Characterization and phylogenetic analysis of Krüppel-like transcription factor (KLF) gene family in tree shrews (Tupaia belangeri chinensis)

Abstract

Krüppel-like factors (KLFs) are a family of zinc finger transcription factors regulating embryonic development and diseases. The phylogenetics of KLFs has not been studied in tree shrews, an animal lineage with a closer relationship to primates than rodents. Here, we identified 17 KLFs from Chinese tree shrew (Tupaia belangeri chinensis). KLF proteins are highly conserved among humans, monkeys, rats, mice and tree shrews compared to zebrafish and chickens. The CtBP binding site, Sin3A binding site and nuclear localization signals are largely conserved between tree shrews and human beings. Tupaia belangeri (Tb) KLF5 contains several conserved post-transcriptional modification motifs. Moreover, the mRNA and protein expression patterns of multiple tbKLFs are tissue-specific. TbKLF5, like hKLF5, significantly promotes NIH3T3 cell proliferation in vitro. These results provide insight for future studies regarding the structure and function of the tbKLF gene family.

WITHDRAWN: Genome-Wide DNA Methylome and Whole-Transcriptome Landscapes of Spontaneous Intraductal Papilloma in Tree Shrews (Tupaia Belangeri)

“…Tupaia belangeri (Tb) KLF5, similar to human Krüppel-like factor (hKLF) hKLF5, signi cantly promotes cell proliferation, playing a proproliferative and oncogenic role in breast cancer [23]. These ndings suggested that tree shrews may serve as alternative animal models for breast cancer related to KLF5 [24]. In this study, KLF family members were not included among the DEGs, but DNA methylation analysis showed that KLF5 presented signi cant upregulation among the identi ed DMRs.…”

树鼩——作为一种新颖的非人灵长类实验疾病动物模型

“…Overexpression of the PyMT oncogene by lentivirus can also efficiently induce mammary tumors in tree shrews within a month ( Ge et al, 2016 ). Furthermore, KLF5, an important transcription factor in breast cancer initiation and development, is reported to be highly conserved between tree shrews and humans ( Shao et al, 2017 ).…”

Genome-wide DNA methylome and whole-transcriptome landscapes of spontaneous intraductal papilloma in tree shrews

“…In their study, Shao et al (38) identified 17 Krüppel-like factors from Chinese tree shrews. KLF5 encodes a member of the zinc finger protein KLF subfamily which acts as transcriptional activator by binding directly to a specific recognition motif in the promoters of target genes, through which it plays roles in both promoting and suppressing cell proliferation.…”