Characterization and primary structure of a human immunodeficiency virus type 1 (HIV-1) neutralization domain as presented by a poliovirus type 1/HIV-1 chimera

The neutralizing antibody response against a conserved region of human immunodeficiency virus type 1 gp41 (amino acid residues 731-752) is uniquely directed against a conformational epitope.

“…MAb 1575 specific to the epitope IEEE, and MAbs 1577, 1583 and 1908, all specific for the epitope ERDRD of the gp41 transmembrane protein of HIV-1, were kindly donated by C. Vella (Vella et al, 1993). Viruses used are described in the appropriate sections below.…”

“…The ELISA reactivities of sera from mice immunized with CPMV-HIV\1 and wt CPMV were compared with those observed for MAbs directed against the IEEE and ERDRD portions of the 735-752 sequence of gp41 (MAb 1575 andMAb 1908, respectively ;Vella et al, 1993). In the solid phase we immobilized a full panel of FHV recombinant proteins carrying either the full epitope IEEEGGERDRDRS or just the GERDRDR sequence.…”

“…Monoclonal antibodies (MAbs) against this region may neutralize by blocking the interaction between gp41 and gp41-binding molecules on human cells (Chen & Dierich, 1996). The other (Kennedy et al, 1986) is peculiarly situated in a supposedly intravirion or intracellular location, at residues ($"PRGPDRPEGIEEEGGE-RDRDRS(&# (Modrow et al, 1987 ;Gallagher, 1992), and although weakly neutralizing MAbs have been raised to it (Dalgleish et al, 1988 ;Vella et al, 1993), its function as a neutralization site is still the subject of controversy.…”

HIV-1 Glycoprotein Immunogenicity

“…The 746 ERDRD 750 epitope is exposed constitutively and does not require contact with cell receptors or an elevated temperature (Cleveland et al, 2003). 734 (Vella et al, 1993), and the neutralizing EPES (epitope purified and ERDRD specific) and SAR1 antibodies. EPES and SAR1 Abs were both obtained by immunisation of mice with cowpea mosaic virus (CPMV) chimeras expressing short, ERDRD-containing gp41 sequences.…”

Human immunodeficiency virus type 2 (HIV-2) env gene analysis: prediction of glycoprotein epitopes important for heterotypic neutralization and evidence for three genotype clusters within the HIV-2a subtype

“…An HIV-2-derived peptide encompassing residues 747-755 of our peptide has been shown to induce neutralizing antibodies in guinea-pigs (Bjorling et al, 1991) and a poliovirus/HIV chimaera expressing the HIV-1 Kennedy domain has been used to generate HIV-1 neutralizing monoclonal antibodies (Vella et al, 1993). Characterization of antibodies from the latter study showed the Kennedy domain of HIV-1LA I to contain two epitopes based on the minimal sequences IEEE and ERDRD, residues 753 756 and 759-763 respectively (Fig.…”