Characterization and osteogenic potential of equine muscle tissue– and periosteal tissue–derived mesenchymal stem cells in comparison with bone marrow– and adipose tissue–derived mesenchymal stem cells

Radtke, Catherine L.; Nino‐Fong, Rodolfo; Gonzalez, Blanca P. Esparza; Stryhn, Henrik; McDuffee, Laurie A.

doi:10.2460/ajvr.74.5.790

Cited by 57 publications

(60 citation statements)

References 53 publications

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“…However, the large number of nucleated but non-adherent hematopoietic cells present in bone marrow aspirates may contribute to this finding. Cell surface markers appear to be similar in cells derived from each of the aforementioned tissues with only positivity for VEGFR-2 (Flk-1) being higher in PDC than in ADC and MDC [93]. However, this analysis was carried out in passaged cells therefore it is conceivable that these cells have lost their tissuespecific profile during culture adaptation.…”

Section: Periosteum-derived Cells Versus Mesenchymal Progenitor Cellsmentioning

confidence: 88%

Uncovering the periosteum for skeletal regeneration: The stem cell that lies beneath

Roberts

Gastel

Carmeliet

et al. 2015

Bone

208

186

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The cartilage- and bone-forming properties of the periosteum have long since been recognized. As one of the major sources of skeletal progenitor cells, the periosteum plays a crucial role not only in bone development and growth, but also during bone fracture healing. Aided by the continuous expansion of tools and techniques, we are now starting to acquire more insight into the specific role and regulation of periosteal cells. From a therapeutic point of view, the periosteum has attracted much attention as a cell source for bone tissue engineering purposes. This interest derives not only from the physiological role of the periosteum during bone repair, but is also supported by the unique properties and marked bone-forming potential of expanded periosteum-derived cells. We provide an overview of the current knowledge of periosteal cell biology, focusing on the cellular composition and molecular regulation of this remarkable tissue, as well as the application of periosteum-derived cells in regenerative medicine approaches. This article is part of a Special Issue entitled "Stem Cells and Bone".

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Section: Periosteum-derived Cells Versus Mesenchymal Progenitor Cellsmentioning

confidence: 88%

Uncovering the periosteum for skeletal regeneration: The stem cell that lies beneath

Roberts

Gastel

Carmeliet

et al. 2015

Bone

208

186

View full text Add to dashboard Cite

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“…10 BM-SCs have been useful to patients with hematological malignancies and other diseases for the past few decades. MSCs or BM-SCs offer the advantage of minimizing immune reactions to negligible levels because, as shown previously, these can be derived from the patients themselves.…”

Section: Discussionmentioning

confidence: 99%

“…9 Recent studies have elucidated that cells with similar functional and morphological features to mesenchymal BM-SCs can be isolated from various other organs and tissues such as the peripheral blood and adipose tissue. 10 Even though MSCs arise from different origins, these cells possess cellular physiological properties that are typically associated with SCs, such as self-renewability and high potentiality to differentiate into specialized cell lineages including neuronal cell lineages. 11 BM-SC therapy represents a valid alternative to repair and replace damaged or lost neurons.…”

Section: Introductionmentioning

confidence: 99%

Ex vivo differentiation of human bone marrow-derived stem cells into neuronal cell-like lineages

Al-Zoubi¹,

Altwal²,

Khalifeh³

et al. 2016

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Background:Methods to obtain safe and practical populations of stem cells (SCs) at a clinical grade that are able to differentiate into neuronal cell lineages are yet to be developed. In a previous study, we showed that mouse bone marrow-derived SCs (BM-SCs) differentiated into neuronal cell-like lineages when put in a neuronal-like environment, which is a special media supplemented with the necessary growth factors needed for the differentiation of SCs into neuronal cell-like lineages. Aim: In this study, we aim to assess the potentials of adult human CD34+ and CD133+ SCs to differentiate into neuronal cell-like lineages ex vivo when placed in a neuronal-like microenvironment. Methods: The neuronal-like microenvironment was created by culturing cells in nonhematopoietic expansion media (NHEM) supplemented with growth factors that favor differentiation into neuronal cell lineages (low-affinity nerve growth factor [LNGF], mouse spinal cord extract [mSpE], or both). Cultured cells were assessed for neuronal differentiation by cell morphologies and by expression of GFAP. Results: Our results show that culturing unpurified human BM-derived mononuclear cells (hBM-MNCs) in NHEM+LNGF+mSpE did not lead to neuronal differentiation. In contrast, culturing of purified CD34+ hBM-SCs in NHEM+LNGF+mSpE favored their differentiation into astrocyte-like cells, whereas culturing of purified CD133+ hBM-SCs in the same media favored their differentiation into neuronal-like cells. Interestingly, coculturing of CD34+ and CD133+ hBM-SCs in the same media enhanced the differentiation into astrocyte-like cells and neuronal-like cells, in addition to oligodendrocyte-like cells. Conclusion: These results suggest that a mixture of purified CD34+ and CD133+ cells may enhance the differentiation into neuronal cell-like lineages and give broader neuronal cell lineages than when each of these cell types is cultured alone. This method opens the window for the utilization of specific populations of hBM-SCs to be delivered in a purified form for the potential treatment of neurodegenerative diseases in the future.

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“…These cells had been primarily isolated in the early 1970s when Friedenstein et al discovered that a specific number of fibroblastic cells isolated from bone marrow have the capacity to form colonies in vitro and under appropriate stimulating environmental conditions, small aggregates of bone, and cartilage [15,16]. Over the years, it has become clear that MSC are not an exclusive feature of the bone marrow [17][18][19], but can also be isolated from other organs and tissues such as fat [20][21][22], skeletal muscles [23,24], and synovium [25].…”

Section: Phenotype and Differentiation Potential Of Mscmentioning

confidence: 99%

Stem Cell-Based Therapies for Osteoarthritis: From Pre-Clinical to Clinical Applications

Toumi¹,

Lespessailles²,

Mazor³

2017

Mesenchymal Stem Cells - Isolation, Characterization and Applications

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Although many surgical and pharmaceutical interventions are currently available for treating osteoarthritis (OA), restoration of normal cartilage function remains inefficient.In fact, because of the absence of vasculature within the articular cartilage (AC), the selfpotential for regeneration is very poor. Recently, researchers and clinicians have been focusing on alternative methods for cartilage preservation and repair. It has been shown that AC contains a population of stem cells or progenitor cells, similar to those found in many other adult tissues that are thought to be involved in the maintenance of tissue homeostasis. In the present chapter, we review the current status of stem cells potential in the treatment of early OA and discuss the possible origin of these cells and the role they might have in cartilage repair. We also review the recent progress in the field of chondroprogenitors in cartilage.

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Characterization and osteogenic potential of equine muscle tissue– and periosteal tissue–derived mesenchymal stem cells in comparison with bone marrow– and adipose tissue–derived mesenchymal stem cells

Abstract: Equine muscle and periosteum are sources of MSCs. Equine muscle- and periosteal-derived MSCs have osteogenic potential comparable to that of equine adipose- and bone marrow-derived MSCs, which could make them useful for tissue engineering applications in equine medicine.

Cited by 57 publications

References 53 publications

Uncovering the periosteum for skeletal regeneration: The stem cell that lies beneath

Uncovering the periosteum for skeletal regeneration: The stem cell that lies beneath

Ex vivo differentiation of human bone marrow-derived stem cells into neuronal cell-like lineages

Stem Cell-Based Therapies for Osteoarthritis: From Pre-Clinical to Clinical Applications

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