Characterization and Quantification of Polyphenols in Fruits

Weber, Fabian; Schulze-Kaysers, Nadine; Schieber, Andreas

doi:10.1016/b978-0-12-397934-6.00015-2

Cited by 6 publications

(10 citation statements)

References 69 publications

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“…The selection of the appropriate analytical strategy to study PCs in plant matrices depends on the purpose of the study, including the nature of the sample and the target compounds. Among the methods used for determination of PCs are those aimed to a general characterization of the samples, which measure the total content of PCs (TPC) or quantify a specific group or class . However, these analytical techniques have some drawbacks such as the overestimation of the phenolic concentration and the lack of quali/quantitative information on the individual PCs .…”

Section: Analytical Methods For Characterization Of Phenolicsmentioning

confidence: 99%

Background and Perspectives on the Utilization of Canes’ and Bunch Stems’ Residues from Wine Industry as Sources of Bioactive Phenolic Compounds

Ferreyra

Fontana

2023

J. Agric. Food Chem.

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Viticulture activity produces a significant amount of grapevine woody byproducts, such as bunch stems and canes, which constitute potential sources of a wide range of phenolic compounds (PCs) with purported applications. Recently, the study of these byproducts has been increased as a source of health-promoting phytochemicals. Antioxidant, antimicrobial, antifungal, and antiaging properties have been reported, with most of these effects being linked to the high content of PCs with antioxidant properties. This Review summarizes the data related to the qualitative and quantitative composition of PCs recovered from canes and bunch stems side streams of the wine industry, the influence that the different environmental and storage conditions have on the final concentration of PCs, and the current reported applications in specific technological fields. The objective is to give a complete valuation of the key factors to consider, starting from the field to the final extracts, to attain the most suitable and stable characterized product.

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Section: Analytical Methods For Characterization Of Phenolicsmentioning

confidence: 99%

Background and Perspectives on the Utilization of Canes’ and Bunch Stems’ Residues from Wine Industry as Sources of Bioactive Phenolic Compounds

Ferreyra

Fontana

2023

J. Agric. Food Chem.

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“…Several analytical methods are routinely used to determine a "total" polyphenol value for fruit juices and beverages. The Folin-Ciocalteu (F-C), Lowenthal permanganate (L-P), 4dimethylaminocinnamaldehyde (DMAC), and bovine serum albumin (BSA) precipitation methods are historically relevant and represent four of the most widely applied methods used in fruit juice and beverage analysis (Weber et al, 2014). Other relevant methods for polyphenol quantification include reading absorbance at 280 nm, precipitation with methyl cellulose, Prussian Blue, Bate-Smith, and Vanillin test (Aleixandre-Tudo et al, 2017).…”

Section: Analytical Methods For Polyphenol Quantificationmentioning

confidence: 99%

“…Although the strengths and weaknesses of these methods have been discussed in the literature (Aleixandre-Tudo et al, 2017;Weber et al, 2014), information on the analytical method validation parameters and the quantitative performance of four analytical methods (F-C, L-P, DMAC, and BSA) were not comprehensive. The objective of this study was to determine analytical method validation parameters and assess quantitative performance of four analytical methods (F-C, L-P, DMAC, and BSA) using a broad range of fruit juice and beverage samples to allow assessment of the influence of differences in polyphenol composition and sample matrix among fruit types (for example, berries, citrus, and apples) on quantitative results.…”

Section: Analytical Methods For Polyphenol Quantificationmentioning

confidence: 99%

“…Over 8000 polyphenols of plant origin have been characterized (Pandey & Rizvi, 2009). All polyphenols contain hydroxylated phenyl moieties, often present as glycosides (Weber, Schulze-Kaysers, & Schieber, 2014). Polyphenols are classified into flavonoids, phenolic acids, and other classes, including stilbenes and lignans based on their aglycone structures (Tsao, JFDS-2019-0450 Submitted 3/28/2019, Accepted 6/6/2019.…”

Section: Introduction Polyphenols In Fruit Juices and Beveragesmentioning

confidence: 99%

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Comparison of Common Analytical Methods for the Quantification of Total Polyphenols and Flavanols in Fruit Juices and Ciders

Kim

Neilson

et al. 2019

Journal of Food Science

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Multiple analytical methods are used for quantification of total polyphenols and total flavanols in fruit juices and beverages. Four methods were evaluated in this study: Folin‐Ciocalteu (F‐C), Lowenthal permanganate (L‐P), 4‐dimethylaminocinnamaldehyde (DMAC), and the bovine serum albumin (BSA) precipitation method. Method validation parameters, including working range, limit of detection, limit of quantitation, precision (repeatability), accuracy, and specificity, were assessed and compared. The F‐C method was not specific to polyphenols, and the L‐P method had the widest working range but lacked accuracy. The DMAC method was the most specific to flavanols, and the BSA method was not suitable for quantification of smaller flavanols, such as catechin and epicatechin. Quantitative performance was evaluated using commercial fruit juice samples (n = 14), apple juice samples of different cultivars (n = 22), and commercial ciders (n = 17). In general, the L‐P titration method and DMAC method resulted in higher quantitative values than the F‐C method and BSA precipitation method, respectively. However, ratios of results obtained by the L‐P and F‐C method ranged from 1 to 28, and ratios of results obtained by the DMAC and BSA precipitation method ranged from <1 to 280. This tremendous variation is likely due to variation in polyphenol composition and sample matrix. This information provides perspective for comparison of results obtained through these different methods, and a basis for choosing the most appropriate analytical method for quantification of polyphenols to address a specific research question when working with commercial fruit juice, apple juice from different apple cultivars, and commercial ciders. Practical Application This study compared results obtained when four common polyphenol quantification methods were applied to a diverse selection of fruit juices and beverages with distinct polyphenol composition and sample matrix. The matrix and polyphenol composition of the samples significantly influenced the results. Our findings can help manufacturers of fruit‐based products choose the most appropriate analytical method for polyphenol quantification as part of a quality assurance program or to convey information on dietary polyphenol content to consumers. An assessment of analytical method validation parameters is provided for each of the four methods, which will help users of these methods to understand their limitations.

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“…In nature, B-type compounds are more abundant than A-type ones. Regardless, A-type compounds possess great antimicrobial activity so that they are very useful for the prevention and treatment of urinary tract infections (UTIs) such as cystitis [12][13][14][15]. Currently, the 4-dimethylaminocinnamaldehyde (DMAC) spectrophotometric assay is the most popular method used for the quantification of overall PAC contents in nutraceuticals and functional foods.…”

Section: Introductionmentioning

confidence: 99%

Liquid Chromatographic Fingerprints for the Characterization of Flavanol-Rich Nutraceuticals Based on 4-Dimethylaminocinnamaldehyde Precolumn Derivatization

2021

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Flavanols consist of a great family of bioactive molecules displaying a wide range of health-promoting attributes for humans, including antioxidant, antimicrobial or anti-inflammatory effects. As a result, botanical species rich in this type of compound are often used to develop nutraceutical products or dietary supplements with recognized healthy attributes. This paper aims at characterizing nutraceutical products using liquid chromatographic fingerprints related to flavanol composition. Catechins and their oligomers were exploited to characterize and authenticate various commercial products prepared with extracts of red berries and medicinal plants. These compounds resulted in interesting descriptors of some fruits and vegetables, thus providing an additional perspective for the study of nutraceuticals. For such a purpose, a new method based on liquid chromatography with UV/Vis detection (HPLC–UV/Vis) with precolumn derivatization with 4-dimethylaminocinnamaldehyde was developed. Results indicated that the separation of flavanols was very complex due to the degradation of procyanidin derivatives. The resulting data sets were analyzed using chemometric methods such as principal component analysis and partial least square–discriminant analysis. Despite the complexity of chromatographic fingerprints, nutraceutical samples could be discriminated according to their main ingredients. In general, catechin and epicatechin were the most abundant compounds in the different samples, and procyanidin A2 was highly specific to cranberry.

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Characterization and Quantification of Polyphenols in Fruits

Cited by 6 publications

References 69 publications

Background and Perspectives on the Utilization of Canes’ and Bunch Stems’ Residues from Wine Industry as Sources of Bioactive Phenolic Compounds

Background and Perspectives on the Utilization of Canes’ and Bunch Stems’ Residues from Wine Industry as Sources of Bioactive Phenolic Compounds

Comparison of Common Analytical Methods for the Quantification of Total Polyphenols and Flavanols in Fruit Juices and Ciders

Liquid Chromatographic Fingerprints for the Characterization of Flavanol-Rich Nutraceuticals Based on 4-Dimethylaminocinnamaldehyde Precolumn Derivatization

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