Characterization and quantification of chemical constituents in Angong Niuhuang Pill using ultra-high performance liquid chromatography tandem mass spectrometry

Ding, Meng; Gao, Wen; Li, Mengning; Chen, Lu; Yang, Hua; Li, Ping

doi:10.1016/j.jpba.2023.115309

Cited by 8 publications

(3 citation statements)

References 25 publications

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“…[3][4][5] Simultaneously, Sbyor-bzo-ghi-wang is also an important component of various traditional Tibetan medicine prescriptions (TTMPs). [6][7][8] Those TTMPs are used to treat central nervous system, cardiovascular, respiratory, and other related diseases. Sbyor-bzo-ghi-wang is rare and expensive, leading to counterfeit in the market.…”

Section: Introductionmentioning

confidence: 99%

“…Current studies indicate that Sbyor‐bzo‐ghi‐wang has many pharmacological effects, as it is useful to treat stroke and prostate cancer, relieves phlegm, possesses anti‐inflammatory properties, and treats dizziness and epilepsy 3–5 . Simultaneously, Sbyor‐bzo‐ghi‐wang is also an important component of various traditional Tibetan medicine prescriptions (TTMPs) 6–8 . Those TTMPs are used to treat central nervous system, cardiovascular, respiratory, and other related diseases.…”

Section: Introductionmentioning

confidence: 99%

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Rapid analysis of the chemical constituents of traditional Tibetan medicine Sbyor‐bzo‐ghi‐wang using ballpoint electrospray ionization technique

Song,

Wang,

Drolma

et al. 2024

Rapid Comm Mass Spectrometry

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RationaleThe chemical constituents of traditional Tibetan medicines (TTM) can be identified using high‐performance liquid chromatography and high‐resolution mass spectrometry (HPLC–MS/MS) technique. However, the HPLC–MS/MS technique requires the sample to be pretreated and then separated using the specific liquid chromatography method, which is time consuming. This study developed a ballpoint electrospray ionization (BPESI) technique for analyzing the chemical constituents of Sbyor‐bzo‐ghi‐wang. This technique is a simple and inexpensive method for the rapid identification of the chemical constituents of TTMs.MethodsAfter the important parameters of the homemade BPESI device were optimized, the chemical constituents of Sbyor‐bzo‐ghi‐wang were quickly identified without sample pretreatment. The raw data were converted to mzML file using MSConvert and then identified using SIRIUS 5 software.ResultsThe results showed that 30 compounds were identified from Sbyor‐bzo‐ghi‐wang, namely eight bile acids, six flavonoids, four alkaloids, three amino acids, and nine others. Compared to the ultra‐high‐performance liquid chromatography‐Q/Orbitrap and high‐resolution mass spectrometry (UHPLC‐Q/Orbitrap HRMS) technique, the BPESI technique identified almost similar types of compounds and also a comparable number of compounds.ConclusionsCompared with the traditional HPLC–MS/MS methods, the BPESI technique does not require complex sample pretreatment and subsequent chromatographic separation steps; also it consumes a small quantity of samples. Therefore, BPESI can be used for the qualitative analysis of the chemical constituents of Sbyor‐bzo‐ghi‐wang.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Rapid analysis of the chemical constituents of traditional Tibetan medicine Sbyor‐bzo‐ghi‐wang using ballpoint electrospray ionization technique

Song,

Wang,

Drolma

et al. 2024

Rapid Comm Mass Spectrometry

View full text Add to dashboard Cite

show abstract

“…A recent study characterized and quantified the chemical constituents of AGNHW using ultrahigh-performance liquid chromatography-tandem mass spectrometry and identified a total of 205 compounds, mainly bile acids, flavonoids, alkaloids and iridoid glycosides. Among them, 68 major constituents were simultaneously detected in different batches of AGNHW samples [12]. In recent years, a number of experimental studies have reported the potential neuroprotective effects of AGNHW on ischaemic injury [13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

An-Gong-Niu-Huang-Wan (AGNHW) regulates cerebral blood flow by improving hypoperfusion, cerebrovascular reactivity and microcirculation disturbances after stroke

Zhang,

Pei,

Xue

et al. 2024

Chin Med

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Background The restoration of cerebrovascular regulation and improvement of cerebral blood flow in ischaemic regions are crucial for improving the clinical prognosis after stroke. An-Gong-Niu-Huang-Wan (AGNHW) is a famous traditional compound Chinese medicine that has been used for over 220 years to treat acute ischaemic stroke; however, its role in the regulation of cerebral blood flow is still unclear. The aim of the present study was to investigate the regulatory effect of AGNHW on cerebral blood flow and microcirculation after ischaemic stroke and to elucidate the underlying mechanisms involved. Methods Male C57BL/6 mice were subjected to distal middle cerebral artery occlusion (dMCAO) and randomly assigned to the sham, MCAO, or AGNHW groups. AGNHW was administered intragastrically 1 h after dMCAO. The rotarod test was utilized to evaluate behavioural function; TTC was used to determine the infarct volume; and ischaemic injury was assessed by detecting brain levels of SOD, MDA and NO. Then, cortical perfusion and acetazolamide-induced cerebrovascular reactivity were assessed using laser speckle contrast imaging, and the velocity and flux of red blood cells in cortical capillaries were detected using two-photon laser scanning microscopy. In addition, we employed RNA-Seq to identify variations in gene expression profiles and assessed endothelium-dependent changes in microcirculatory dysfunction by measuring vasoactive mediator levels. Results AGNHW significantly increased cerebral blood flow, reduced the infarct volume, and promoted functional recovery after cerebral ischaemia. AGNHW increased the velocity and flux of red blood cells in capillaries and improved cerebrovascular reactivity in the ischaemic cortex. Furthermore, AGNHW regulated endothelium-dependent microcirculation, as evidenced by decreases in the expression of endothelins (Edn1, Edn3 and Ednrb) and the ratios of brain and serum TXB2/6-keto-PGF1α and ET-1/CGRP. Conclusions AGNHW improved cerebral hypoperfusion, regulated cerebrovascular reactivity and attenuated microcirculatory dysfunction within the ischaemic cortex after stroke. This outstanding effect was achieved by modulating the expression of genes related to vascular endothelial cell function and regulating endothelium-dependent vasoactive mediators.

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Chemical composition and absorption characteristics of Angong Niuhuang Pill based on two-dimensional ultra-high-performance liquid chromatography-quadrupole Orbitrap high-resolution mass spectrometry

Wang,

Wu,

Tang

et al. 2023

Journal of Chromatography A

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Characterization and quantification of chemical constituents in Angong Niuhuang Pill using ultra-high performance liquid chromatography tandem mass spectrometry

Cited by 8 publications

References 25 publications

Rapid analysis of the chemical constituents of traditional Tibetan medicine Sbyor‐bzo‐ghi‐wang using ballpoint electrospray ionization technique

Rapid analysis of the chemical constituents of traditional Tibetan medicine Sbyor‐bzo‐ghi‐wang using ballpoint electrospray ionization technique

An-Gong-Niu-Huang-Wan (AGNHW) regulates cerebral blood flow by improving hypoperfusion, cerebrovascular reactivity and microcirculation disturbances after stroke

Chemical composition and absorption characteristics of Angong Niuhuang Pill based on two-dimensional ultra-high-performance liquid chromatography-quadrupole Orbitrap high-resolution mass spectrometry

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