Characterization and quantification of mRNA transcripts in ejaculated spermatozoa of fertile men by serial analysis of gene expression

Zhao, Yiwei; Li, Qiaoli; Yao, Chenjiang; Wang, Zhaoxia; Zhou, Yin; Wang, Yajing; Liu, Limin; Wang, Yifei; Wang, Lianyun; Qiao, Zhongdong

doi:10.1093/humrep/del027

Cited by 106 publications

(64 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The various transcripts that are present in spermatozoa and affect different metabolic pathways or functions raise many questions about the complexity of spermatogenesis and the significance of the transcripts that are generated. We are currently witnessing the emergence of a myriad of diverse transcripts identified using different transcript profiling platforms (Mao et al 2004, Dadoune et al 2005, Zhang et al 2006, Zhao et al 2006, Gilbert et al 2007, Lalancette et al 2008b.…”

Section: Introductionmentioning

confidence: 99%

Spermatozoal transcriptome profiling for bull sperm motility: a potential tool to evaluate semen quality

Bissonnette¹,

Lévesque-Sergerie²,

Thibault³

et al. 2009

Reproduction

View full text Add to dashboard Cite

Regarding bull fertility, establishing an association between in vitro findings and field fertility requires a multi-parametric approach that measures the integrity of various structures and dynamic functions, such as motion characteristics, among others. The heterogeneous RNA pattern of spermatozoa could be used in genomic analysis for evaluating both spermatogenesis and fertility potential of semen, mainly because of the static status of the transcriptome of this particular differentiated cell. In a previous study, we determined that some spermatozoal transcripts identified by PCR-based cDNA subtraction are associated with non-return rate, a field fertility index. In the present study, the microarray technology was used in conjunction with differential RNA transcript extraction. We have shown that among these genes, some transcripts are also associated with the motility status of a population of sperm cells fractionated from the same ejaculate. We highlighted a systematic data analysis and validation scheme important for the identification of significant transcripts in this context. With such an approach, we found that transcripts encoding a serine/threonine testis-specific protein kinase (TSSK6) and a metalloproteinase non coding RNA (ADAM5P ) are associated with high-motility status (P!0.001), also confirmed by quantitative PCR (PZ0.0075). This association was found only when transcripts were extracted using the hot-TRIzol protocol, whereas the cold-TRIzol RNA extract comprised mitochondrial transcripts. These results demonstrate that some transcripts previously identified in association with field fertility are also found associated with in vitro motility provided that a stringent RNA extraction protocol is used.

show abstract

Section: Introductionmentioning

confidence: 99%

Spermatozoal transcriptome profiling for bull sperm motility: a potential tool to evaluate semen quality

Bissonnette¹,

Lévesque-Sergerie²,

Thibault³

et al. 2009

Reproduction

View full text Add to dashboard Cite

show abstract

“…However, recent evidence suggests that a complex specific mRNA population is retained in freshly ejaculated spermatozoa of several species [1][2][3][4]. In addition, some spermatozoa mRNAs are transferred into the oocyte during fertilization, and remain stable until the activation of the embryonic genome [5].…”

mentioning

confidence: 99%

The role of Dby mRNA in early development of male mouse zygotes

Yao¹,

Xu²,

Gong³

et al. 2010

Asian J Androl

View full text Add to dashboard Cite

Ejaculated mammalian spermatozoa contain a complex yet specific population of mRNA. However, the possible roles that mRNA has in early zygotic and embryonic development remain unclear. We found that Dby mRNA is selectively retained in capacitated mouse spermatozoa, and is transferred into the oocyte during fertilization by reverse transcription-polymerase chain reaction even though no DBY protein expression is detected. The cellular location of Dby mRNA is seen in the post-acrosome region, and it comprises nearly half of the mouse spermatozoa in in situ hybridization. In contrast, transcripts of the control gene, Smcy, are not detected in capacitated mouse spermatozoa, although the H-Y antigen encoded by Smcy is expressed on the surface of the spermatozoa. In our microinjection experiment, the zygotic development rate of the as-Dby male pronucleus injection group was significantly lower than that of the as-Smcy male pronucleus injection group (35.9% vs. 95%, P = 0.001) and the as-Dby female pronucleus injection group (35.9% vs. 93.8%, P = 0.001). The rate of male-developed zygotes was also lower than that of the as-Smcy male pronucleus injection group (17.4% vs. 57.9%, P = 0.002) and the as-Dby female pronucleus injection group (17.4% vs. 54.1%, P = 0.002). Thus, we conclude that Dby mRNA is selectively retained in capacitated mouse spermatozoa, and it has an important role in the early zygotic development of male mouse zygotes. This might imply that spermatozoa mRNA is involved in early zygotic and embryonic stages of reproduction.

show abstract

“…Both have concluded that the mRNA species present within sperm must be either from past events; the gene expression of spermatozogenesis, or for providing the zygote with unique suite of paternal mRNAs. [14][15][16][17] In contrast to transcriptomics, the use of proteomics in sperm biology plays a unique, and arguably, pivotal role unseen in any other tissue/cell type beside blood. However, unlike blood, two important parameters make sperm cell an outstanding candidate for proteomic analysis.…”

Section: The Most Profound Statements Are Often Spoken In Silencementioning

confidence: 99%

The ’omics revolution and our understanding of sperm cell biology

Baker

2010

Asian J Androl

View full text Add to dashboard Cite

The foundations of proteomics are to study gene products and their regulatory roles within cells. Paradoxically, the only evidence that sperm cells make new proteins is through mitochondrial protein synthesis. Yet despite this, spermatozoa are the perfect candidates for mass spectrometry and hence, proteomic analysis. These enterprising cells use a plethora of post-translational modifications in order to gain functionality following their production within the testis. By using a combination of two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), and more recently liquid chromatography-mass spectrometry (LC-MS)/MS, recent advances in sperm cell biology, through the use of proteomics, is making unparalleled progress. The protein inventory lists being generated have shed light on transmembrane proteins, kinases and chaperones never previously recognized. In addition, the ability to isolate either phosphopeptides or glycopeptides and quantify the differences between cells of two different populations make proteomic analysis of spermatozoa a real chance to finally answer some age old questions.

show abstract

Characterization and quantification of mRNA transcripts in ejaculated spermatozoa of fertile men by serial analysis of gene expression

Abstract: A SAGE analysis of ejaculate from fertile men has revealed a large number of transcripts, which occur in steady frequencies and probably have important roles in spermatogenesis and fertilization.

Cited by 106 publications

References 21 publications

Spermatozoal transcriptome profiling for bull sperm motility: a potential tool to evaluate semen quality

Spermatozoal transcriptome profiling for bull sperm motility: a potential tool to evaluate semen quality

The role of Dby mRNA in early development of male mouse zygotes

The ’omics revolution and our understanding of sperm cell biology

Contact Info

Product

Resources

About