Characterization and Potentials of Indigenous Oil-Degrading Bacteria Inhabiting the Rhizosphere of Wild Oat (Avena Fatua L.) in South West of Iran.

Rajaei, Saeideh; Seyedi, Seyed Mahdi; Raiesi, Fayez; Shiran, Behrouz; Raheb, Jamshid

doi:10.5812/ijb.9334

Cited by 43 publications

(33 citation statements)

References 43 publications

(60 reference statements)

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“…The high resistance to aromatics might be due to the fact that xylene and toluene are the constituents of petroleum like paraffin, however, the isolate could not degrade xylene and toluene because it is possible that CMG 457 might be lacking some unknown factors essentially required for the expression of degradative genes. Our finding is contrary to Rajei et al [16] who found that Enterobacter is capable of degrading aromatic hydrocarbons as they possess aromatic catabolic pathway.…”

Section: Discussioncontrasting

confidence: 57%

Indigenous Oil Degrading Bacteria: Isolation, Screening and Characterization

Shahab

Shafi

Ahmed

2017

Natl. j. health sci.

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Background: Oil contamination is increasing at an alarming rate and to overcome to this problem the most efficient method used is biodegradation. Biodegradation is a natural mechanism that can be used effectively for degradation of recalcitrant hydrocarbon pollutants prevailed in the environment by employing environmentally friendly microbes.Methods: Strain CMG 457 was isolated from petrol pump site from Firdous Colony, Karachi and was identified as Enterobacter sp. after morphological and biochemical characterization. Strain resistance towards different organic hydrocarbons (paraffin, aromatics and pesticides) and utilization of various organic compounds as sole carbon source were analyzed on Nutrient agar. MIC of antibiotics and heavy metals was checked on Bushnell Haas (BH) agar at 37°C for 24 hrs. Results:Research investigated the role of Enterobacter sp. in biodegradation of petroleum hydrocarbons. The results showed that the bacterium is a gram-negative aerobic bacillus. Enterobacter sp. designated as CMG 457 was screened for oil degradation using BH agar. Resistance of strain against different organic hydrocarbons was tested and CMG 457 showed high resistance of upto 50% against paraffin. The Minimum Inhibitory Concentration (MIC) of heavy metals and antibiotic sensitivity were investigated for bacterial strains. Screening for antibiotic resistance revealed that CMG 457 was resistant to ampicillin and erythromycin and was able to tolerate heavy metals like CuSO 4 , CdCl 2 and CrCl 2 upto 3mM. Conclusions:From the study, it is concluded that oil contaminated areas are the best source for isolation of hydrocarbon degrading bacteria. Enterobacter sp. has a potential for oil degradation as it showed better hydrocarbon degrading ability.

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Section: Discussioncontrasting

confidence: 57%

Indigenous Oil Degrading Bacteria: Isolation, Screening and Characterization

Shahab

Shafi

Ahmed

2017

Natl. j. health sci.

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“…In additon, Stenotrophomonas was found in soil and several species have been associated in biodegradation of pesticides [29]. Previous study by Rajaei and co-workers confirmed the ability of this bacteria to degrade crude petroleum by incorporating into the rhizosphere part of wild oat, which a potential for phytoremediation [30]. Stenotrophomonas also had been isolated from petroleum and refinery site and was confirmed of its ability to degrade Methyl tert-butyl ether (MTBE)-contaminated water [31].…”

Section: Discussionmentioning

confidence: 89%

Isolation and Characterization of Biosurfactant-Producing Bacteria Isolated From Petroleum Contaminated Sites With the Potential to Be Used in Bioremediation

Zainal¹,

Omar²,

Ashaari³

2017

Sci. herit. j.

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Surfactants have been widely used to facilitate biodegradation of petroleum pollutant; particularly those are being produced by microorganisms which are also known as biosurfactant. Six bacterial isolates from petroleum contaminated sites were found to be potential hydrocarbon degrader candidates based on their ability to grow on minimal media supplemented with petroleum crude oil as sole carbon source. In addition, they also found to be having potential in producing extracellular biosurfactant based on the results of screening for biosurfactant activity. These isolates were further identified and characterized. Morphological and biochemical characteristics of these isolates have been examined and molecular identification was done by amplifying 16S rRNA gene. Three isolates were identified as the member of the genus Pseudomonas; another two isolates were the member of genus Comamonas and one isolate from the genus Stenotrophomonas. Several qualitative screening methods (microplate assay, oil displacement test, emulsification assay and drop-collapse test) showed variation of results; suggesting the need to support these findings with quantitative screening based on measurement of surface activity. BSP 6 which was found to be closely related to Comamonas aquatica was the most promising biosurfactant producer found in this study based on two positive results out of four qualitative screening methods performed.

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“…The aromatic hydrocarbons are more resistant against biodegradation than aliphatic compounds and they often cause serious problems during bioremediation; hence, it is very important to minimize contamination of the environment with the petroleum constituents (Rajaei et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…Several genes associated with bacteria have been evaluated for bioremediation processes. The catabolic gene xylE which codes for catechol 2,3-dioxygenase that cleaves the aromatic rings in PAHs is one of the genes identified in hydrocarbon degrading bacteria that are evaluated for use in hydrocarbon contaminated sites for bioremediation processes (Malkawi et al, 2009;Rajaei et al, 2013).…”

Section: Introductionmentioning

confidence: 99%