Characterization and Purification of Acid Phosphatase from Ancient Human Bone

Demir, Yaşar; Yildirim, Safinur; Alaylı, Azize; Demir, Nazan

doi:10.1081/pb-120025373

Cited by 4 publications

(3 citation statements)

References 13 publications

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“…Alkaline phosphatase (EC 3.1.3.1) exists in animates (animals, plants, microorganisms and yeasts) (1,2). This enzyme is one of the most ubiquitous enzymes and has been found in yeasts, molds and seeds of some plants, fruits and in many animal tissues such as bovine brain, kidney, liver, spleen, erythrocytes, bones, placenta, hepatocytes and blood plasma (3,4).…”

Section: Introductionmentioning

confidence: 99%

Investigating effect of Amino Acids Leucine, Valine and Alanine on Alkaline Phosphatase Activity of purified Acetone fractions of Sweet Lemon, Garlic and Onion

Tavakoli¹,

Rastegar²,

Khaledi³

et al. 2019

IJAM

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Alkaline phosphatase enzyme (ALP, ALKP, ALPase, Alk Phos) (EC 3.1.3.1) produced by plants and microbes is presumed to convert organic phosphorus into available pi, which is absorbed by plants. Considering the importance of Alkaline phosphate enzyme and the balance between its function in the development of various diseases by increasing and decreasing the enzyme level by enzyme activating and regulating compounds, including amino acids and ions, which act as coenzyme and cofactor for the enzyme Can be a major role in Disease improvement. This study aims to comparatively investigate the inhibiting effect of the three Amino Acid as well as Alanine, Leucine and valine on Alkaline phosphatase was which extracted from Sweet Lemon (Citrus limon (L.) Osbeck), Garlic (Allium sativum L.) and Onion (Allium cepa L.). In this study, Leucine, Alanine and Valine amino acids showed strong inhibitory effects 23%, 26% and 34% respectively activity of Alkaline phosphatase obtained saline extract of plants Sweet Lemon, Garlic and Onion.

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Section: Introductionmentioning

confidence: 99%

Investigating effect of Amino Acids Leucine, Valine and Alanine on Alkaline Phosphatase Activity of purified Acetone fractions of Sweet Lemon, Garlic and Onion

Tavakoli¹,

Rastegar²,

Khaledi³

et al. 2019

IJAM

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show abstract

“…ACPs are widely distributed in plants, animals, and microorganisms. They have been localized in seeds, leaves, plumules, roots, tubers, and root nodules of plants ; in bones, liver, prostate, seminal plasma, and spleen of animals ; and in mycelia, secretions, and fruiting bodies of microorganisms . Plant and microbial ACPs play important roles in the production, transportation, and recycling of inorganic phosphorus, while animal ACPs act as key enzymes for cell detection and autolysis .…”

Section: Introductionmentioning

confidence: 99%

Purification and characterization of a novel acid phosphatase from the split gill mushroom Schizophyllum commune

Zhang

Chen

Jian

et al. 2013

J. Basic Microbiol.

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A monomeric acid phosphatase (ACP) with a molecular mass of 72.5 kDa was purified from fresh fruiting bodies of cultured Schizophyllum commune mushroom. The isolation procedure entailed ion exchange chromatography on DEAE-cellulose, CM-cellulose, and Q-sepharose, and gel filtration by fast protein liquid chromatography on Superdex 75. It demonstrated a unique N-terminal amino acid sequence of NAPWAQIDEV, which exhibited 60% amino acid identity to that of S. commune hypothetical histidine ACP based on its genome sequence, but less than 30% amino acid identity to that of other fungal ACPs previously reported. The ACP exhibited an optimum temperature at 50 °C, an optimum pH at pH 4.6, and was considerably stable at a pH range of 4.0 to 9.0, and a temperature range of 20-40 °C. The Km of the purified enzyme for ρ-nitrophenyl phosphate (ρNPP) was 0.248 mM and the Vmax was 9.093 × 10(-3) μM/min. ACP activity was strongly inhibited by Al(3+) and Fe(3+) , but enhanced by Co(2+) , Mg(2+) , and Ca(2+) at a concentration of 0.5 mM.

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“…Ein Schwerpunkt liegt auf der Identifikation von Enzymen in alten Geweben, z. B. Arbeiten über Phosphatasen von Demir et al (2003), Etspüler et al (1995), Kaup et al (1994) und Weser et al (1995).…”

Section: Endogene Biomoleküleunclassified

Steroidhormone in bodengelagertem Skelettmaterial

Holger¹

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Biosyntheseweg von Steroidhormonen im menschlichen Körper Abb. 2.1.3.B Chemische Struktur von 17 β-Estradiol Abb. 2.1.3.C Veränderungen der Serum-Konzentrationen wichtiger Sexualhormone einschließlich E2 bei Eintritt in die Menopause Abb. 2.1.3.D Anstieg des E2-Spiegels in Serum während der Schwangerschaft Abb. 3.1.2.A Skizze der archäologischen Ausgrabungsfläche "Hinter den Brüdern" in Goslar. Eingezeichnet sind die ergrabenen Bestattungen, gelb markiert die Individuen, deren Knochen auf Estradiol untersucht wurden Abb. 3.2.1.B Grundriß der Lichtensteinhöhle Abb. 3.2.1.C Gemauerte Gruft der Herzogin Anna von Brandenburg im Mittelschiff der Klosterkirche zu Bordesholm Abb. 3.2.1.D Proximales Ende eines der beiden Ossa metatarsalia von BO 9 Abb. 3.2.1.E Vergleich der beiden Ossa metatarsalia von BO 9.

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Characterization and Purification of Acid Phosphatase from Ancient Human Bone

Cited by 4 publications

References 13 publications

Investigating effect of Amino Acids Leucine, Valine and Alanine on Alkaline Phosphatase Activity of purified Acetone fractions of Sweet Lemon, Garlic and Onion

Investigating effect of Amino Acids Leucine, Valine and Alanine on Alkaline Phosphatase Activity of purified Acetone fractions of Sweet Lemon, Garlic and Onion

Purification and characterization of a novel acid phosphatase from the split gill mushroom Schizophyllum commune

Steroidhormone in bodengelagertem Skelettmaterial

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