecto a animales control. En el tratamiento de E. tarda, en el bazo se observaron nódulos blanquecinos de distribución multifocal. Durante el presente estudio tilapias hibridas infectadas con E. tarda y A. hydrophila, mostraron un cuadro clínico compatible con septicemia hemorrágica, posiblemente ocasionado por a productos extracelulares (PEC) tales como hemolisinas y aerolisinas con actividad hemolítica y citotóxica en peces, entre otros factores. Este trabajo constituye una aproximación a un modelo de estudio de enfermedad bacteriana de tilapias con cepas patógenas aisladas en la región.Palabras clave: Bacteria, hematología, septicemia, tilapia híbrida.ABSTRACT: Opportunistic bacteria as A. hydrophila and E. tarda are related to haemorrhagic septicaemia outbreaks in cultured tilapias in our country, being the aetiological agents of highest participation on the diagnosis of infection disease in cultures of Oreochromis sp., in Departamento del Meta. This article reports a clinical and hematological study of tilapia hybrid (50-70g) during the experimental infection with Aeromonas hydrophila and Edwardsiella tarda made by intraperitoneal inoculation of 7 x 108 CFU/ml and 2 x 108 CFU/ ml of A. hydrophila and E. tarda, respectively. From 11 hours infected fish showed paleness, lethargy, gasping, increased respiratory frequency and loss of swim axis. At hematological examination, infected animals showed severe lymphopenia, increased monocytes, hemolysis, increased blastic cells, karyor- rhexis, hypochromia and anisocytosis, which is characteristic of a hypochromic anemia and erythroclasia described in fish bacterial infection. At necropsy examination, fish inoculated with bacteria showed: hem- orrhages on the base of fins and internal organs, pale and mottled liver, increased abdominal fluid and blood-stained fluid in intestine, unlike control animals that did not show these lesions. Additionally, hepatome- galy was observed in experimental infected animals, even though there were not significance differences in the hepatosomatic index of these animals with respect to control. In treatment of E. tarda, whitish nodules with multifocal distribution were observed in spleen. During the present study, hybrid tilapias infected with E. tarda y A. hydrophila, showed a clinical condition compatible with haemorrhagic septicaemia, possibly associated to extracellular products (ECP), such as hemolysins and aerolisyns, with hemolytic and cyto- toxic activity in fish, among other factors. This work is an approximation to a model of study of bacterial disease of tilapia with pathogenic strains isolated in the region.Key words: Bacteria, hematology, septicaemia, Oreochromis sp., tilapia hybrid.

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Section: Introductionunclassified

Estudio clínico y hematológico de una infección experimental con Aeromonas hydrophila y Edwardsiella tarda en tilapia, Oreochromis sp

et al. 2010

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Role of Microorganisms in Plant Nutrition and Health

Prakash

Sharma

Rahi

et al. 2014

Nutrient Use Efficiency: From Basics to Advances

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Chemical and structural characterization of hydroxamate siderophore produced by marine Vibrio harveyi

Murugappan

Aravinth

Karthikeyan³

2010

J Ind Microbiol Biotechnol

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In the present study, 22 different bacteria were isolated from open ocean water from the Gulf of Mannar, India. Of the 22 isolates, 4 were identified as Vibrio spp. (VM1, VM2, VM3 and VM4) and found to produce siderophores (iron-binding chelators) under iron-limited conditions. Different media were found to have an influence on siderophore production. Maximum siderophore production was observed with VM1 isolate in MM9 salts medium at 48 h of incubation. The isolate was confirmed as Vibrio harveyi based on 16S rRNA gene sequencing and phylogenetic analysis. Fourier-transform infrared (FTIR) and (1)H nuclear magnetic resonance (NMR) spectra revealed the hydroxamate nature of the siderophore produced. Further characterization of the siderophore revealed it to be of dihydroxamate nature, forming hexadentate ligands with Fe(III) ions. A narrow shift in ultraviolet (UV)-Vis spectrum was observed on photolysis due to ligand oxidation. Growth-promotion bioassay with Aeromonas hydrophila, Staphylococcus aureus and E. coli confirmed the iron-scavenging property of the siderophore produced by Vibrio harveyi.

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Characterization and Quantification of Siderophores Produced by Aeromonas hydrophila Isolated from Cyprinus carpio

Cited by 6 publications

References 0 publications

Estudio clínico y hematológico de una infección experimental con Aeromonas hydrophila y Edwardsiella tarda en tilapia, Oreochromis sp

Estudio clínico y hematológico de una infección experimental con Aeromonas hydrophila y Edwardsiella tarda en tilapia, Oreochromis sp

Role of Microorganisms in Plant Nutrition and Health

Chemical and structural characterization of hydroxamate siderophore produced by marine Vibrio harveyi

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