Characterization and nucleotide sequence of the gene for the common α subunit of the bovine pituitary glycoprotein hormones

Abstract

The gene coding for the common alpha subunit of the bovine pituitary glycoprotein hormones was isolated from a bovine genomic library. The gene spans roughly 16.5 kbp, contains three intervening sequences, and codes for a message of approximately 730 nucleotides. The complete coding region of the gene was sequenced as well as 315 nucleotides of 5' flanking sequence and the entire intron C. Only a single base difference was found when the sequence of the gene was compared with that of the cDNA. Genomic blotting experiments suggest the presence of a single alpha subunit gene. Comparison of the bovine and human alpha subunit genes indicated that the high level of homology observed in the coding regions has been maintained throughout the 5' and 3' untranslated regions, and at least 90 nucleotides of the 5'flanking regions. Additionally, there is an 18 base pair sequence present in both the 5' flanking and 5' untranslated regions of the gene that is homologous to a region of the chick ovalbumin gene. This ovalbumin sequence has been suggested as a binding site for the progesterone receptor-complex.

Molecular cloning of the human thyrotropin‐β subunit gene

“…The cDNA and gene for the a subunit have been cloned and the nucleotide sequences of human [3,6], mouse [7], bovine [8,9] and rat [lo] preparations have been determined.…”

Cloning of the Genes for the Pituitary Glycoprotein Hormone α and Follicle-Stimulating Hormone β Subunits in the Japanese Crested Ibis, Nipponia nippon

“…All the PGH α genes previously reported in mammals and teleosts consist of four exons and three introns (Fiddes and Goodman, 1981;Goodwin et al ., 1983;Burnside et al ., 1988;Gordon et al ., 1988;Kato et al ., 1990;Golos et al ., 1991;Huang et al ., 1992;Suzuki et al ., 1995). The exonintron structure in the ibis PGH α gene was compared with that of carp (Huang et al, 1992), mouse , bovine (Goodwin et al, 1983) and human (Fiddes and Goodman, 1981) (Fig.…”

“…The exonintron structure in the ibis PGH α gene was compared with that of carp (Huang et al, 1992), mouse , bovine (Goodwin et al, 1983) and human (Fiddes and Goodman, 1981) (Fig. 4).…”

“…Comparison of the exon-intron structure of the part of the PGH α gene in the Japanese crested ibis with those of PGH α gene of carp (Huang et al ., 1992), mouse , bovine (Goodwin et al ., 1983) and human (Fiddes and Goodman, 1981). Solid and open boxes represent the coding and the untranslated regions of exons, respectively.…”

Structural and Functional Roles of FSH and LH as Glycoproteins Regulating Reproduction in Mammalian Species

“…Within a particular species, the -subunits of all the gonadotropins are identical in amino acid sequence, as a single gene encodes the -subunit [22,23]. Target cell specificity arises from the -subunit [24,25], which is encoded by a distinct gene.…”